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Literature summary extracted from

  • Dai, F.; Qiao, L.; Cao, C.; Liu, X.; Tong, X.; He, S.; Hu, H.; Zhang, L.; Wu, S.; Tan, D.; Xiang, Z.; Lu, C.
    Aspartate decarboxylase is required for a normal pupa pigmentation pattern in the silkworm, Bombyx mori (2015), Sci. Rep., 5, 10885 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.11 genetic mapping, sequence comparisons and phylogenetic analysis, quantitative RT-PCR enzyme expression analysis Bombyx mori

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.11 additional information construction of a bp mutant strain 16-100 (bp/bp). In the bp mutant, a SINE-like transposon with a length of 493 bp is detected about 2.2 kb upstream of the transcriptional start site of the BmADC gene. This insertion causes a sharp reduction in BmADC transcript levels in bp mutants, leading to deficiency of beta-alanine and N-beta-alanyl dopamine (NBAD), but accumulation of dopamine. The mutant is specifically melanized only at the pupal stage. Following injection of beta-alanine into bp mutants, the color pattern is reverted to that of the wild-type silkworms. Additionally, melanic pupae resulting from knockdown of BmADC in the wild-type strain are obtained by RNAi of BmADC Bombyx mori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.11 L-aspartate Bombyx mori
-
 beta-alanine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.11 Bombyx mori H9JRC8 three wild-type strains, Dazao (+ bp/+ bp), C108 (+ bp/+ bp), and N4 (+ bp/+ bp)
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.11 larva
-
 Bombyx mori
-
4.1.1.11 additional information temporal expression pattern of BmADC from the wandering to moth stages Bombyx mori
-
4.1.1.11 pupa
-
 Bombyx mori
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.11 L-aspartate
-
 Bombyx mori beta-alanine + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.11 aspartate decarboxylase
-
 Bombyx mori
4.1.1.11 BmADC
-
 Bombyx mori

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.11 pyridoxal 5'-phosphate
-
 Bombyx mori

General Information

EC Number General Information Comment Organism
4.1.1.11 malfunction a mutation in the aspartate decarboxylase gene (BmADC) causes melanized pupae with melanization specifically only at the pupal stage, the bp mutant phenotype. In the bp mutant, a SINE-like transposon insertion causes a sharp reduction in BmADC transcript levels in bp mutants, leading to deficiency of beta-alanine and N-beta-alanyl dopamine (NBAD), but accumulation of dopamine. Enzyme knockout also leads to the melanic pupae. The color pattern is reverted to that of the wild-type silkworms following injection of beta-alanine into bp mutants. Larvaeal bp phenotype, overview. Absence of beta-alanine and excessive accumulation of dopamine in the bp mutant Bombyx mori
4.1.1.11 metabolism the enzyme is involved in the melanin metabolism pathway and in the melanin metabolism from dopamine to N-beta-alanyl dopamine (NBAD) Bombyx mori
4.1.1.11 physiological function enzyme BmADC plays a crucial role in melanin metabolism and in the pigmentation pattern of the silkworm pupal stage Bombyx mori