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Literature summary extracted from

  • Chittur, S.V.; Chen, Y.; Davisson, V.J.
    Expression and purification of imidazole glycerol phosphate synthase from Saccharomyces cerevisiae (2000), Protein Expr. Purif., 18, 366-377 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.2.10 catalytically active yeast HIS7 is expressed in Escherichia coli under the control of T7 polymerase promoter Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.2.10 0.004
-
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide pH 7.0, 30°C Saccharomyces cerevisiae
4.3.2.10 1.7
-
L-glutamine pH 7.0, 30°C Saccharomyces cerevisiae
4.3.2.10 246
-
NH4+ pH 7.0, 30°C Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.2.10 55000
-
gel filtration Saccharomyces cerevisiae
4.3.2.10 57000
-
equilibrium sedimentation Saccharomyces cerevisiae
4.3.2.10 61000
-
SDS-PAGE Saccharomyces cerevisiae
4.3.2.10 61003
-
MALDI MS Saccharomyces cerevisiae
4.3.2.10 61082
-
calculated from sequence Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.2.10 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine Saccharomyces cerevisiae the enzyme represents a junction between histidine biosynthesis and de novo purine biosynthesis 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
?
4.3.2.10 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine Saccharomyces cerevisiae ATCC 204508 the enzyme represents a junction between histidine biosynthesis and de novo purine biosynthesis 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.3.2.10 Saccharomyces cerevisiae P33734
-
-
4.3.2.10 Saccharomyces cerevisiae ATCC 204508 P33734
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.2.10 recombinant enzyme Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
4.3.2.10 -80°C, stored without loss of activity Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.2.10 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
-
Saccharomyces cerevisiae 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
?
4.3.2.10 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine the enzyme represents a junction between histidine biosynthesis and de novo purine biosynthesis Saccharomyces cerevisiae 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
?
4.3.2.10 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
-
Saccharomyces cerevisiae ATCC 204508 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
?
4.3.2.10 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine the enzyme represents a junction between histidine biosynthesis and de novo purine biosynthesis Saccharomyces cerevisiae ATCC 204508 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
?
4.3.2.10 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + NH4+ the yeast enzyme is distinguished from the Escherichia coli IGP synthase in its utilization of ammonia as a substrate Saccharomyces cerevisiae 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H2O
-
?
4.3.2.10 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + NH4+ the yeast enzyme is distinguished from the Escherichia coli IGP synthase in its utilization of ammonia as a substrate Saccharomyces cerevisiae ATCC 204508 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H2O
-
?

Subunits

EC Number Subunits Comment Organism
4.3.2.10 monomer 1 * 61000, SDS-PAGE Saccharomyces cerevisiae
4.3.2.10 monomer 1 * 61082, calculated from sequence Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
4.3.2.10 HIS7
-
Saccharomyces cerevisiae
4.3.2.10 IGP synthase
-
Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.2.10 0.6
-
NH4+ pH 7.0, 30°C Saccharomyces cerevisiae
4.3.2.10 3.9
-
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide pH 7.0, 30°C Saccharomyces cerevisiae
4.3.2.10 5.2
-
L-glutamine pH 7.0, 30°C Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.2.10 7
-
glutamine-dependent activity Saccharomyces cerevisiae
4.3.2.10 7 8.5 NH4+-dependent activity Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
4.3.2.10 metabolism the enzyme represents a junction between histidine biosynthesis and de novo purine biosynthesis Saccharomyces cerevisiae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.3.2.10 0.024
-
NH4+ pH 7.0, 30°C Saccharomyces cerevisiae
4.3.2.10 3.1
-
L-glutamine pH 7.0, 30°C Saccharomyces cerevisiae
4.3.2.10 950
-
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide pH 7.0, 30°C Saccharomyces cerevisiae