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Literature summary extracted from

  • Raouf, J.; Rafique, N.; Goodman, M.; Idborg, H.; Bergqvist, F.; Armstrong, R.; Jakobsson, P.; Morgenstern, R.; Spahiu, L.
    Arg126 and Asp49 are essential for the catalytic function of microsomal prostaglandin E2 synthase 1 and Ser127 is not (2016), PLoS ONE, 11, e163600 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.99.3 expressed in Escherichia coli BL21Star (DE) pLysS cells and SF9 insect cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
5.3.99.3 D49A inactive Homo sapiens
5.3.99.3 R126A inactive Homo sapiens
5.3.99.3 R126L inactive Homo sapiens
5.3.99.3 R73A the mutant retains 50% activity compared to the wild type enzyme Homo sapiens
5.3.99.3 R73L inactive Homo sapiens
5.3.99.3 S127A the mutant retains 63% activity compared to the wild type enzyme Homo sapiens
5.3.99.3 S127C the mutant displays 80% lowered activity Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.3.99.3 microsome
-
 Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.99.3 Prostaglandin H2 Homo sapiens
-
 Prostaglandin E2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.99.3 Homo sapiens O14684
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.99.3 Ni-NTA column chromatography and Sephacryl gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.99.3 Prostaglandin H2
-
 Homo sapiens Prostaglandin E2
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.99.3 ? x * 17000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
5.3.99.3 microsomal prostaglandin E synthase 1
-
 Homo sapiens
5.3.99.3 MPGES1
-
 Homo sapiens