Literature summary extracted from
Fujihashi, M.; Nishitani, Y.; Kiriyama, T.; Aono, R.; Sato, T.; Takai, T.; Tagashira, K.; Fukuda, W.; Atomi, H.; Imanaka, T.; Miki, K.
Mutation design of a thermophilic Rubisco based on three-dimensional structure enhances its activity at ambient temperature (2016), Proteins, 84, 1339-1346 .
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.1.1.39 |
expression in Escherichia coli |
Thermococcus kodakarensis |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
4.1.1.39 |
structure of mutant T289D with substrate analogue 2-carboxyl-D-arabinitol 1,5-bisphosphate, at 2.25 A resolution |
Thermococcus kodakarensis |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
4.1.1.39 |
M115F |
about 30% decrease in activity |
Thermococcus kodakarensis |
4.1.1.39 |
additional information |
comination of mutant T289D and mutant SP8 having a 6-amino-acid substitution in alpha-helix 6 plus mutation V330T gives 151% of wild-type specific activity |
Thermococcus kodakarensis |
4.1.1.39 |
R117A |
about 30% decrease in activity |
Thermococcus kodakarensis |
4.1.1.39 |
T289D |
124% of wild-type activity |
Thermococcus kodakarensis |
4.1.1.39 |
V161T |
about 10% residual activity |
Thermococcus kodakarensis |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.1.1.39 |
Thermococcus kodakarensis |
O93627 |
- |
- |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.1.1.39 |
rbcL |
- |
Thermococcus kodakarensis |