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Literature summary extracted from

  • Banerjee, S.; Sankaran, K.
    First ever isolation of bacterial prolipoprotein diacylglyceryl transferase in single step from Lactococcus lactis (2013), Protein Expr. Purif., 87, 120-128 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.145 expressed in Escherichia coli BL21(DE3) cells Lactococcus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.145 0.02
-
 L-1-phosphatidyl-sn-glycerol at pH 8.0 and 37°C Lactococcus lactis
2.5.1.145 0.02
-
 MKATKSAVGSTLAGCSSHHHHHH at pH 8.0 and 37°C Lactococcus lactis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.145 membrane
-
 Lactococcus lactis 16020
-

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.145 Lactococcus lactis
-
-
-
2.5.1.145 Lactococcus lactis ATCC 11454
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.145 CM Sepharose column chromatography Lactococcus lactis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.145 0.01486
-
 unpurified enzyme, at pH 8.0 and 37°C Lactococcus lactis
2.5.1.145 0.2972
-
 20fold purified enzyme, at pH 8.0 and 37°C Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.145 L-1-phosphatidyl-sn-glycerol + MKATKSAVGSTLAGCSSHHHHHH
-
 Lactococcus lactis sn-glycerol 1-phosphate + MKATKSAVGSTLAG-S-1,2-diacyl-sn-glyceryl-L-cysteine-SSHHHHHH
-
 ?
2.5.1.145 L-1-phosphatidyl-sn-glycerol + MKATKSAVGSTLAGCSSHHHHHH
-
 Lactococcus lactis ATCC 11454 sn-glycerol 1-phosphate + MKATKSAVGSTLAG-S-1,2-diacyl-sn-glyceryl-L-cysteine-SSHHHHHH
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.145 ? x * 31000, SDS-PAGE Lactococcus lactis

Synonyms

EC Number Synonyms Comment Organism
2.5.1.145 LGT
-
 Lactococcus lactis
2.5.1.145 phosphatidylglycerol: prolipoproteindiacylglyceryl transferase
-
 Lactococcus lactis
2.5.1.145 prolipoprotein diacylglyceryl transferase
-
 Lactococcus lactis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.145 37
-
-
 Lactococcus lactis