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Literature summary extracted from

  • Nasuno, R.; Hirano, Y.; Itoh, T.; Hakoshima, T.; Hibi, T.; Takagi, H.
    Structural and functional analysis of the yeast n-Acetyltransferase mpr1 involved in oxidative stress tolerance via proline metabolism (2013), Proc. Natl. Acad. Sci. USA, 110, 11821-11826 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.271 expressed in Escherichia coli BL21(DE3) or B834(DE3) cells Saccharomyces cerevisiae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.271 in complex with the substrate cis-4-hydroxy-L-proline, sitting drop vapor diffusion method, using 100 mM Bistris-HCl (pH 5.5), 240 mM MgCl2, and 20.5% (w/v) PEG 3350 Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.271 N135A the mutation causes a remarkable increase in the apparent Km value for acetyl-CoA Saccharomyces cerevisiae
2.3.1.271 N135D inactive Saccharomyces cerevisiae
2.3.1.271 N178A the mutation causes a large reduction in the apparent kcat value for acetyl-CoA Saccharomyces cerevisiae
2.3.1.271 N178D inactive Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.271 L-azetidine-2-carboxylic acid
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.271 0.0043
-
 acetyl-CoA wild type enzyme, at pH 7.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.0073
-
 acetyl-CoA mutant enzyme N178A, at pH 7.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.022
-
 acetyl-CoA mutant enzyme N135A, at pH 7.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 20.9
-
 L-azetidine-2-carboxylic acid wild type enzyme, at pH 7.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 135.8
-
 L-azetidine-2-carboxylic acid mutant enzyme N178A, at pH 7.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 407
-
 L-azetidine-2-carboxylic acid mutant enzyme N135A, at pH 7.5 and 30°C Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.271 Saccharomyces cerevisiae
-
-
-
2.3.1.271 Saccharomyces cerevisiae Sigma1278b
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.271 Ni-Sepharose 6 column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.271 acetyl-CoA + cis-4-hydroxy-L-proline
-
 Saccharomyces cerevisiae CoA + N-acetyl-cis-4-hydroxy-L-proline
-
 ?
2.3.1.271 acetyl-CoA + cis-4-hydroxy-L-proline
-
 Saccharomyces cerevisiae Sigma1278b CoA + N-acetyl-cis-4-hydroxy-L-proline
-
 ?
2.3.1.271 acetyl-CoA + L-azetidine-2-carboxylic acid
-
 Saccharomyces cerevisiae CoA + N-acetyl-L-azetidine-2-carboxylic acid
-
 ?
2.3.1.271 acetyl-CoA + L-azetidine-2-carboxylic acid
-
 Saccharomyces cerevisiae Sigma1278b CoA + N-acetyl-L-azetidine-2-carboxylic acid
-
 ?
2.3.1.271 acetyl-CoA + L-DELTA1-pyrroline-5-carboxylate + H2O
-
 Saccharomyces cerevisiae CoA + N-acetyl-glutamate-gamma-semialdehyde
-
 ?
2.3.1.271 acetyl-CoA + L-DELTA1-pyrroline-5-carboxylate + H2O
-
 Saccharomyces cerevisiae Sigma1278b CoA + N-acetyl-glutamate-gamma-semialdehyde
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.271 MPR1
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.271 7.2
-
 L-azetidine-2-carboxylic acid mutant enzyme N178A, at pH 7.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 38.6
-
 L-azetidine-2-carboxylic acid mutant enzyme N135A, at pH 7.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 287.2
-
 L-azetidine-2-carboxylic acid wild type enzyme, at pH 7.5 and 30°C Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.271 0.3
-
 L-azetidine-2-carboxylic acid wild type enzyme, at pH 7.5 and 30°C Saccharomyces cerevisiae