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Literature summary extracted from
- Characterization of a versatile nitrile hydratase of the neonicotinoid thiacloprid-degrading bacterium Ensifer meliloti CGMCC 7333 (2016), RSC Adv., 6, 15501-15508 .
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.84 | expression in Escherichia coli rosetta | Sinorhizobium meliloti |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.84 | acetone | 2% (v/v), 70.3% inhibition | Sinorhizobium meliloti |  |
| 4.2.1.84 | Cu2+ | 0.1 mM, 14.8% inhibition | Sinorhizobium meliloti | |
| 4.2.1.84 | ethanol | 2% (v/v), 68.6% inhibition | Sinorhizobium meliloti | |
| 4.2.1.84 | ethyl acetate | 2% (v/v), 76% inhibition | Sinorhizobium meliloti | |
| 4.2.1.84 | Fe2+ | 0.1 mM, 8% inhibition | Sinorhizobium meliloti | |
| 4.2.1.84 | isoamyl alcohol | 2% (v/v), 29.9% inhibition | Sinorhizobium meliloti | |
| 4.2.1.84 | Isopropanol | 2% (v/v), 35.7% inhibition | Sinorhizobium meliloti | |
| 4.2.1.84 | methanol | 2% (v/v), 19.2% inhibition | Sinorhizobium meliloti | |
| 4.2.1.84 | Zn2+ | 0.1 mM, 8% inhibition | Sinorhizobium meliloti | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.84 | 0.14 | - |
acetamiprid | pH 7.5, 30°C | Sinorhizobium meliloti | |
| 4.2.1.84 | 7.34 | - |
3-Cyanopyridine | pH 7.5, 30°C | Sinorhizobium meliloti | |
| 4.2.1.84 | 12.39 | - |
thiacloprid | pH 7.5, 30°C | Sinorhizobium meliloti | |
| 4.2.1.84 | 14.81 | - |
Benzonitrile | pH 7.5, 30°C | Sinorhizobium meliloti | |
| 4.2.1.84 | 17.91 | - |
indole-3-acetonitrile | pH 7.5, 30°C | Sinorhizobium meliloti | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.84 | Co2+ | cobalt-type nitrile hydratase, and the addition of cobalt to the cell growth broth significantly stimulates nitrile hydratase activity. Supplementing cobalt to phosphate buffer containing purified enzyme has no effect on nitrile hydratase activity | Sinorhizobium meliloti | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.84 | Sinorhizobium meliloti | V5RFK1 AND V5RE14 | - |
- |
| 4.2.1.84 | Sinorhizobium meliloti CGMCC 7333 | V5RFK1 AND V5RE14 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.84 | - |
Sinorhizobium meliloti |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.84 | 3-cyanopyridine + H2O | hydration at 5528% compared to the activity with thiacloprid | Sinorhizobium meliloti | pyridine-3-carbamide | - |
? | |
| 4.2.1.84 | 3-cyanopyridine + H2O | hydration at 5528% compared to the activity with thiacloprid | Sinorhizobium meliloti CGMCC 7333 | pyridine-3-carbamide | - |
? | |
| 4.2.1.84 | acetamiprid + H2O | hydration at 22.41% compared to the activity with thiacloprid | Sinorhizobium meliloti | (1E)-N'-carbamoyl-N-[(6-chloropyridin-3-yl)methyl]-N-methylethanimidamide | - |
? | |
| 4.2.1.84 | acetonitrile + H2O | hydration at 29.02% compared to the activity with thiacloprid | Sinorhizobium meliloti | acetamide | - |
? | |
| 4.2.1.84 | acetonitrile + H2O | hydration at 29.02% compared to the activity with thiacloprid | Sinorhizobium meliloti CGMCC 7333 | acetamide | - |
? | |
| 4.2.1.84 | benzonitrile + H2O | hydration at 2532% compared to the activity with thiacloprid | Sinorhizobium meliloti | benzamide | - |
? | |
| 4.2.1.84 | benzonitrile + H2O | hydration at 2532% compared to the activity with thiacloprid | Sinorhizobium meliloti CGMCC 7333 | benzamide | - |
? | |
| 4.2.1.84 | hexanedinitrile + H2O | hydration at 0.43% compared to the activity with thiacloprid | Sinorhizobium meliloti | ? | - |
? | |
| 4.2.1.84 | indole-3-acetonitrile + H2O | hydration at 479.79% compared to the activity with thiacloprid | Sinorhizobium meliloti | (indole-3-yl)acetamide | - |
? | |
| 4.2.1.84 | indole-3-acetonitrile + H2O | hydration at 479.79% compared to the activity with thiacloprid | Sinorhizobium meliloti CGMCC 7333 | (indole-3-yl)acetamide | - |
? | |
| 4.2.1.84 | isobutyronitrile + H2O | hydration at 3.78% compared to the activity with thiacloprid | Sinorhizobium meliloti | isobutyramide | - |
? | |
| 4.2.1.84 | succinonitrile + H2O | hydration at 13.17% compared to the activity with thiacloprid | Sinorhizobium meliloti | ? | - |
? | |
| 4.2.1.84 | succinonitrile + H2O | hydration at 13.17% compared to the activity with thiacloprid | Sinorhizobium meliloti CGMCC 7333 | ? | - |
? | |
| 4.2.1.84 | thiacloprid + H2O | - |
Sinorhizobium meliloti | 1-[(2Z)-3-[(6-chloropyridin-3-yl)methyl]-1,3-thiazolidin-2-ylidene]urea | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.84 | 40 | 50 | hydration of thiacloprid | Sinorhizobium meliloti |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.84 | 20 | 60 | 20°C: about 45% of maximal activity, 60°C: about 20% of maximal activity | Sinorhizobium meliloti |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.84 | 40 | - |
1 h, stable up to | Sinorhizobium meliloti |
| 4.2.1.84 | 50 | - |
1 h, 85% loss of activity | Sinorhizobium meliloti |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.84 | 1.04 | - |
acetamiprid | pH 7.5, 30°C | Sinorhizobium meliloti | |
| 4.2.1.84 | 131.36 | - |
thiacloprid | pH 7.5, 30°C | Sinorhizobium meliloti | |
| 4.2.1.84 | 426.68 | - |
indole-3-acetonitrile | pH 7.5, 30°C | Sinorhizobium meliloti | |
| 4.2.1.84 | 1054.36 | - |
3-Cyanopyridine | pH 7.5, 30°C | Sinorhizobium meliloti | |
| 4.2.1.84 | 1489.82 | - |
Benzonitrile | pH 7.5, 30°C | Sinorhizobium meliloti | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.84 | 7 | - |
- |
Sinorhizobium meliloti |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.84 | 6 | 9 | pH 6.0: about 60% of maximal activity, pH 9.0: about 50% of maximal activity | Sinorhizobium meliloti |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.84 | 6 | 9 | 93% activity is retained after storage at pH 6.0-9.0 for 12 h | Sinorhizobium meliloti |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.84 | 7.42 | - |
acetamiprid | pH 7.5, 30°C | Sinorhizobium meliloti | |
| 4.2.1.84 | 10.6 | - |
thiacloprid | pH 7.5, 30°C | Sinorhizobium meliloti | |
| 4.2.1.84 | 23.8 | - |
indole-3-acetonitrile | pH 7.5, 30°C | Sinorhizobium meliloti | |
| 4.2.1.84 | 100.9 | - |
Benzonitrile | pH 7.5, 30°C | Sinorhizobium meliloti | |
| 4.2.1.84 | 146.43 | - |
3-Cyanopyridine | pH 7.5, 30°C | Sinorhizobium meliloti | |
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