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Literature summary extracted from

  • Lanfranchi, E.; Pavkov-Keller, T.; Koehler, E.M.; Diepold, M.; Steiner, K.; Darnhofer, B.; Hartler, J.; Van Den Bergh, T.; Joosten, H.J.; Gruber-Khadjawi, M.; Thallinger, G.G.; Birner-Gruenberger, R.; Gruber, K.; Winkler, M.; Glieder, A.
    Enzyme discovery beyond homology a unique hydroxynitrile lyase in the Bet v1 superfamily (2017), Sci. Rep., 7, 46738 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.10
-
 Davallia tyermanii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.2.10 sitting drop vapor-diffusion method Davallia tyermanii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.10 0.3
-
 (R)-mandelonitrile pH 5.0, temperature not specified in the publication, enzyme form DtHNL1 Davallia tyermanii
4.1.2.10 0.45
-
 (R)-mandelonitrile pH 5.0, temperature not specified in the publication, enzyme form DtHNL2 Davallia tyermanii
4.1.2.10 0.63
-
 (R)-mandelonitrile pH 5.0, temperature not specified in the publication, enzyme form DtHNL4 Davallia tyermanii
4.1.2.10 0.75
-
 (R)-mandelonitrile pH 5.0, temperature not specified in the publication, enzyme form DtHNL3 Davallia tyermanii
4.1.2.10 14
-
 benzaldehyde pH 4.0, 10°C, enzyme form DtHNL1 Davallia tyermanii

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.10 Davallia tyermanii A0A1C9V3R0 hydroxynitrile lyase isoform 2
-
4.1.2.10 Davallia tyermanii A0A1C9V3R4 hydroxynitrile lyase isoform 3
-
4.1.2.10 Davallia tyermanii A0A1C9V3R6 hydroxynitrile lyase isoform 4
-
4.1.2.10 Davallia tyermanii A0A1C9V3S9 hydroxynitrile lyase isoform 1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.10
-
 Davallia tyermanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.10 (R)-mandelonitrile
-
 Davallia tyermanii cyanide + benzaldehyde
-
 r
4.1.2.10 cyanide + 1-phenylethanone
-
 Davallia tyermanii 2-hydroxy-2-phenylpropanenitrile
-
 ?
4.1.2.10 cyanide + 2-chlorobenzaldehyde
-
 Davallia tyermanii (2R)-2-(2-chlorophenyl)-2-hydroxyacetonitrile
-
 ?
4.1.2.10 cyanide + 3-phenylprop-2-enal
-
 Davallia tyermanii (2R)-2-hydroxy-4-phenylbut-3-enenitrile
-
 ?
4.1.2.10 cyanide + 3-phenylpropanal
-
 Davallia tyermanii (2R)-2-hydroxy-4-phenylbutanenitrile
-
 ?
4.1.2.10 cyanide + benzaldehyde
-
 Davallia tyermanii (R)-mandelonitrile
-
 r
4.1.2.10 cyanide + furan-2-carbaldehyde
-
 Davallia tyermanii (2S)-furan-2-yl-hydroxyacetonitrile
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.10 35
-
 enzyme form DtHNL2 Davallia tyermanii
4.1.2.10 35
-
 enzyme form DtHNL4 Davallia tyermanii
4.1.2.10 40
-
 enzyme form DtHNL1 Davallia tyermanii
4.1.2.10 40
-
 enzyme form DtHNL3 Davallia tyermanii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.10 144
-
 (R)-mandelonitrile pH 5.0, temperature not specified in the publication, enzyme form DtHNL1 Davallia tyermanii
4.1.2.10 156
-
 (R)-mandelonitrile pH 5.0, temperature not specified in the publication, enzyme form DtHNL2 Davallia tyermanii
4.1.2.10 272
-
 (R)-mandelonitrile pH 5.0, temperature not specified in the publication, enzyme form DtHNL4 Davallia tyermanii
4.1.2.10 356
-
 (R)-mandelonitrile pH 5.0, temperature not specified in the publication, enzyme form DtHNL3 Davallia tyermanii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.10 5
-
-
 Davallia tyermanii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.2.10 2.5 4 active at pH 2.5, about 80% of maximal activity at pH 4.0 Davallia tyermanii
4.1.2.10 2.5 4 active at pH 2.5, about 80% of maximal activity at pH 4.0< Davallia tyermanii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.2.10 2.5
-
 8°C, 72 h, more than 50% of activity is retained Davallia tyermanii
4.1.2.10 2.5
-
 8°C, 72 h, more than 50% of activity retained Davallia tyermanii
4.1.2.10 2.5
-
 8°C, 72 h, more than 50% of activity retains Davallia tyermanii
4.1.2.10 4
-
 8°C, 72 h, more than 50% of activity is retained Davallia tyermanii
4.1.2.10 4
-
 8°C, 72 h, more than 50% of activity retained Davallia tyermanii
4.1.2.10 4
-
 8°C, 72 h, more than 50% of activity retains Davallia tyermanii
4.1.2.10 5
-
 8°C, 72 h, more than 65% of activity is retained Davallia tyermanii
4.1.2.10 5
-
 8°C, 72 h, more than 65% of activity retained Davallia tyermanii
4.1.2.10 5
-
 8°C, 72 h, more than 65% of activity retains Davallia tyermanii

General Information

EC Number General Information Comment Organism
4.1.2.10 metabolism the enzyme is involved in the cyanogenic pathway, a widespread defense mechanism against herbivores and pathogens. Plants produce cyanogenic glycosides e.g. prunasin or amygdalin, store these compounds in different tissues, and enzymatically degrade them upon tissue disruption, in order to finally release toxic hydrogen cyanide Davallia tyermanii
4.1.2.10 physiological function the enzyme is involved in the cyanogenic pathway, a widespread defense mechanism against herbivores and pathogens. Plants produce cyanogenic glycosides e.g. prunasin or amygdalin, store these compounds in different tissues, and enzymatically degrade them upon tissue disruption, in order to finally release toxic hydrogen cyanide Davallia tyermanii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.2.10 5
-
 (R)-mandelonitrile pH 5.0, temperature not specified in the publication, enzyme form DtHNL1 Davallia tyermanii