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Literature summary extracted from

  • Axe, J.; ORourke, K.; Kerstetter, N.; Yezdimer, E.; Chan, Y.; Chasin, A.; Boehr, D.
    Severing of a hydrogen bond disrupts amino acid networks in the catalytically active state of the alpha subunit of tryptophan synthase (2015), Protein Sci., 24, 484-494 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.20 expression in Escherichia coli Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.20 A71G mutation in alpha-subunit, about 10fold decrease in kcat/Km value compared to wild-type alpha subunit Escherichia coli
4.2.1.20 T183V substitution decreases catalytic efficiency of the alpha-subunit in the absence of the beta subunit, leading to local changes in the structural dynamics of the beta2alpha2 and beta6alpha6 loops Escherichia coli
4.2.1.20 T183V/A158G mutation in alpha-subunit, about 12fold decrease in kcat/Km value compared to wild-type alpha subunit Escherichia coli
4.2.1.20 T183V/A180G mutation in alpha-subunit, about 20fold decrease in kcat/Km value compared to wild-type alpha subunit Escherichia coli
4.2.1.20 T183V/A185G mutation in alpha-subunit, about 12fold decrease in kcat/Km value compared to wild-type alpha subunit Escherichia coli
4.2.1.20 T183V/A59G mutation in alpha-subunit, about 50fold decrease in kcat/Km value compared to wild-type alpha subunit Escherichia coli
4.2.1.20 T183V/A67G mutation in alpha-subunit, about 30fold decrease in kcat/Km value compared to wild-type alpha subunit Escherichia coli
4.2.1.20 T183V/A71G mutation in alpha-subunit, about 8fold decrease in kcat/Km value compared to wild-type alpha subunit Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.20 0.78
-
 indole alpha-subunit, mutant T183V/A185G, pH 7.6, 25°C Escherichia coli
4.2.1.20 0.8
-
 indole alpha-subunit, mutant T183V/A158G, pH 7.6, 25°C Escherichia coli
4.2.1.20 0.84
-
 indole alpha-subunit, mutant T183V/A67G, pH 7.6, 25°C Escherichia coli
4.2.1.20 0.926
-
 indole wild-type, pH 7.6, 25°C Escherichia coli
4.2.1.20 1.1
-
 indole alpha-subunit, mutant T183V, pH 7.6, 25°C Escherichia coli
4.2.1.20 1.5 2 indole alpha-subunit, mutant T183V/A180G, pH 7.6, 25°C Escherichia coli
4.2.1.20 1.7
-
 indole alpha-subunit, mutant T183V/A71G, pH 7.6, 25°C Escherichia coli
4.2.1.20 1.84
-
 indole alpha-subunit, mutant A71G, pH 7.6, 25°C Escherichia coli
4.2.1.20 3.35
-
 indole alpha-subunit, mutant T183V/A59G, pH 7.6, 25°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.20 Escherichia coli P0A877 alpha-chain
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.20 D-glyceraldehyde 3-phosphate + indole
-
 Escherichia coli 1-(indol-3-yl)glycerol 3-phosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.20 0.003
-
 indole alpha-subunit, mutant T183V/A71G, pH 7.6, 25°C Escherichia coli
4.2.1.20 0.007
-
 indole alpha-subunit, mutant T183V, pH 7.6, 25°C Escherichia coli
4.2.1.20 0.007
-
 indole alpha-subunit, mutant T183V/A158G, pH 7.6, 25°C Escherichia coli
4.2.1.20 0.007
-
 indole alpha-subunit, mutant T183V/A180G, pH 7.6, 25°C Escherichia coli
4.2.1.20 0.007
-
 indole alpha-subunit, mutant T183V/A185G, pH 7.6, 25°C Escherichia coli
4.2.1.20 0.01
-
 indole alpha-subunit, mutant T183V/A59G, pH 7.6, 25°C Escherichia coli
4.2.1.20 0.011
-
 indole alpha-subunit, mutant T183V/A67G, pH 7.6, 25°C Escherichia coli
4.2.1.20 0.02
-
 indole alpha-subunit, mutant A71G, pH 7.6, 25°C Escherichia coli
4.2.1.20 0.095
-
 indole alpha-subunit, wild-type, pH 7.6, 25°C Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.20 0.00176
-
 indole alpha-subunit, mutant T183V/A71G, pH 7.6, 25°C Escherichia coli
4.2.1.20 0.00299
-
 indole alpha-subunit, mutant T183V/A59G, pH 7.6, 25°C Escherichia coli
4.2.1.20 0.00461
-
 indole alpha-subunit, mutant T183V/A180G, pH 7.6, 25°C Escherichia coli
4.2.1.20 0.00636
-
 indole alpha-subunit, mutant T183V, pH 7.6, 25°C Escherichia coli
4.2.1.20 0.00871
-
 indole alpha-subunit, mutant T183V/A158G, pH 7.6, 25°C Escherichia coli
4.2.1.20 0.00895
-
 indole alpha-subunit, mutant T183V/A185G, pH 7.6, 25°C Escherichia coli
4.2.1.20 0.0109
-
 indole alpha-subunit, mutant A71G, pH 7.6, 25°C Escherichia coli
4.2.1.20 0.0131
-
 indole alpha-subunit, mutant T183V/A67G, pH 7.6, 25°C Escherichia coli
4.2.1.20 0.103
-
 indole wild-type, pH 7.6, 25°C Escherichia coli