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Literature summary extracted from
- Metabolic engineering of the pentose phosphate pathway for enhanced limonene production in the cyanobacterium Synechocystis sp. PCC 6803 (2017), Sci. Rep., 7, 17503-17512 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 5.1.3.1 | synthesis | the enzyme overexpression is part of the engineering of Synechocystis 6803 for photosynthetic limonene production | Synechocystis sp. PCC 6803 |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.1.3.1 | gene rpe, recombinant overexpression in Synechocystis sp. PCC 6803 | Synechocystis sp. PCC 6803 |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.1.3.1 | additional information | increased limonene production by genetic modifications: Two genes in the pentose phosphate pathway, rpi and rpe, are overexpressed in Synechocystis sp. PCC 6803 to divert carbon flux toward limonene production. The Abies grandis geranyl diphosphate synthase GPPS 2, that specifically produce geranyl diphosphate, is expressed to ensure sufficient geranyl diphosphate for limonene production. Codon-optimized limonene synthases from Mentha spicata and Citrus limon are heterologously expressed in Synechocystis sp. PCC 6803 to produce S-limonene and R-limonene, respectively, method overview and computational modeling | Synechocystis sp. PCC 6803 |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.3.1 | D-Ribulose 5-phosphate | Synechocystis sp. PCC 6803 | - |
D-Xylulose 5-phosphate | - |
r |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.3.1 | Synechocystis sp. PCC 6803 | P74061 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.3.1 | D-Ribulose 5-phosphate | - |
Synechocystis sp. PCC 6803 | D-Xylulose 5-phosphate | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.1.3.1 | Ribulose 5-phosphate 3-epimerase | - |
Synechocystis sp. PCC 6803 |
| 5.1.3.1 | RPE | - |
Synechocystis sp. PCC 6803 |
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Release 2023.1 (January 2023)
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