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Literature summary extracted from
- Efficient biotransformation of D-fructose to D-mannose by a thermostable D-lyxose isomerase from Thermosediminibacter oceani (2016), Process Biochem., 51, 2026-2033 .
No PubMed abstract available
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 5.3.1.7 | synthesis | under optimum conditions, 101.6 g/l D-mannose is produced from 400 g/l D-fructose after reaction for 9 h, giving a conversion yield of 25.4% | Thermosediminibacter oceani |
| 5.3.1.15 | synthesis | under optimum conditions, 101.6 g/l D-mannose is produced from 400 g/l D-fructose after reaction for 9 h, giving a conversion yield of 25.4% | Thermosediminibacter oceani |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.1.7 | Ba2+ | 1 mM, 64% residual activity | Thermosediminibacter oceani |  |
| 5.3.1.7 | Cu2+ | 1 mM, 16% residual activity | Thermosediminibacter oceani | |
| 5.3.1.7 | Zn2+ | 1 mM, 13% residual activity | Thermosediminibacter oceani | |
| 5.3.1.15 | Ba2+ | 1 mM, 64% residual activity | Thermosediminibacter oceani | |
| 5.3.1.15 | Cu2+ | 1 mM, 16% residual activity | Thermosediminibacter oceani | |
| 5.3.1.15 | Zn2+ | 1 mM, 13% residual activity | Thermosediminibacter oceani | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.7 | 15.5 | - |
D-Lyxose | pH 6.5, 65°C | Thermosediminibacter oceani | |
| 5.3.1.7 | 27.3 | - |
D-fructose | pH 6.5, 65°C | Thermosediminibacter oceani | |
| 5.3.1.7 | 32.8 | - |
D-mannose | pH 6.5, 65°C | Thermosediminibacter oceani | |
| 5.3.1.15 | 15.5 | - |
D-Lyxose | pH 6.5, 65°C | Thermosediminibacter oceani | |
| 5.3.1.15 | 27.3 | - |
D-fructose | pH 6.5, 65°C | Thermosediminibacter oceani | |
| 5.3.1.15 | 32.8 | - |
D-mannose | pH 6.5, 65°C | Thermosediminibacter oceani | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.1.7 | Co2+ | 1 mM, 9.1fold activation | Thermosediminibacter oceani | |
| 5.3.1.7 | Fe2+ | 1 mM, 2.1fold activation | Thermosediminibacter oceani | |
| 5.3.1.7 | Mn2+ | 1 mM, 12.5fold activation | Thermosediminibacter oceani | |
| 5.3.1.7 | Ni2+ | 1 mM, 11.8fold activation | Thermosediminibacter oceani | |
| 5.3.1.15 | Co2+ | 1 mM, 9.1fold activation | Thermosediminibacter oceani | |
| 5.3.1.15 | Fe2+ | 1 mM, 2.1fold activation | Thermosediminibacter oceani | |
| 5.3.1.15 | Mn2+ | 1 mM, 12.5fold activation | Thermosediminibacter oceani | |
| 5.3.1.15 | Ni2+ | 1 mM, 11.8fold activation | Thermosediminibacter oceani | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.7 | 44000 | - |
gel filtration | Thermosediminibacter oceani |
| 5.3.1.15 | 44000 | - |
gel filtration | Thermosediminibacter oceani |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.1.7 | Thermosediminibacter oceani | D9RZ53 | cf. EC 5.3.1.15 | - |
| 5.3.1.7 | Thermosediminibacter oceani DSM 16646 | D9RZ53 | cf. EC 5.3.1.15 | - |
| 5.3.1.15 | Thermosediminibacter oceani | D9RZ53 | - |
- |
| 5.3.1.15 | Thermosediminibacter oceani DSM 1664 | D9RZ53 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.7 | D-fructose | - |
Thermosediminibacter oceani | D-mannose | - |
r | |
| 5.3.1.7 | D-fructose | - |
Thermosediminibacter oceani DSM 16646 | D-mannose | - |
r | |
| 5.3.1.7 | D-fructose | - |
Thermosediminibacter oceani DSM 1664 | D-mannose | - |
r | |
| 5.3.1.7 | D-Lyxose | reaction of EC 5.3.1.15 | Thermosediminibacter oceani | D-Xylulose | - |
r | |
| 5.3.1.7 | D-Lyxose | reaction of EC 5.3.1.15 | Thermosediminibacter oceani DSM 16646 | D-Xylulose | - |
r | |
| 5.3.1.7 | D-Lyxose | reaction of EC 5.3.1.15 | Thermosediminibacter oceani DSM 1664 | D-Xylulose | - |
r | |
| 5.3.1.15 | D-fructose | reaction of EC 5.3.1.7 | Thermosediminibacter oceani | D-mannose | - |
r | |
| 5.3.1.15 | D-fructose | reaction of EC 5.3.1.7 | Thermosediminibacter oceani DSM 16646 | D-mannose | - |
r | |
| 5.3.1.15 | D-fructose | reaction of EC 5.3.1.7 | Thermosediminibacter oceani DSM 1664 | D-mannose | - |
r | |
| 5.3.1.15 | D-Lyxose | best substrate | Thermosediminibacter oceani | D-Xylulose | - |
r | |
| 5.3.1.15 | D-Lyxose | best substrate | Thermosediminibacter oceani DSM 16646 | D-Xylulose | - |
r | |
| 5.3.1.15 | D-Lyxose | best substrate | Thermosediminibacter oceani DSM 1664 | D-Xylulose | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.3.1.7 | dimer | 2 * 22000, SDS-PAGE | Thermosediminibacter oceani |
| 5.3.1.15 | dimer | 2 * 22000, SDS-PAGE | Thermosediminibacter oceani |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.7 | 65 | - |
- |
Thermosediminibacter oceani |
| 5.3.1.15 | 65 | - |
- |
Thermosediminibacter oceani |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.7 | 70 | - |
half-life 5.64 h | Thermosediminibacter oceani |
| 5.3.1.7 | 80 | - |
half-life 0.77 h | Thermosediminibacter oceani |
| 5.3.1.7 | 85 | - |
half-life 0.2 h | Thermosediminibacter oceani |
| 5.3.1.15 | 70 | - |
half-life 5.64 h | Thermosediminibacter oceani |
| 5.3.1.15 | 80 | - |
half-life 0.77 h | Thermosediminibacter oceani |
| 5.3.1.15 | 85 | - |
half-life 0.2 h | Thermosediminibacter oceani |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.7 | 17.2 | - |
D-fructose | pH 6.5, 65°C | Thermosediminibacter oceani | |
| 5.3.1.7 | 51.8 | - |
D-Lyxose | pH 6.5, 65°C | Thermosediminibacter oceani | |
| 5.3.1.7 | 94.8 | - |
D-mannose | pH 6.5, 65°C | Thermosediminibacter oceani | |
| 5.3.1.15 | 17.2 | - |
D-fructose | pH 6.5, 65°C | Thermosediminibacter oceani | |
| 5.3.1.15 | 51.8 | - |
D-Lyxose | pH 6.5, 65°C | Thermosediminibacter oceani | |
| 5.3.1.15 | 94.8 | - |
D-mannose | pH 6.5, 65°C | Thermosediminibacter oceani | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.7 | 6.5 | - |
- |
Thermosediminibacter oceani |
| 5.3.1.15 | 6.5 | - |
- |
Thermosediminibacter oceani |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.7 | 5 | 8 | more than 50% of maximum activity | Thermosediminibacter oceani |
| 5.3.1.15 | 5 | 8 | more than 50% of maximum activity | Thermosediminibacter oceani |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.7 | 0.63 | - |
D-fructose | pH 6.5, 65°C | Thermosediminibacter oceani | |
| 5.3.1.7 | 2.88 | - |
D-mannose | pH 6.5, 65°C | Thermosediminibacter oceani | |
| 5.3.1.7 | 3.35 | - |
D-Lyxose | pH 6.5, 65°C | Thermosediminibacter oceani | |
| 5.3.1.15 | 0.63 | - |
D-fructose | pH 6.5, 65°C | Thermosediminibacter oceani | |
| 5.3.1.15 | 2.88 | - |
D-mannose | pH 6.5, 65°C | Thermosediminibacter oceani | |
| 5.3.1.15 | 3.35 | - |
D-Lyxose | pH 6.5, 65°C | Thermosediminibacter oceani | |
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