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Literature summary extracted from

  • Bozic, D.; Papaefthimiou, D.; Brueckner, K.; de Vos, R.C.; Tsoleridis, C.A.; Katsarou, D.; Papanikolaou, A.; Pateraki, I.; Chatzopoulou, F.M.; Dimitriadou, E.; Kostas, S.; Manzano, D.; Scheler, U.; Ferrer, A.; Tissier, A.; Makris, A.M.; Kampranis, S.C.; Kanellis, A.K.
    Towards elucidating carnosic acid biosynthesis in Lamiaceae Functional characterization of the three first steps of the pathway in Salvia fruticosa and Rosmarinus officinalis (2015), PLoS ONE, 10, e0124106 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.175 expression in Saccharomyces cerevisiae Salvia rosmarinus
1.14.14.175 expression in Saccharomyces cerevisiae Salvia fruticosa
4.2.3.131 gene KSL, DNA and amino acid sequence determination and analysis, phylogenetic analysis, real-time PCR enzyme expression analysis, recombinant expression of HA-His-tandem-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIL, functional transient recombinant coexpression of Salvia fruticosa (+)-copalyl diphosphate sythase (SfCPS) and Salvia fruticosa ent-kaurene synthase-like (SfKSL) in Nicotiana benthamiana via transfection by Agrobacterium tumefaciens leading to production of miltiradiene in planta, NMR product analysis Salvia fruticosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.131 Mg2+ required Salvia fruticosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.131 (+)-copalyl diphosphate Salvia fruticosa
-
 miltiradiene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.175 Salvia fruticosa A0A0C5QRZ2
-
-
1.14.14.175 Salvia rosmarinus A0A0C5Q4Y6 isoform FS1
-
1.14.14.175 Salvia rosmarinus A0A0C5QEW3 isoform FS2
-
4.2.3.131 Salvia fruticosa A0A0C5QH30 genotypes France, Vrysses, and Kavoussi
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.131 recombinant HA-His-tandem-tagged enzyme from Escherichia coli strain BL21-CodonPlus (DE3)-RIL by nickel affinity chromatography Salvia fruticosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.131 leaf young leaves show higher accumulation of SfKSL transcripts, when compared to the fully expanded leaves, consistent with the trichome and developmental regulation of carnosic acid build-up Salvia fruticosa
-
4.2.3.131 additional information real-time PCR analysis demonstrates higher expression level of SfKSL in isolated trichomes than in leaves without trichomes Salvia fruticosa
-
4.2.3.131 trichome glandular, transcriptome analysis, overview Salvia fruticosa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.175 abieta-8,11,13-triene + [reduced NADPH-hemoprotein reductase] + O2
-
 Salvia rosmarinus ferruginol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.175 abieta-8,11,13-triene + [reduced NADPH-hemoprotein reductase] + O2
-
 Salvia fruticosa ferruginol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
4.2.3.131 (+)-copalyl diphosphate
-
 Salvia fruticosa miltiradiene + diphosphate
-
 ?
4.2.3.131 additional information synthesis of miltiradiene through the enzyme is confirmed by 1D and 2D NMR analyses, GC-MS analysis, overview Salvia fruticosa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.175 FS1
-
 Salvia rosmarinus
1.14.14.175 FS2
-
 Salvia rosmarinus
4.2.3.131 ent-kaurene synthase-like
-
 Salvia fruticosa
4.2.3.131 KSL
-
 Salvia fruticosa
4.2.3.131 SfKSL
-
 Salvia fruticosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.131 37
-
 assay at Salvia fruticosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.131 7
-
 assay at Salvia fruticosa

General Information

EC Number General Information Comment Organism
1.14.14.175 physiological function transient expression in Nicothiana benthamiana leads to synthese of ferruginol in leaf Salvia rosmarinus
1.14.14.175 physiological function transient expression in Nicothiana benthamiana leads to synthese of ferruginol in leaf Salvia fruticosa
4.2.3.131 physiological function enzyme SfKSL is responsible for the formation of the carnosic acid precursor miltiradiene Salvia fruticosa