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Literature summary extracted from
- Plant oxidosqualene metabolism cycloartenol synthase-dependent sterol biosynthesis in Nicotiana benthamiana (2014), PLoS ONE, 9, e109156-64 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.8 | gene CAS1, sequence comparisons and phylogenetic tree | Nicotiana benthamiana |
| 5.4.99.8 | gene CAS1, sequence comparisons and phylogenetic tree, recombinant expression in the Saccharomyces cerevisiae mutant erg7 that lacks an endogenous lanosterol synthase. Upon galactose-induced expression of NtCAS1, yeast cells grown in the presence of exogenous ergosterol display a sterol profile that includes cycloartenol and four other sterols in addition to ergosterol. The major sterol is 24-methylene pollinastanol that accounts for 53% of the total and represents the most probable pathway end-product generated by the yeast steroidogenic machinery, apparently being versatile enough to accept cycloartenol as a substrate | Nicotiana tabacum |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.4.99.8 | additional information | upon galactose-induced recombinant expression of NtCAS1, yeast cells grown in the presence of exogenous ergosterol display a sterol profile that includes cycloartenol and four other sterols in addition to ergosterol. The major sterol is 24-methylene pollinastanol that accounts for 53% of the total and represents the most probable pathway end-product generated by the yeast steroidogenic machinery, apparently being versatile enough to accept cycloartenol as a substrate. The sterol biosynthetic features of erg7::NtCAS1 are identical to the 9beta,19-cyclopropylsterol biosynthetic segment of protists, algae, or plants. In the presence of ergosterol, erg7 or erg7::NtCAS1 cells display a closely similar growth profile along a range of dilutions, whereas in the absence of ergosterol, only erg7::NtCAS1 is able to grow significantly when compared to yeast mutant erg7 | Nicotiana tabacum |
| 5.4.99.8 | additional information | virus-induced gene silencing (VIGS) of CAS1 in Nicotianan benthamiana based on gene specific sequences from a Nicotiana tabacum CAS1. A morphogenetic inhibition is developed by PVX::CAS1 Nicotiana benthamiana leaves | Nicotiana benthamiana |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.8 | (3S)-2,3-epoxy-2,3-dihydrosqualene | Nicotiana benthamiana | - |
cycloartenol | - |
r |  |
| 5.4.99.8 | (3S)-2,3-epoxy-2,3-dihydrosqualene | Nicotiana tabacum | - |
cycloartenol | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.8 | Nicotiana benthamiana | - |
- |
- |
| 5.4.99.8 | Nicotiana tabacum | A0A0A1ER86 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.4.99.8 | leaf | - |
Nicotiana benthamiana | - |
| 5.4.99.8 | leaf | - |
Nicotiana tabacum | - |
| 5.4.99.8 | TBY-2 cell | - |
Nicotiana tabacum | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.8 | (3S)-2,3-epoxy-2,3-dihydrosqualene | - |
Nicotiana benthamiana | cycloartenol | - |
r | |
| 5.4.99.8 | (3S)-2,3-epoxy-2,3-dihydrosqualene | - |
Nicotiana tabacum | cycloartenol | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.8 | CAS1 | - |
Nicotiana benthamiana |
| 5.4.99.8 | CAS1 | - |
Nicotiana tabacum |
| 5.4.99.8 | NbCAS | - |
Nicotiana benthamiana |
| 5.4.99.8 | NtCAS | - |
Nicotiana tabacum |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 5.4.99.8 | evolution | the enzyme belongs to the fammily of 2,3-oxidosqualene cyclases, OSCs | Nicotiana benthamiana |
| 5.4.99.8 | evolution | the enzyme belongs to the fammily of 2,3-oxidosqualene cyclases, OSCs | Nicotiana tabacum |
| 5.4.99.8 | metabolism | the plant sterol pathway exhibits a major biosynthetic difference as compared with that of metazoans. The committed sterol precursor is the pentacyclic cycloartenol (9beta,19-cyclolanost-24-en-3beta-ol) and not lanosterol (lanosta-8,24-dien-3beta-ol) | Nicotiana benthamiana |
| 5.4.99.8 | metabolism | the plant sterol pathway exhibits a major biosynthetic difference as compared with that of metazoans. The committed sterol precursor is the pentacyclic cycloartenol (9beta,19-cyclolanost-24-en-3beta-ol) and not lanosterol (lanosta-8,24-dien-3beta-ol) | Nicotiana tabacum |
| 5.4.99.8 | additional information | cell sterol profile is determind by GC-MS analysis | Nicotiana benthamiana |
| 5.4.99.8 | additional information | cell sterol profile is determined by GC-MS analysis | Nicotiana tabacum |
| 5.4.99.8 | physiological function | physiological relevance of CAS1 gene in plants, strict dependence on CAS1 of tobacco sterol biosynthesis | Nicotiana benthamiana |
| 5.4.99.8 | physiological function | physiological relevance of CAS1 gene in plants, strict dependence on CAS1 of tobacco sterol biosynthesis | Nicotiana tabacum |
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