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Literature summary extracted from

  • Cheung, V.W.; Xue, B.; Hernandez-Valladares, M.; Go, M.K.; Tung, A.; Aguda, A.H.; Robinson, R.C.; Yew, W.S.
    Identification of polyketide inhibitors targeting 3-dehydroquinate dehydratase in the shikimate pathway of Enterococcus faecalis (2014), PLoS ONE, 9, e103598 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.10 expression in Escherichia coli Enterococcus faecalis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.10 to 2.2 A resolution Enterococcus faecalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.10 7-hydroxycoumarin uncompetitive inhibitor Enterococcus faecalis
4.2.1.10 marein flavonoid polyketide, mixed type inhibitor, both inhibits DHQase and retards the growth of Enterococcus faecalis Enterococcus faecalis
4.2.1.10 rhoifolin flavonoid glycoside, uncompetitive inhibitor Enterococcus faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.10 0.065
-
 3-dehydroquinate pH 7.5, 25°C Enterococcus faecalis

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.10 Enterococcus faecalis P36923
-
-
4.2.1.10 Enterococcus faecalis ATCC 700802 P36923
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.10 3-dehydroquinate
-
 Enterococcus faecalis 3-dehydroshikimate + H2O
-
 ?
4.2.1.10 3-dehydroquinate
-
 Enterococcus faecalis ATCC 700802 3-dehydroshikimate + H2O
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.10 110
-
 3-dehydroquinate pH 7.5, 25°C Enterococcus faecalis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.10 0.019
-
 rhoifolin pH 7.5, 25°C Enterococcus faecalis
4.2.1.10 0.042
-
 7-hydroxycoumarin pH 7.5, 25°C Enterococcus faecalis
4.2.1.10 0.34
-
 marein pH 7.5, 25°C Enterococcus faecalis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.10 1700
-
 3-dehydroquinate pH 7.5, 25°C Enterococcus faecalis