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Literature summary extracted from

  • Roland, B.P.; Graham, T.R.
    Directed evolution of a sphingomyelin flippase reveals mechanism of substrate backbone discrimination by a P4-ATPase (2016), Proc. Natl. Acad. Sci. USA, 113, E4460-E4466 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
7.6.2.1 D256E the mutant shows reduced activity with phosphatidylcholine, increased activity with phosphatidylserine, and increased activity with sphingomyelin compared to the wild type enzyme Saccharomyces cerevisiae
7.6.2.1 F213S the mutant shows increased activity with phosphatidylcholine, phosphatidylserine, and sphingomyelin compared to the wild type enzyme Saccharomyces cerevisiae
7.6.2.1 I615M the mutant shows about wild type activity Saccharomyces cerevisiae
7.6.2.1 I615S the mutation significantly increases sphingomyelin and phosphatidylserine transport compared to the wild type enzyme Saccharomyces cerevisiae
7.6.2.1 I615T the mutant shows about wild type activity Saccharomyces cerevisiae
7.6.2.1 L242S the mutant shows reduced activity with phosphatidylcholine and wild type activity with sphingomyelin Saccharomyces cerevisiae
7.6.2.1 N220C the mutant shows wild type activity with phosphatidylcholine and increased activity with sphingomyelin Saccharomyces cerevisiae
7.6.2.1 N220S the mutant shows reduced activity with phosphatidylcholine and increased activity with sphingomyelin compared to the wild type enzyme Saccharomyces cerevisiae
7.6.2.1 N220S/L242S the mutant shows reduced activity with phosphatidylcholine and increased activity with sphingomyelin compared to the wild type enzyme Saccharomyces cerevisiae
7.6.2.1 N220T the mutant shows increased activity with phosphatidylcholine and increased activity with sphingomyelin compared to the wild type enzyme Saccharomyces cerevisiae
7.6.2.1 N550S the mutant shows reduced activity with phosphatidylcholine, increased activity with phosphatidylserine, and increased activity with sphingomyelin compared to the wild type enzyme Saccharomyces cerevisiae
7.6.2.1 T254A the mutant shows reduced activity with phosphatidylcholine, increased activity with phosphatidylserine, and increased activity with sphingomyelin compared to the wild type enzyme Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.6.2.1 plasma membrane
-
 Saccharomyces cerevisiae 5886
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.6.2.1 ATP + H2O + glycerophospholipid[side 1] Saccharomyces cerevisiae
-
 ADP + phosphate + glycerophospholipid[side 2]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.6.2.1 Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.6.2.1 ATP + H2O + glycerophospholipid[side 1]
-
 Saccharomyces cerevisiae ADP + phosphate + glycerophospholipid[side 2]
-
 ?
7.6.2.1 ATP + H2O + phosphatidylcholine[side 1]
-
 Saccharomyces cerevisiae ADP + phosphate + phosphatidylcholine[side 2]
-
 ?
7.6.2.1 ATP + H2O + phosphatidylethanolamine[side 1]
-
 Saccharomyces cerevisiae ADP + phosphate + phosphatidylethanolamine[side 2]
-
 ?
7.6.2.1 ATP + H2O + phosphatidylserine[side 1] very low activity Saccharomyces cerevisiae ADP + phosphate + phosphatidylserine[side 2]
-
 ?
7.6.2.1 ATP + H2O + sphingomyelin[side 1] low activity Saccharomyces cerevisiae ADP + phosphate + sphingomyelin[side 2]
-
 ?
7.6.2.1 additional information no activity with phosphatidic acid Saccharomyces cerevisiae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.6.2.1 Dnf1
-
 Saccharomyces cerevisiae
7.6.2.1 phospholipid flippase
-
 Saccharomyces cerevisiae
7.6.2.1 sphingomyelin flippase
-
 Saccharomyces cerevisiae