Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Pei, X.; Chang, Y.; Shen, J.
    Cloning, expression and characterization of an endo-acting bifunctional alginate lyase of marine bacterium Wenyingzhuangia fucanilytica (2019), Protein Expr. Purif., 154, 44-51 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.3 expression in Escherichia coli Wenyingzhuangia fucanilytica
4.2.2.11 expression in Escherichia coli Wenyingzhuangia fucanilytica

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.3 Al3+ 1 mM, 83% of initial activity Wenyingzhuangia fucanilytica
4.2.2.3 Hg2+ 1 mM, 34% of initial activity Wenyingzhuangia fucanilytica
4.2.2.3 Li+ 1 mM, 65% of initial activity Wenyingzhuangia fucanilytica
4.2.2.3 Mg2+ 1 mM, 65% of initial activity Wenyingzhuangia fucanilytica
4.2.2.3 Mn2+ 1 mM, 69% of initial activity Wenyingzhuangia fucanilytica
4.2.2.3 Ni2+ 1 mM, 32% of initial activity Wenyingzhuangia fucanilytica
4.2.2.3 SDS 1 mM, 29% of initial activity Wenyingzhuangia fucanilytica
4.2.2.3 Zn2+ 1 mM, 25% of initial activity Wenyingzhuangia fucanilytica
4.2.2.11 Al3+ 1 mM, 83% of initial activity Wenyingzhuangia fucanilytica
4.2.2.11 Hg2+ 1 mM, 34% of initial activity Wenyingzhuangia fucanilytica
4.2.2.11 Li+ 1 mM, 65% of initial activity Wenyingzhuangia fucanilytica
4.2.2.11 Mg2+ 1 mM, 65% of initial activity Wenyingzhuangia fucanilytica
4.2.2.11 Mn2+ 1 mM, 69% of initial activity Wenyingzhuangia fucanilytica
4.2.2.11 Ni2+ 1 mM, 32% of initial activity Wenyingzhuangia fucanilytica
4.2.2.11 SDS 1 mM, 29% of initial activity Wenyingzhuangia fucanilytica
4.2.2.11 Zn2+ 1 mM, 25% of initial activity Wenyingzhuangia fucanilytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.3 0.0237
-
 alginate pH 8.5, 40°C Wenyingzhuangia fucanilytica
4.2.2.11 0.0237
-
 alginate pH 8.5, 40°C Wenyingzhuangia fucanilytica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.3 Ba2+ 1 mM, 113% of initial activity Wenyingzhuangia fucanilytica
4.2.2.11 Ba2+ 1 mM, 113% of initial activity Wenyingzhuangia fucanilytica

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Wenyingzhuangia fucanilytica A0A1B1Y7V0 bifunctional alginate lyase, catalyzes both reactions of EC 4.2.2.3 and EC 4.2.2.11
-
4.2.2.11 Wenyingzhuangia fucanilytica A0A1B1Y7V0 bifunctional alginate lyase, catalyzes both reactions of EC 4.2.2.3 and EC 4.2.2.11
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.3
-
 Wenyingzhuangia fucanilytica
4.2.2.11
-
 Wenyingzhuangia fucanilytica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.3 21.48
-
 substrate poly(beta-(1,4)-D-mannuronate), pH 8.0, 35°C Wenyingzhuangia fucanilytica
4.2.2.3 23.34
-
 substrate alginate, pH 8.0, 35°C Wenyingzhuangia fucanilytica
4.2.2.3 27.03
-
 substrate poly(beta-(1,4)-D-mannuronate), pH 8.0, 35°C Wenyingzhuangia fucanilytica
4.2.2.11 21.48
-
 substrate poly(beta-(1,4)-D-mannuronate), pH 8.0, 35°C Wenyingzhuangia fucanilytica
4.2.2.11 23.34
-
 substrate alginate, pH 8.0, 35°C Wenyingzhuangia fucanilytica
4.2.2.11 27.03
-
 substrate poly(beta-(1,4)-D-mannuronate), pH 8.0, 35°C Wenyingzhuangia fucanilytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 alginate
-
 Wenyingzhuangia fucanilytica unsaturated algino-oligosaccharides
-
 ?
4.2.2.3 poly(alpha-(1,4)-L-guluronate) reaction of EC 4.2.2.11 Wenyingzhuangia fucanilytica 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
 ?
4.2.2.3 poly(beta-(1,4)-D-mannuronate)
-
 Wenyingzhuangia fucanilytica 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate)
-
 ?
4.2.2.11 alginate
-
 Wenyingzhuangia fucanilytica unsaturated algino-oligosaccharides
-
 ?
4.2.2.11 poly(alpha-(1,4)-L-guluronate)
-
 Wenyingzhuangia fucanilytica 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
 ?
4.2.2.11 poly(beta-(1,4)-D-mannuronate) reaction of EC 4.2.2.3 Wenyingzhuangia fucanilytica 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate)
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.3 ? x * 33900, calculated from sequence, x * 34000, SDS-PAGe recombinant protein with His-tag Wenyingzhuangia fucanilytica
4.2.2.11 ? x * 33900, calculated from sequence, x * 34000, SDS-PAGe recombinant protein with His-tag Wenyingzhuangia fucanilytica

Synonyms

EC Number Synonyms Comment Organism
4.2.2.3 Aly7B
-
 Wenyingzhuangia fucanilytica
4.2.2.3 AXE80_11190
-
 Wenyingzhuangia fucanilytica
4.2.2.11 AXE80_11190
-
 Wenyingzhuangia fucanilytica
4.2.2.11 xadAly7B
-
 Wenyingzhuangia fucanilytica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.3 40
-
-
 Wenyingzhuangia fucanilytica
4.2.2.11 40
-
-
 Wenyingzhuangia fucanilytica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.3 25
-
 24 h, 86% residual activity Wenyingzhuangia fucanilytica
4.2.2.3 35
-
 24 h, 76% residual activity Wenyingzhuangia fucanilytica
4.2.2.11 25
-
 24 h, 86% residual activity Wenyingzhuangia fucanilytica
4.2.2.11 35
-
 24 h, 76% residual activity Wenyingzhuangia fucanilytica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.2.3 27.94
-
 alginate pH 8.5, 40°C Wenyingzhuangia fucanilytica
4.2.2.11 27.94
-
 alginate pH 8.5, 40°C Wenyingzhuangia fucanilytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.3 8.5
-
-
 Wenyingzhuangia fucanilytica
4.2.2.11 8.5
-
-
 Wenyingzhuangia fucanilytica

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.2.3 11.81
-
 alginate pH 8.5, 40°C Wenyingzhuangia fucanilytica
4.2.2.11 11.81
-
 alginate pH 8.5, 40°C Wenyingzhuangia fucanilytica