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Literature summary extracted from

  • Fucinos, P.; Atanes, E.; Lopez-Lopez, O.; Solaroli, M.; Cerdan, M.; Gonzalez-Siso, M.; Pastrana, L.; Rua, M.
    Cloning, expression, purification and characterization of an oligomeric His-tagged thermophilic esterase from Thermus thermophilus HB27 (2014), Process Biochem., 49, 927-935 .
No PubMed abstract available

Application

EC Number Application Comment Organism
3.1.1.1 industry the enzyme is highly thermostable (half-life of 107.9 min at 85°C), suggesting that E34Tt-His6 has potential for industrial applications Thermus thermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1 expressed in Escherichia coli as a His-tagged protein Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 71800
-
 gel filtration Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Thermus thermophilus Q72J75
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-nitrophenyl decanoate + H2O 4-nitrophenyl decanoate is the most readily hydrolyzed substrate. The activity decreases sharply with increasing the chain length, being 4-nitrophenyldecanoate hydrolyzed at five times the rate of 4-nitrophenyl hexadecanoate Thermus thermophilus 4-nitrophenol + decanoate
-
 ?
3.1.1.1 4-nitrophenyl dodecanoate + H2O 4-nitrophenyl decanoate is the most readily hydrolyzed substrate. The activity decreases sharply with increasing the chain length, being 4-nitrophenyldecanoate hydrolyzed at five times the rate of 4-nitrophenyl hexadecanoate Thermus thermophilus 4-nitrophenol + dodecanoate
-
 ?
3.1.1.1 4-nitrophenyl hexadecanoate + H2O 4-nitrophenyl decanoate is the most readily hydrolyzed substrate. The activity decreases sharply with increasing the chain length, being 4-nitrophenyldecanoate hydrolyzed at five times the rate of 4-nitrophenyl hexadecanoate Thermus thermophilus 4-nitrophenol + hexadecanoate
-
 ?
3.1.1.1 4-nitrophenyl tetradecanoate + H2O 4-nitrophenyl decanoate is the most readily hydrolyzed substrate. The activity decreases sharply with increasing the chain length, being 4-nitrophenyldecanoate hydrolyzed at five times the rate of 4-nitrophenyl hexadecanoate Thermus thermophilus 4-nitrophenol + tetradecanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 dimer 2 * 37700, SDS-PAGE Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 E34Tt
-
 Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 85
-
 half-life: 107.9 min Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 6.3
-
 His6-tagged recombinant enzyme Thermus thermophilus
3.1.1.1 8.5
-
 wild-type enzyme Thermus thermophilus