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Literature summary extracted from

  • Lan, Y.; Zhang, X.; Liu, Z.; Zhou, L.; Shen, R.; Zhong, X.; Cui, W.; Zhou, Z.
    Overexpression and characterization of two types of nitrile hydratases from Rhodococcus rhodochrous J1 (2017), PLoS ONE, 12, e0179833 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.2.1.84 industry biotransformation of nitrile. Nitrile hydratase from Rhodococcus rhodochrous J1 is used for industrial production of acrylamide and nicotinamide. Production of enzyme by recombinant Escherichia coli is superior to that in R. rhodochrous J1. Genetically engineered Escherichia coli can be used for industrial applications instead of Rhodococcus rhodochrous J1. High-molecular weight nitrile hydratase may be more suitable for industrial application than low-molecular weight nitrile hydratase because of its higher product tolerance, which would lead to a high product concentration Rhodococcus rhodochrous

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.84 overexpressed in Escherichia coli by codon-optimization, engineering of Ribosome Binding Site and spacer sequences Rhodococcus rhodochrous

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.84 3-Cyanopyridine 1 M, 40% decrease of activity, low-molecular weight nitrile hydratase; high-molecular weight nitrile hydratase show almost no inhibition at 1 M Rhodococcus rhodochrous
4.2.1.84 nicotinamide 0.5 M, sharp decrease in activity, low-molecular weight nitrile hydratase; 1.5 M, 40% decrease of activity, high-molecular weight nitrile hydratase Rhodococcus rhodochrous

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.84 Co2+ about 1 mol of cobalt per mol of enzyme Rhodococcus rhodochrous

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.84 94000
-
 gel filtration Rhodococcus rhodochrous
4.2.1.84 504000
-
 gel filtration Rhodococcus rhodochrous

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.84 Rhodococcus rhodochrous P21219 AND P21220 P21219: alpha-subunit, P21220: beta-subunit
-
4.2.1.84 Rhodococcus rhodochrous P29378 AND P29379 P29378: alpha-subunit, P29379: beta-subunit
-
4.2.1.84 Rhodococcus rhodochrous J1 P21219 AND P21220 P21219: alpha-subunit, P21220: beta-subunit
-
4.2.1.84 Rhodococcus rhodochrous J1 P29378 AND P29379 P29378: alpha-subunit, P29379: beta-subunit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.84
-
 Rhodococcus rhodochrous

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.84 3-cyanopyridine + H2O
-
 Rhodococcus rhodochrous pyridine-3-carbamide
-
 ?
4.2.1.84 3-cyanopyridine + H2O
-
 Rhodococcus rhodochrous J1 pyridine-3-carbamide
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.84 oligomer alpha10beta10, 10 * 22800 (alpha) + 10 * 26300 (beta), calculated from sequence Rhodococcus rhodochrous
4.2.1.84 tetramer alpha2beta2, 2 * 22800 (alpha) + 2 * 25200 (beta), calculated from sequence Rhodococcus rhodochrous

Synonyms

EC Number Synonyms Comment Organism
4.2.1.84 H-NHase
-
 Rhodococcus rhodochrous
4.2.1.84 high-molecular weight nitrile hydratase
-
 Rhodococcus rhodochrous
4.2.1.84 L-Nhase
-
 Rhodococcus rhodochrous
4.2.1.84 low-molecular weight nitrile hydratase
-
 Rhodococcus rhodochrous

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.84 25
-
 assay at Rhodococcus rhodochrous

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.84 50
-
 10 min, 60% loss of activity high-molecular weight nitrile hydratase Rhodococcus rhodochrous
4.2.1.84 50
-
 40 min, 40% loss of activity low-molecular weight nitrile hydratase Rhodococcus rhodochrous

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.84 7.4
-
 assay at Rhodococcus rhodochrous