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Literature summary extracted from

  • Wang, Y.; Yi, L.; Wang, S.; Fan, H.; Ding, C.; Mao, X.; Lu, C.
    Crystal structure and identification of two key amino acids involved in AI-2 production and biofilm formation in Streptococcus suis LuxS (2015), PLoS ONE, 10, e0138826 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.21 expression in Escherichia coli Streptococcus suis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.4.1.21 to 1.93 A resolution Streptococcus suis

Protein Variants

EC Number Protein Variants Comment Organism
4.4.1.21 C82A inactive, mutation decreases autoinducer AI-2 production and biofilm formation Streptococcus suis
4.4.1.21 C82S 43fold decrease in activity, mutation decreases autoinducer AI-2 production and biofilm formation Streptococcus suis
4.4.1.21 F80M 7fold decrease in activity, mutation decreases autoinducer AI-2 production and biofilm formation Streptococcus suis
4.4.1.21 F80M/H87Y 42fold decrease in activity, mutation decreases autoinducer AI-2 production and biofilm formation Streptococcus suis
4.4.1.21 H87Y 37fold decrease in activity, mutation decreases autoinducer AI-2 production and biofilm formation Streptococcus suis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.21 0.0164
-
 S-ribosylhomocysteine wild-type, pH 7.5, 37°C Streptococcus suis
4.4.1.21 0.0223
-
 S-ribosylhomocysteine mutant F80M, pH 7.5, 37°C Streptococcus suis
4.4.1.21 0.0577
-
 S-ribosylhomocysteine mutant C82S, pH 7.5, 37°C Streptococcus suis
4.4.1.21 0.0604
-
 S-ribosylhomocysteine mutant F80M/H87Y, pH 7.5, 37°C Streptococcus suis
4.4.1.21 0.0708
-
 S-ribosylhomocysteine mutant H87Y, pH 7.5, 37°C Streptococcus suis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.4.1.21 Zn2+ calculated molar ratio of Zn2+ ion to each LuxS monomer is about 1:1 Streptococcus suis

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.21 Streptococcus suis B2CMA5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.21 recombinant protein Streptococcus suis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.21 S-ribosylhomocysteine
-
 Streptococcus suis L-homocysteine + 4,5-dihydroxy-2,3-pentanedione
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4.1.21 0.009
-
 S-ribosylhomocysteine mutant F80M/H87Y, pH 7.5, 37°C Streptococcus suis
4.4.1.21 0.01
-
 S-ribosylhomocysteine mutant C82S, pH 7.5, 37°C Streptococcus suis
4.4.1.21 0.011
-
 S-ribosylhomocysteine mutant H87Y, pH 7.5, 37°C Streptococcus suis
4.4.1.21 0.02
-
 S-ribosylhomocysteine mutant F80M, pH 7.5, 37°C Streptococcus suis
4.4.1.21 0.102
-
 S-ribosylhomocysteine wild-type, pH 7.5, 37°C Streptococcus suis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.4.1.21 0.149
-
 S-ribosylhomocysteine mutant F80M/H87Y, pH 7.5, 37°C Streptococcus suis
4.4.1.21 0.153
-
 S-ribosylhomocysteine mutant H87Y, pH 7.5, 37°C Streptococcus suis
4.4.1.21 0.168
-
 S-ribosylhomocysteine mutant C82S, pH 7.5, 37°C Streptococcus suis
4.4.1.21 0.882
-
 S-ribosylhomocysteine mutant F80M, pH 7.5, 37°C Streptococcus suis
4.4.1.21 6.21
-
 S-ribosylhomocysteine wild-type, pH 7.5, 37°C Streptococcus suis