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Literature summary extracted from

  • He, X.; Zhou, X.; Yang, Z.; Xu, L.; Yu, Y.; Jia, L.; Li, G.
    Cloning, expression and purification of D-tagatose 3-epimerase gene from Escherichia coli JM109 (2015), Protein Expr. Purif., 114, 77-81 .
    View publication on PubMed

Application

EC Number Application Comment Organism
5.1.3.31 synthesis D-tagatose 3-epimerase is a useful enzyme for the production of expensive, rare sugars, e.g. D-sorbose and D-psicose, from inexpensive sugars, e.g. D-tagatose and D-fructose Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.31 gene ycjR, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.3.31 D-fructose Escherichia coli
-
 D-psicose
-
 r
5.1.3.31 D-fructose Escherichia coli JM109
-
 D-psicose
-
 r
5.1.3.31 D-tagatose Escherichia coli
-
 D-sorbose
-
 r
5.1.3.31 D-tagatose Escherichia coli JM109
-
 D-sorbose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.31 Escherichia coli A0A037YIF5
-
-
5.1.3.31 Escherichia coli JM109 A0A037YIF5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.31 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.31 D-fructose
-
 Escherichia coli D-psicose
-
 r
5.1.3.31 D-fructose
-
 Escherichia coli JM109 D-psicose
-
 r
5.1.3.31 D-tagatose
-
 Escherichia coli D-sorbose
-
 r
5.1.3.31 D-tagatose
-
 Escherichia coli JM109 D-sorbose
-
 r

Subunits

EC Number Subunits Comment Organism
5.1.3.31 ? x * 29800, recombinant His-tagged enzyme, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
5.1.3.31 DTE
-
 Escherichia coli
5.1.3.31 ycjR
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
5.1.3.31 evolution analysis of molecular clusters of DTE gene sequences, phylogenetic analysis, overview Escherichia coli