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Literature summary extracted from

  • Ul Haq, I.; Muneer, B.; Hussain, Z.; Khan, M.; Afzal, S.; Majeed, S.; Akram, F.; Akmal, S.
    Thermodynamic and saccharification analysis of cloned GH12 endo-1,4-beta-glucanase from Thermotoga petrophila in a mesophilic host (2015), Protein Pept. Lett., 22, 785-794 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.4 biofuel production its thermostability, resistance to heavy metal ions and specific activity make this enzyme an interesting candidate for industrial applications Thermotoga petrophila

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 overexpressed in Escherichia coli strain BL21 Thermotoga petrophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Co2+ 1 mM, represses the enzyme activity up to 37% Thermotoga petrophila
3.2.1.4 Hg2+ 1 mM, represses the enzyme activity up to 22% Thermotoga petrophila
3.2.1.4 Mn2+ 1 mM, represses the enzyme activity up to 40% Thermotoga petrophila
3.2.1.4 additional information the enzyme displays optimal activity even in the existence of divalent and trivalent metallic cations: Ca2+, Mg2+, Pb2+, Cd2+, Cu2+, Ni2+ and Fe3+ Thermotoga petrophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 12.5
-
 carboxymethyl cellulose pH 6.0, 95°C Thermotoga petrophila

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 38000
-
 SDS-PAGE Thermotoga petrophila

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Thermotoga petrophila
-
-
-
3.2.1.4 Thermotoga petrophila DSM 13995
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Thermotoga petrophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 barley beta-glucan + H2O
-
 Thermotoga petrophila ?
-
 ?
3.2.1.4 barley beta-glucan + H2O
-
 Thermotoga petrophila DSM 13995 ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Thermotoga petrophila ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Thermotoga petrophila DSM 13995 ?
-
 ?
3.2.1.4 laminarin + H2O
-
 Thermotoga petrophila ?
-
 ?
3.2.1.4 laminarin + H2O
-
 Thermotoga petrophila DSM 13995 ?
-
 ?
3.2.1.4 additional information no activity against starch and Avicel. The recombinant enzyme saccharifies pre-treated wheat straw and bagasse to 3.32% and 3.2%, respectively after 6 h incubation at 85°C Thermotoga petrophila ?
-
 ?
3.2.1.4 additional information no activity against starch and Avicel. The recombinant enzyme saccharifies pre-treated wheat straw and bagasse to 3.32% and 3.2%, respectively after 6 h incubation at 85°C Thermotoga petrophila DSM 13995 ?
-
 ?
3.2.1.4 Whatman filter paper + H2O
-
 Thermotoga petrophila ?
-
 ?
3.2.1.4 Whatman filter paper + H2O
-
 Thermotoga petrophila DSM 13995 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 GH12 endo-1,4-beta-glucanase
-
 Thermotoga petrophila

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 95
-
-
 Thermotoga petrophila

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.4 80 100 50% of activity is found in a temperature range from 80°C to 100°C Thermotoga petrophila

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 80
-
 stable up to 8 h Thermotoga petrophila
3.2.1.4 97
-
 half-life: 6.6 min Thermotoga petrophila
3.2.1.4 98
-
 half-life: 2.73 min Thermotoga petrophila
3.2.1.4 99
-
 half-life: 1.85 min Thermotoga petrophila

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.4 209
-
 carboxymethyl cellulose pH 6.0, 80°C Thermotoga petrophila
3.2.1.4 298
-
 carboxymethyl cellulose pH 6.0, 85°C Thermotoga petrophila
3.2.1.4 356
-
 carboxymethyl cellulose pH 6.0, 90°C Thermotoga petrophila
3.2.1.4 471
-
 carboxymethyl cellulose pH 6.0, 95°C Thermotoga petrophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 6
-
 at 70°C Thermotoga petrophila

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 3 9 50% of the retention of optimum rank of activity between pH 3.0 to pH 9.0 Thermotoga petrophila

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.4 3
-
 10 min, 20% of residual enzymatic activity Thermotoga petrophila
3.2.1.4 4
-
 10 min, 50% of residual enzymatic activity is found at pH 4.0 to 7.0 Thermotoga petrophila
3.2.1.4 6
-
 10 min,the recombinant enzyme is stable at pH 6.0 (citrate phosphate buffer) Thermotoga petrophila
3.2.1.4 7
-
 10 min, 50% of residual enzymatic activity is found at pH 4.0 to 7.0 Thermotoga petrophila
3.2.1.4 10
-
 10 min, 20% of residual enzymatic activity Thermotoga petrophila
3.2.1.4 11
-
 10 min, 20% of residual enzymatic activity Thermotoga petrophila