BRENDA - Enzyme Database show

Overexpression and characterization of two types of nitrile hydratases from Rhodococcus rhodochrous J1

Lan, Y.; Zhang, X.; Liu, Z.; Zhou, L.; Shen, R.; Zhong, X.; Cui, W.; Zhou, Z.; PLoS ONE 12, e0179833 (2017)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
4.2.1.84
industry
biotransformation of nitrile. Nitrile hydratase from Rhodococcus rhodochrous J1 is used for industrial production of acrylamide and nicotinamide. Production of enzyme by recombinant Escherichia coli is superior to that in R. rhodochrous J1. Genetically engineered Escherichia coli can be used for industrial applications instead of Rhodococcus rhodochrous J1. High-molecular weight nitrile hydratase may be more suitable for industrial application than low-molecular weight nitrile hydratase because of its higher product tolerance, which would lead to a high product concentration; biotransformation of nitrile. Nitrile hydratase from Rhodococcus rhodochrous J1 is used for industrial production of acrylamide and nicotinamide. Production of enzyme by recombinant Escherichia coli is superior to that in R. rhodochrous J1. Genetically engineered Escherichia coli can be used for industrial applications instead of Rhodococcus rhodochrous J1. High-molecular weight nitrile hydratase may be more suitable for industrial application than low-molecular weight nitrile hydratase because of its higher product tolerance, which would lead to a high product concentration
Rhodococcus rhodochrous
Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.84
overexpressed in Escherichia coli by codon-optimization, engineering of Ribosome Binding Site and spacer sequences; overexpressed in Escherichia coli by codon-optimization, engineering of Ribosome Binding Site and spacer sequences
Rhodococcus rhodochrous
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.84
3-Cyanopyridine
1 M, 40% decrease of activity, low-molecular weight nitrile hydratase; high-molecular weight nitrile hydratase show almost no inhibition at 1 M
Rhodococcus rhodochrous
4.2.1.84
nicotinamide
0.5 M, sharp decrease in activity, low-molecular weight nitrile hydratase; 1.5 M, 40% decrease of activity, high-molecular weight nitrile hydratase
Rhodococcus rhodochrous
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.84
Co2+
about 1 mol of cobalt per mol of enzyme; about 1 mol of cobalt per mol of enzyme
Rhodococcus rhodochrous
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.84
94000
-
gel filtration
Rhodococcus rhodochrous
4.2.1.84
504000
-
gel filtration
Rhodococcus rhodochrous
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.84
Rhodococcus rhodochrous
P21219 AND P21220
P21219: alpha-subunit, P21220: beta-subunit
-
4.2.1.84
Rhodococcus rhodochrous
P29378 AND P29379
P29378: alpha-subunit, P29379: beta-subunit
-
4.2.1.84
Rhodococcus rhodochrous J1
P21219 AND P21220
P21219: alpha-subunit, P21220: beta-subunit
-
4.2.1.84
Rhodococcus rhodochrous J1
P29378 AND P29379
P29378: alpha-subunit, P29379: beta-subunit
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.84
;
Rhodococcus rhodochrous
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.84
3-cyanopyridine + H2O
-
749108
Rhodococcus rhodochrous
pyridine-3-carbamide
-
-
-
?
4.2.1.84
3-cyanopyridine + H2O
-
749108
Rhodococcus rhodochrous J1
pyridine-3-carbamide
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.84
oligomer
alpha10beta10, 10 * 22800 (alpha) + 10 * 26300 (beta), calculated from sequence
Rhodococcus rhodochrous
4.2.1.84
tetramer
alpha2beta2, 2 * 22800 (alpha) + 2 * 25200 (beta), calculated from sequence
Rhodococcus rhodochrous
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.84
25
-
assay at; assay at
Rhodococcus rhodochrous
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.2.1.84
50
-
10 min, 60% loss of activity high-molecular weight nitrile hydratase; 40 min, 40% loss of activity low-molecular weight nitrile hydratase
Rhodococcus rhodochrous
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.84
7.4
-
assay at; assay at
Rhodococcus rhodochrous
Application (protein specific)
EC Number
Application
Commentary
Organism
4.2.1.84
industry
biotransformation of nitrile. Nitrile hydratase from Rhodococcus rhodochrous J1 is used for industrial production of acrylamide and nicotinamide. Production of enzyme by recombinant Escherichia coli is superior to that in R. rhodochrous J1. Genetically engineered Escherichia coli can be used for industrial applications instead of Rhodococcus rhodochrous J1. High-molecular weight nitrile hydratase may be more suitable for industrial application than low-molecular weight nitrile hydratase because of its higher product tolerance, which would lead to a high product concentration
Rhodococcus rhodochrous
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.84
overexpressed in Escherichia coli by codon-optimization, engineering of Ribosome Binding Site and spacer sequences
Rhodococcus rhodochrous
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.84
3-Cyanopyridine
1 M, 40% decrease of activity, low-molecular weight nitrile hydratase
Rhodococcus rhodochrous
4.2.1.84
3-Cyanopyridine
high-molecular weight nitrile hydratase show almost no inhibition at 1 M
Rhodococcus rhodochrous
4.2.1.84
nicotinamide
0.5 M, sharp decrease in activity, low-molecular weight nitrile hydratase
Rhodococcus rhodochrous
4.2.1.84
nicotinamide
1.5 M, 40% decrease of activity, high-molecular weight nitrile hydratase
Rhodococcus rhodochrous
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.84
Co2+
about 1 mol of cobalt per mol of enzyme
Rhodococcus rhodochrous
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.84
94000
-
gel filtration
Rhodococcus rhodochrous
4.2.1.84
504000
-
gel filtration
Rhodococcus rhodochrous
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.84
-
Rhodococcus rhodochrous
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.84
3-cyanopyridine + H2O
-
749108
Rhodococcus rhodochrous
pyridine-3-carbamide
-
-
-
?
4.2.1.84
3-cyanopyridine + H2O
-
749108
Rhodococcus rhodochrous J1
pyridine-3-carbamide
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.84
oligomer
alpha10beta10, 10 * 22800 (alpha) + 10 * 26300 (beta), calculated from sequence
Rhodococcus rhodochrous
4.2.1.84
tetramer
alpha2beta2, 2 * 22800 (alpha) + 2 * 25200 (beta), calculated from sequence
Rhodococcus rhodochrous
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.84
25
-
assay at
Rhodococcus rhodochrous
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.2.1.84
50
-
40 min, 40% loss of activity low-molecular weight nitrile hydratase
Rhodococcus rhodochrous
4.2.1.84
50
-
10 min, 60% loss of activity high-molecular weight nitrile hydratase
Rhodococcus rhodochrous
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.84
7.4
-
assay at
Rhodococcus rhodochrous