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Literature summary extracted from
- Transcriptome-guided discovery and functional characterization of two UDP-sugar 4-epimerase families involved in the biosynthesis of anti-tumor polysaccharides in Ornithogalum caudatum (2016), RSC Adv., 6, 37370-37384 .
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.1.3.2 | gene UGE1, transcriptome-guided search by transcriptome analysis of Ornithogalum caudatum unigenes, cloning, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of soluble His-tagged N-terminally truncated enzyme in Escherichia coli strain | Albuca bracteata |
| 5.1.3.2 | gene UGE2, transcriptome-guided search by transcriptome analysis of Ornithogalum caudatum unigenes, cloning, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of soluble His-tagged N-terminally truncated enzyme in Escherichia coli strain Transetta (DE3) | Albuca bracteata |
| 5.1.3.5 | gene UXE1, transcriptome-guided search by transcriptome analysis of Ornithogalum caudatum unigenes, cloning, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of soluble His-tagged N-terminally truncated enzyme in Escherichia coli strain BL21(DE3) by coexpression with chaperones from plasmid pET28atOcUXE1 and chaperone plasmid pGro7, and pET28atOcUXE2 and another chaperone plasmid pKJE7. The recombinant expression in absence of chaperones results in misfolded enzyme in inclusion bodies, expression of full-length His-tagged enzyme is unsuccessful. The truncated recombinant enzyme is catalytically inactive | Albuca bracteata |
| 5.1.3.5 | gene UXE2, transcriptome-guided search by transcriptome analysis of Ornithogalum caudatum unigenes, cloning, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of soluble His-tagged N-terminally truncated enzyme in Escherichia coli strain BL21(DE3) by coexpression with chaperones from plasmid pET28atOcUXE1 and chaperone plasmid pGro7, and pET28atOcUXE2 and another chaperone plasmid pKJE7. The recombinant expression in absence of chaperones results in misfolded enzyme in inclusion bodies, expression of full-length His-tagged enzyme is unsuccessful. The truncated recombinant enzyme is catalytically inactive | Albuca bracteata |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.1.3.5 | additional information | generation of truncated enzyme for expression as soluble protein. The truncated recombinant enzyme is catalytically inactive | Albuca bracteata |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 5.1.3.5 | membrane | a type I membrane protein | Albuca bracteata | 16020 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.3.2 | UDP-glucose | Albuca bracteata | - |
UDP-galactose | - |
r |  |
| 5.1.3.5 | UDP-L-arabinose | Albuca bracteata | - |
UDP-D-xylose | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.3.2 | Albuca bracteata | A0A168GPL0 | UGE1; i.e. Albuca bracteata or Ornithogalum longebracteatum | - |
| 5.1.3.2 | Albuca bracteata | A0A168GPR3 | UGE2; i.e. Albuca bracteata or Ornithogalum longebracteatum | - |
| 5.1.3.5 | Albuca bracteata | A0A168GPQ3 | UXE1; i.e. Albuca bracteata or Ornithogalum longebracteatum | - |
| 5.1.3.5 | Albuca bracteata | A0A168GPR3 | UXE2; i.e. Albuca bracteata or Ornithogalum longebracteatum | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.1.3.5 | recombinant His-tagged truncated inactive enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Albuca bracteata |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.1.3.2 | bulb | - |
Albuca bracteata | - |
| 5.1.3.2 | bulb | sterile | Albuca bracteata | - |
| 5.1.3.5 | bulb | sterile | Albuca bracteata | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.3.2 | UDP-glucose | - |
Albuca bracteata | UDP-galactose | - |
r | |
| 5.1.3.5 | additional information | enzyme UXE, has strict substrate specificity and only catalyzes the interconversion of UDP-D-Xyl and UDP-L-Ara | Albuca bracteata | ? | - |
? | |
| 5.1.3.5 | UDP-L-arabinose | - |
Albuca bracteata | UDP-D-xylose | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.1.3.5 | ? | x * 45400, about, sequence calculation | Albuca bracteata |
| 5.1.3.5 | ? | x * 46200, about, sequence calculation | Albuca bracteata |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.1.3.2 | UDP-glucose 4-epimerase | - |
Albuca bracteata |
| 5.1.3.2 | UGE | - |
Albuca bracteata |
| 5.1.3.2 | UGE1 | - |
Albuca bracteata |
| 5.1.3.2 | UGE2 | - |
Albuca bracteata |
| 5.1.3.5 | UDP-xylose 4-epimerase | - |
Albuca bracteata |
| 5.1.3.5 | Uxe | - |
Albuca bracteata |
| 5.1.3.5 | UXE1 | - |
Albuca bracteata |
| 5.1.3.5 | UXE2 | - |
Albuca bracteata |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.2 | 30 | - |
assay at | Albuca bracteata |
| 5.1.3.5 | 30 | - |
assay at | Albuca bracteata |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.2 | 7 | - |
assay at | Albuca bracteata |
| 5.1.3.5 | 7 | - |
assay at | Albuca bracteata |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 5.1.3.5 | Albuca bracteata | sequence calculation | - |
6.93 |
| 5.1.3.5 | Albuca bracteata | sequence calculation | - |
9.05 |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 5.1.3.2 | evolution | bifunctional UDP-glucose 4-epimerases, UGEs, with UDP-xylose 4-epimerase (EC 5.1.3.5) activity are conserved in vascular plants | Albuca bracteata |
| 5.1.3.2 | physiological function | the enzyme is involved in the biosynthesis of anti-tumor polysaccharides in Ornithogalum caudatum. UGE also plays a key role in carbohydrate partitioning, plant growth and development. Enzyme UGE is important in the composition of the sugar nucleotide pools | Albuca bracteata |
| 5.1.3.2 | physiological function | the enzyme is involved in the biosynthesis of anti-tumor polysaccharides in Ornithogalum caudatum. UXE also plays a key role in carbohydrate partitioning, plant growth and development. Enzyme UXE is important in the composition of the sugar nucleotide pools | Albuca bracteata |
| 5.1.3.5 | physiological function | the enzyme is involved in the biosynthesis of anti-tumor polysaccharides in Ornithogalum caudatum. Enzyme UXE also plays a key role in carbohydrate partitioning, plant growth and development. UXE is important in the composition of the sugar nucleotide pools | Albuca bracteata |
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