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Literature summary extracted from

  • Remus, B.S.; Schwer, B.; Shuman, S.
    Characterization of the tRNA ligases of pathogenic fungi Aspergillus fumigatus and Coccidioides immitis (2016), RNA, 22, 1500-1509 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.8 expressed in a Saccharomyces cerevisiae trl1DELTA mutant Coccidioides immitis
6.5.1.8 expressed in a Saccharomyces cerevisiae trl1DELTA mutant Aspergillus fumigatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.8 Mg2+ required Coccidioides immitis
6.5.1.8 Mg2+ required Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.8 (ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O Coccidioides immitis
-
 (ribonucleotide)n+m + GMP + diphosphate
-
 ?
6.5.1.8 (ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O Aspergillus fumigatus
-
 (ribonucleotide)n+m + GMP + diphosphate
-
 ?
6.5.1.8 (ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O Coccidioides immitis RS
-
 (ribonucleotide)n+m + GMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.8 Aspergillus fumigatus Q4WTD4
-
-
6.5.1.8 Coccidioides immitis J3K151
-
-
6.5.1.8 Coccidioides immitis RS J3K151
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.8 His60 Ni Superflow resin column chromatography and Superose-200 gel filtration Coccidioides immitis
6.5.1.8 His60 Ni Superflow resin column chromatography and Superose-200 gel filtration Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.8 (ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + ATP + H2O about 60% activity with 0.1 mM ATP compared to GTP Coccidioides immitis (ribonucleotide)n+m + AMP + diphosphate
-
 ?
6.5.1.8 (ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + ATP + H2O about 78% activity with 0.1 mM ATP compared to GTP Aspergillus fumigatus (ribonucleotide)n+m + AMP + diphosphate
-
 ?
6.5.1.8 (ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + ATP + H2O about 60% activity with 0.1 mM ATP compared to GTP Coccidioides immitis RS (ribonucleotide)n+m + AMP + diphosphate
-
 ?
6.5.1.8 (ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + CTP + H2O about 60% activity with 0.1 mM CTP compared to GTP Coccidioides immitis (ribonucleotide)n+m + CMP + diphosphate
-
 ?
6.5.1.8 (ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + CTP + H2O about 70% activity with 0.1 mM CTP compared to GTP Aspergillus fumigatus (ribonucleotide)n+m + CMP + diphosphate
-
 ?
6.5.1.8 (ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + CTP + H2O about 60% activity with 0.1 mM CTP compared to GTP Coccidioides immitis RS (ribonucleotide)n+m + CMP + diphosphate
-
 ?
6.5.1.8 (ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O
-
 Coccidioides immitis (ribonucleotide)n+m + GMP + diphosphate
-
 ?
6.5.1.8 (ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O
-
 Aspergillus fumigatus (ribonucleotide)n+m + GMP + diphosphate
-
 ?
6.5.1.8 (ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O about 75% activity with 0.01 mM GTP Coccidioides immitis (ribonucleotide)n+m + GMP + diphosphate
-
 ?
6.5.1.8 (ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O about 80% activity with 0.01 mM GTP Aspergillus fumigatus (ribonucleotide)n+m + GMP + diphosphate
-
 ?
6.5.1.8 (ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O
-
 Coccidioides immitis RS (ribonucleotide)n+m + GMP + diphosphate
-
 ?
6.5.1.8 (ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O about 75% activity with 0.01 mM GTP Coccidioides immitis RS (ribonucleotide)n+m + GMP + diphosphate
-
 ?
6.5.1.8 (ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + UTP + H2O about 60% activity with 0.1 mM UTP compared to GTP Coccidioides immitis (ribonucleotide)n+m + UMP + diphosphate
-
 ?
6.5.1.8 (ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + UTP + H2O about 75% activity with 0.1 mM UTP compared to GTP Aspergillus fumigatus (ribonucleotide)n+m + UMP + diphosphate
-
 ?
6.5.1.8 (ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + UTP + H2O about 60% activity with 0.1 mM UTP compared to GTP Coccidioides immitis RS (ribonucleotide)n+m + UMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.5.1.8 monomer 1 * 94200, calculated from amino acid sequence Aspergillus fumigatus
6.5.1.8 monomer 1 * 942400, calculated from amino acid sequence Coccidioides immitis

Synonyms

EC Number Synonyms Comment Organism
6.5.1.8 Trl1
-
 Coccidioides immitis
6.5.1.8 Trl1
-
 Aspergillus fumigatus

Cofactor

EC Number Cofactor Comment Organism Structure
6.5.1.8 GTP preferred cofactor Coccidioides immitis
6.5.1.8 GTP preferred cofactor Aspergillus fumigatus

General Information

EC Number General Information Comment Organism
6.5.1.8 physiological function the enzyme operates during the unfolded protein response Coccidioides immitis
6.5.1.8 physiological function the enzyme operates during the unfolded protein response Aspergillus fumigatus