4.2.1.84

industry

biotransformation of nitrile. Nitrile hydratase from Rhodococcus rhodochrous J1 is used for industrial production of acrylamide and nicotinamide. Production of enzyme by recombinant Escherichia coli is superior to that in R. rhodochrous J1. Genetically engineered Escherichia coli can be used for industrial applications instead of Rhodococcus rhodochrous J1. High-molecular weight nitrile hydratase may be more suitable for industrial application than low-molecular weight nitrile hydratase because of its higher product tolerance, which would lead to a high product concentration; biotransformation of nitrile. Nitrile hydratase from Rhodococcus rhodochrous J1 is used for industrial production of acrylamide and nicotinamide. Production of enzyme by recombinant Escherichia coli is superior to that in R. rhodochrous J1. Genetically engineered Escherichia coli can be used for industrial applications instead of Rhodococcus rhodochrous J1. High-molecular weight nitrile hydratase may be more suitable for industrial application than low-molecular weight nitrile hydratase because of its higher product tolerance, which would lead to a high product concentration

Rhodococcus rhodochrous

4.2.1.84

overexpressed in Escherichia coli by codon-optimization, engineering of Ribosome Binding Site and spacer sequences; overexpressed in Escherichia coli by codon-optimization, engineering of Ribosome Binding Site and spacer sequences

Rhodococcus rhodochrous

4.2.1.84

3-Cyanopyridine

1 M, 40% decrease of activity, low-molecular weight nitrile hydratase; high-molecular weight nitrile hydratase show almost no inhibition at 1 M

Rhodococcus rhodochrous

4.2.1.84

nicotinamide

0.5 M, sharp decrease in activity, low-molecular weight nitrile hydratase; 1.5 M, 40% decrease of activity, high-molecular weight nitrile hydratase

Rhodococcus rhodochrous

4.2.1.84

Co2+

about 1 mol of cobalt per mol of enzyme; about 1 mol of cobalt per mol of enzyme

Rhodococcus rhodochrous

4.2.1.84

94000

gel filtration

Rhodococcus rhodochrous

4.2.1.84

504000

gel filtration

Rhodococcus rhodochrous

4.2.1.84

Rhodococcus rhodochrous

P21219 AND P21220

P21219: alpha-subunit, P21220: beta-subunit

4.2.1.84

Rhodococcus rhodochrous

P29378 AND P29379

P29378: alpha-subunit, P29379: beta-subunit

4.2.1.84

Rhodococcus rhodochrous J1

P21219 AND P21220

P21219: alpha-subunit, P21220: beta-subunit

4.2.1.84

Rhodococcus rhodochrous J1

P29378 AND P29379

P29378: alpha-subunit, P29379: beta-subunit

4.2.1.84

;

Rhodococcus rhodochrous

4.2.1.84

3-cyanopyridine + H2O

749108

Rhodococcus rhodochrous

pyridine-3-carbamide

?

4.2.1.84

3-cyanopyridine + H2O

749108

Rhodococcus rhodochrous J1

pyridine-3-carbamide

?

4.2.1.84

oligomer

alpha10beta10, 10 * 22800 (alpha) + 10 * 26300 (beta), calculated from sequence

Rhodococcus rhodochrous

4.2.1.84

tetramer

alpha2beta2, 2 * 22800 (alpha) + 2 * 25200 (beta), calculated from sequence

Rhodococcus rhodochrous

4.2.1.84

25

assay at; assay at

Rhodococcus rhodochrous

4.2.1.84

50

10 min, 60% loss of activity high-molecular weight nitrile hydratase; 40 min, 40% loss of activity low-molecular weight nitrile hydratase

Rhodococcus rhodochrous

4.2.1.84

7.4

assay at; assay at

Rhodococcus rhodochrous

4.2.1.84

industry

biotransformation of nitrile. Nitrile hydratase from Rhodococcus rhodochrous J1 is used for industrial production of acrylamide and nicotinamide. Production of enzyme by recombinant Escherichia coli is superior to that in R. rhodochrous J1. Genetically engineered Escherichia coli can be used for industrial applications instead of Rhodococcus rhodochrous J1. High-molecular weight nitrile hydratase may be more suitable for industrial application than low-molecular weight nitrile hydratase because of its higher product tolerance, which would lead to a high product concentration

Rhodococcus rhodochrous

4.2.1.84

overexpressed in Escherichia coli by codon-optimization, engineering of Ribosome Binding Site and spacer sequences

Rhodococcus rhodochrous

4.2.1.84

3-Cyanopyridine

1 M, 40% decrease of activity, low-molecular weight nitrile hydratase

Rhodococcus rhodochrous

4.2.1.84

3-Cyanopyridine

high-molecular weight nitrile hydratase show almost no inhibition at 1 M

Rhodococcus rhodochrous

4.2.1.84

nicotinamide

0.5 M, sharp decrease in activity, low-molecular weight nitrile hydratase

Rhodococcus rhodochrous

4.2.1.84

nicotinamide

1.5 M, 40% decrease of activity, high-molecular weight nitrile hydratase

Rhodococcus rhodochrous

4.2.1.84

Co2+

about 1 mol of cobalt per mol of enzyme

Rhodococcus rhodochrous

4.2.1.84

94000

gel filtration

Rhodococcus rhodochrous

4.2.1.84

504000

gel filtration

Rhodococcus rhodochrous

4.2.1.84

Rhodococcus rhodochrous

4.2.1.84

3-cyanopyridine + H2O

749108

Rhodococcus rhodochrous

pyridine-3-carbamide

?

4.2.1.84

3-cyanopyridine + H2O

749108

Rhodococcus rhodochrous J1

pyridine-3-carbamide

?

4.2.1.84

oligomer

alpha10beta10, 10 * 22800 (alpha) + 10 * 26300 (beta), calculated from sequence

Rhodococcus rhodochrous

4.2.1.84

tetramer

alpha2beta2, 2 * 22800 (alpha) + 2 * 25200 (beta), calculated from sequence

Rhodococcus rhodochrous

4.2.1.84

25

assay at

Rhodococcus rhodochrous

4.2.1.84

50

40 min, 40% loss of activity low-molecular weight nitrile hydratase

Rhodococcus rhodochrous

4.2.1.84

50

10 min, 60% loss of activity high-molecular weight nitrile hydratase

Rhodococcus rhodochrous

4.2.1.84

7.4

assay at

Rhodococcus rhodochrous