Data extracted from this reference:
Application
4.2.1.84
industry
biotransformation of nitrile. Nitrile hydratase from Rhodococcus rhodochrous J1 is used for industrial production of acrylamide and nicotinamide. Production of enzyme by recombinant Escherichia coli is superior to that in R. rhodochrous J1. Genetically engineered Escherichia coli can be used for industrial applications instead of Rhodococcus rhodochrous J1. High-molecular weight nitrile hydratase may be more suitable for industrial application than low-molecular weight nitrile hydratase because of its higher product tolerance, which would lead to a high product concentration; biotransformation of nitrile. Nitrile hydratase from Rhodococcus rhodochrous J1 is used for industrial production of acrylamide and nicotinamide. Production of enzyme by recombinant Escherichia coli is superior to that in R. rhodochrous J1. Genetically engineered Escherichia coli can be used for industrial applications instead of Rhodococcus rhodochrous J1. High-molecular weight nitrile hydratase may be more suitable for industrial application than low-molecular weight nitrile hydratase because of its higher product tolerance, which would lead to a high product concentration
Rhodococcus rhodochrous
Cloned(Commentary)
4.2.1.84
overexpressed in Escherichia coli by codon-optimization, engineering of Ribosome Binding Site and spacer sequences; overexpressed in Escherichia coli by codon-optimization, engineering of Ribosome Binding Site and spacer sequences
Rhodococcus rhodochrous
Inhibitors
4.2.1.84
3-Cyanopyridine
1 M, 40% decrease of activity, low-molecular weight nitrile hydratase; high-molecular weight nitrile hydratase show almost no inhibition at 1 M
Rhodococcus rhodochrous
4.2.1.84
nicotinamide
0.5 M, sharp decrease in activity, low-molecular weight nitrile hydratase; 1.5 M, 40% decrease of activity, high-molecular weight nitrile hydratase
Rhodococcus rhodochrous
Metals/Ions
4.2.1.84
Co2+
about 1 mol of cobalt per mol of enzyme; about 1 mol of cobalt per mol of enzyme
Rhodococcus rhodochrous
Molecular Weight [Da]
4.2.1.84
94000
gel filtration
Rhodococcus rhodochrous
4.2.1.84
504000
gel filtration
Rhodococcus rhodochrous
Organism
4.2.1.84
Rhodococcus rhodochrous
P21219 AND P21220
P21219: alpha-subunit, P21220: beta-subunit
4.2.1.84
Rhodococcus rhodochrous
P29378 AND P29379
P29378: alpha-subunit, P29379: beta-subunit
4.2.1.84
Rhodococcus rhodochrous J1
P21219 AND P21220
P21219: alpha-subunit, P21220: beta-subunit
4.2.1.84
Rhodococcus rhodochrous J1
P29378 AND P29379
P29378: alpha-subunit, P29379: beta-subunit
Purification (Commentary)
4.2.1.84
;
Rhodococcus rhodochrous
Substrates and Products (Substrate)
4.2.1.84
3-cyanopyridine + H2O
749108
Rhodococcus rhodochrous
pyridine-3-carbamide
?
4.2.1.84
3-cyanopyridine + H2O
749108
Rhodococcus rhodochrous J1
pyridine-3-carbamide
?
Subunits
4.2.1.84
oligomer
alpha10beta10, 10 * 22800 (alpha) + 10 * 26300 (beta), calculated from sequence
Rhodococcus rhodochrous
4.2.1.84
tetramer
alpha2beta2, 2 * 22800 (alpha) + 2 * 25200 (beta), calculated from sequence
Rhodococcus rhodochrous
Synonyms
4.2.1.84
H-NHase
Rhodococcus rhodochrous
4.2.1.84
high-molecular weight nitrile hydratase
Rhodococcus rhodochrous
4.2.1.84
L-Nhase
Rhodococcus rhodochrous
4.2.1.84
low-molecular weight nitrile hydratase
Rhodococcus rhodochrous
Temperature Optimum [°C]
4.2.1.84
25
assay at; assay at
Rhodococcus rhodochrous
Temperature Stability [°C]
4.2.1.84
50
10 min, 60% loss of activity high-molecular weight nitrile hydratase; 40 min, 40% loss of activity low-molecular weight nitrile hydratase
Rhodococcus rhodochrous
pH Optimum
4.2.1.84
7.4
assay at; assay at
Rhodococcus rhodochrous
Application (protein specific)
4.2.1.84
industry
biotransformation of nitrile. Nitrile hydratase from Rhodococcus rhodochrous J1 is used for industrial production of acrylamide and nicotinamide. Production of enzyme by recombinant Escherichia coli is superior to that in R. rhodochrous J1. Genetically engineered Escherichia coli can be used for industrial applications instead of Rhodococcus rhodochrous J1. High-molecular weight nitrile hydratase may be more suitable for industrial application than low-molecular weight nitrile hydratase because of its higher product tolerance, which would lead to a high product concentration
Rhodococcus rhodochrous
Cloned(Commentary) (protein specific)
4.2.1.84
overexpressed in Escherichia coli by codon-optimization, engineering of Ribosome Binding Site and spacer sequences
Rhodococcus rhodochrous
Inhibitors (protein specific)
4.2.1.84
3-Cyanopyridine
1 M, 40% decrease of activity, low-molecular weight nitrile hydratase
Rhodococcus rhodochrous
4.2.1.84
3-Cyanopyridine
high-molecular weight nitrile hydratase show almost no inhibition at 1 M
Rhodococcus rhodochrous
4.2.1.84
nicotinamide
0.5 M, sharp decrease in activity, low-molecular weight nitrile hydratase
Rhodococcus rhodochrous
4.2.1.84
nicotinamide
1.5 M, 40% decrease of activity, high-molecular weight nitrile hydratase
Rhodococcus rhodochrous
Metals/Ions (protein specific)
4.2.1.84
Co2+
about 1 mol of cobalt per mol of enzyme
Rhodococcus rhodochrous
Molecular Weight [Da] (protein specific)
4.2.1.84
94000
gel filtration
Rhodococcus rhodochrous
4.2.1.84
504000
gel filtration
Rhodococcus rhodochrous
Purification (Commentary) (protein specific)
4.2.1.84
Rhodococcus rhodochrous
Substrates and Products (Substrate) (protein specific)
4.2.1.84
3-cyanopyridine + H2O
749108
Rhodococcus rhodochrous
pyridine-3-carbamide
?
4.2.1.84
3-cyanopyridine + H2O
749108
Rhodococcus rhodochrous J1
pyridine-3-carbamide
?
Subunits (protein specific)
4.2.1.84
oligomer
alpha10beta10, 10 * 22800 (alpha) + 10 * 26300 (beta), calculated from sequence
Rhodococcus rhodochrous
4.2.1.84
tetramer
alpha2beta2, 2 * 22800 (alpha) + 2 * 25200 (beta), calculated from sequence
Rhodococcus rhodochrous
Temperature Optimum [°C] (protein specific)
4.2.1.84
25
assay at
Rhodococcus rhodochrous
Temperature Stability [°C] (protein specific)
4.2.1.84
50
40 min, 40% loss of activity low-molecular weight nitrile hydratase
Rhodococcus rhodochrous
4.2.1.84
50
10 min, 60% loss of activity high-molecular weight nitrile hydratase
Rhodococcus rhodochrous
pH Optimum (protein specific)
4.2.1.84
7.4
assay at
Rhodococcus rhodochrous