BRENDA - Enzyme Database

Distant Phe345 mutation compromises the stability and activity of Mycobacterium tuberculosis isocitrate lyase by modulating its structural flexibility

Shukla, H.; Shukla, R.; Sonkar, A.; Pandey, T.; Tripathi, T.; Sci. Rep. 7, 1058 (2017)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
4.1.3.1
F345A
the mutation leads to complete loss of the enzyme activity, compromises the enzyme stability, and modulates the structural dynamics and flexibility of the protein
Mycobacterium tuberculosis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
200000
-
gel filtration
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Mycobacterium tuberculosis
-
succinate + glyoxylate
-
-
?
4.1.3.1
isocitrate
Mycobacterium tuberculosis H37Rv
-
succinate + glyoxylate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Mycobacterium tuberculosis
P9WKK7
-
-
4.1.3.1
Mycobacterium tuberculosis H37Rv
P9WKK7
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
749329
Mycobacterium tuberculosis
succinate + glyoxylate
-
-
-
?
4.1.3.1
isocitrate
-
749329
Mycobacterium tuberculosis H37Rv
succinate + glyoxylate
-
-
-
?
4.1.3.1
threo-DL-isocitrate
-
749329
Mycobacterium tuberculosis
succinate + glyoxylate
-
-
-
?
4.1.3.1
threo-DL-isocitrate
-
749329
Mycobacterium tuberculosis H37Rv
succinate + glyoxylate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.1.3.1
homotetramer
-
Mycobacterium tuberculosis
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
4.1.3.1
F345A
the mutation leads to complete loss of the enzyme activity, compromises the enzyme stability, and modulates the structural dynamics and flexibility of the protein
Mycobacterium tuberculosis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
200000
-
gel filtration
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Mycobacterium tuberculosis
-
succinate + glyoxylate
-
-
?
4.1.3.1
isocitrate
Mycobacterium tuberculosis H37Rv
-
succinate + glyoxylate
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
749329
Mycobacterium tuberculosis
succinate + glyoxylate
-
-
-
?
4.1.3.1
isocitrate
-
749329
Mycobacterium tuberculosis H37Rv
succinate + glyoxylate
-
-
-
?
4.1.3.1
threo-DL-isocitrate
-
749329
Mycobacterium tuberculosis
succinate + glyoxylate
-
-
-
?
4.1.3.1
threo-DL-isocitrate
-
749329
Mycobacterium tuberculosis H37Rv
succinate + glyoxylate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.3.1
homotetramer
-
Mycobacterium tuberculosis