Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nguyen, T.; Hong, M.; Chang, P.; Lee, B.; Yoo, S.
    Biochemical properties of L-arabinose isomerase from Clostridium hylemonae to produce D-tagatose as a functional sweetener (2018), PLoS ONE, 13, e0196099 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.4 expression in Escherichia coli as inclusion bodies [Clostridium] hylemonae

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.4 EDTA 1 mM, complete loss of activity [Clostridium] hylemonae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.4 7.7
-
 D-galactose pH 7.5, 35°C [Clostridium] hylemonae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.4 Co2+ 1 mM, 110% of initial activity [Clostridium] hylemonae
5.3.1.4 Mg2+ 1 mM, 120% of initial activity [Clostridium] hylemonae
5.3.1.4 Mn2+ 1 mM, 111% of initial activity [Clostridium] hylemonae

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.4 [Clostridium] hylemonae C0C0X3
-
-
5.3.1.4 [Clostridium] hylemonae DSM 15053 C0C0X3
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.4 0.44
-
 substrate D-galactose, pH 7.5, 35°C [Clostridium] hylemonae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.4 D-galactose
-
 [Clostridium] hylemonae D-tagatose
-
 ?
5.3.1.4 D-galactose
-
 [Clostridium] hylemonae DSM 15053 D-tagatose
-
 ?
5.3.1.4 L-arabinose
-
 [Clostridium] hylemonae L-ribulose
-
 ?
5.3.1.4 L-arabinose
-
 [Clostridium] hylemonae DSM 15053 L-ribulose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.4 ? x * 56554, calculated from sequence, x * 57000, SDS-PAGE [Clostridium] hylemonae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.4 50
-
-
 [Clostridium] hylemonae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.3.1.4 65
-
 81% residual activity [Clostridium] hylemonae
5.3.1.4 90
-
 38% residual activity [Clostridium] hylemonae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.4 45
-
 150 min, 90% residual activity [Clostridium] hylemonae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.4 28.4
-
 D-galactose pH 7.5, 35°C [Clostridium] hylemonae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.4 7 7.5
-
 [Clostridium] hylemonae