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Literature summary extracted from

  • Saoudi, B.; Habbeche, A.; Kerouaz, B.; Haberra, S.; Ben Romdhane, Z.; Tichati, L.; Boudelaa, M.; Belghith, H.; Gargouri, A.; Ladjama, A.
    Purification and characterization of a new thermoalkaliphilic pectate lyase from Actinomadura keratinilytica Cpt20 (2015), Process Biochem., 50, 2259-2266 .
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.2 Cd2+ 1 mM, no residual activity Actinomadura keratinilytica
4.2.2.2 Cu2+ 1 mM, 51% of initial activity Actinomadura keratinilytica
4.2.2.2 EDTA complete inhibition Actinomadura keratinilytica
4.2.2.2 Hg2+ 1 mM, no residual activity Actinomadura keratinilytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.2 0.45
-
 Polygalacturonate pH 9.0, 55°C Actinomadura keratinilytica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.2 extracellular
-
 Actinomadura keratinilytica
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.2 Ba2+ 1 mM, 216% of initial activity Actinomadura keratinilytica
4.2.2.2 Ca2+ 1 mM, 306% of initial activity. Presence of Ca2+ enhances thermoactivity and thermostability Actinomadura keratinilytica
4.2.2.2 Co2+ 1 mM, 180% of initial activity Actinomadura keratinilytica
4.2.2.2 Fe2+ 1 mM, 126% of initial activity Actinomadura keratinilytica
4.2.2.2 Mg2+ 1 mM, 226% of initial activity Actinomadura keratinilytica
4.2.2.2 Mn2+ 1 mM, 268% of initial activity Actinomadura keratinilytica
4.2.2.2 Zn2+ 1 mM, 202% of initial activity Actinomadura keratinilytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.2 34000
-
 gel filtration Actinomadura keratinilytica

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.2 Actinomadura keratinilytica
-
-
-
4.2.2.2 Actinomadura keratinilytica Cpt20
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.2.2 culture fluid
-
 Actinomadura keratinilytica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.2 21889
-
 pH 9.0, 55°C Actinomadura keratinilytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.2 additional information best substrate: low-esterified pectin Actinomadura keratinilytica ?
-
 ?
4.2.2.2 additional information best substrate: low-esterified pectin Actinomadura keratinilytica Cpt20 ?
-
 ?
4.2.2.2 pectin 32% esterification, best substrate. At 54% esterification, about 70% of the activity with pectin of 32% esterification Actinomadura keratinilytica unsaturated oligogalacturonides
-
 ?
4.2.2.2 pectin 32% esterification, best substrate. At 54% esterification, about 70% of the activity with pectin of 32% esterification Actinomadura keratinilytica Cpt20 unsaturated oligogalacturonides
-
 ?
4.2.2.2 polygalacturonate about 75% of the activity with pectin of 32% esterification Actinomadura keratinilytica unsaturated oligogalacturonides
-
 ?
4.2.2.2 polygalacturonate about 75% of the activity with pectin of 32% esterification Actinomadura keratinilytica Cpt20 unsaturated oligogalacturonides
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.2 monomer 1 * 34000, SDS-PAGE, 1 * 34125, MALDI-TOF Actinomadura keratinilytica

Synonyms

EC Number Synonyms Comment Organism
4.2.2.2 Pel-20
-
 Actinomadura keratinilytica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.2 70
-
-
 Actinomadura keratinilytica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.2 70
-
 half-life 18 h Actinomadura keratinilytica
4.2.2.2 80
-
 half-life 12 h Actinomadura keratinilytica
4.2.2.2 90
-
 half-life 7 h Actinomadura keratinilytica
4.2.2.2 100
-
 half-life 2 h Actinomadura keratinilytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.2 10.5
-
-
 Actinomadura keratinilytica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.2 7.5 12
-
 Actinomadura keratinilytica