BRENDA - Enzyme Database

Cofactor-independent phosphoglycerate mutase from nematodes has limited druggability, as revealed by two high-throughput screens

Crowther, G.; Booker, M.; He, M.; Li, T.; Raverdy, S.; Novelli, J.; He, P.; Dale, N.; Fife, A.; Barker Jr., R.; Kramer, M.; Van Voorhis, W.; Carlow, C.; Wang, M.; PLoS Negl. Trop. Dis. 8, e2628 (2014)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.2.12
1,10-phenanthrolin-5-amine
-
Brugia malayi
5.4.2.12
1,10-phenanthrolin-5-amine
-
Caenorhabditis elegans
5.4.2.12
2-cyclohexyl-5-ethylthieno[3,2-d][1,2]thiazol-2-ium
-
Brugia malayi
5.4.2.12
2-cyclohexyl-5-ethylthieno[3,2-d][1,2]thiazol-2-ium
-
Caenorhabditis elegans
5.4.2.12
Genzyme-2
-
Brugia malayi
5.4.2.12
Genzyme-2
-
Caenorhabditis elegans
5.4.2.12
NCDS-1
-
Brugia malayi
5.4.2.12
NCDS-1
-
Caenorhabditis elegans
5.4.2.12
Tannic acid
-
Brugia malayi
5.4.2.12
Tannic acid
-
Caenorhabditis elegans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.2.12
0.37
-
3-phospho-D-glycerate
at pH 7.9 and 20°C
Brugia malayi
5.4.2.12
0.38
-
3-phospho-D-glycerate
at pH 7.9 and 20°C
Caenorhabditis elegans
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.12
2-phospho-D-glycerate
Caenorhabditis elegans
-
3-phospho-D-glycerate
-
-
r
5.4.2.12
2-phospho-D-glycerate
Brugia malayi
-
3-phospho-D-glycerate
-
-
r
5.4.2.12
3-phospho-D-glycerate
Caenorhabditis elegans
-
2-phospho-D-glycerate
-
-
r
5.4.2.12
3-phospho-D-glycerate
Brugia malayi
-
2-phospho-D-glycerate
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.2.12
Brugia malayi
Q4VWF8
-
-
5.4.2.12
Caenorhabditis elegans
G5EFZ1
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.12
2-phospho-D-glycerate
-
749047
Caenorhabditis elegans
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
2-phospho-D-glycerate
-
749047
Brugia malayi
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
749047
Caenorhabditis elegans
2-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
749047
Brugia malayi
2-phospho-D-glycerate
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
5.4.2.12
?
x * 57000, SDS-PAGE
Brugia malayi
5.4.2.12
?
x * 59000, SDS-PAGE
Caenorhabditis elegans
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.12
additional information
the enzyme is independent from 2,3-bisphosphoglycerate
Brugia malayi
5.4.2.12
additional information
the enzyme is independent from 2,3-bisphosphoglycerate
Caenorhabditis elegans
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
5.4.2.12
0.0067
-
pH and temperature not specified in the publication
Caenorhabditis elegans
2-cyclohexyl-5-ethylthieno[3,2-d][1,2]thiazol-2-ium
5.4.2.12
0.0104
-
pH and temperature not specified in the publication
Brugia malayi
2-cyclohexyl-5-ethylthieno[3,2-d][1,2]thiazol-2-ium
5.4.2.12
0.0383
-
pH and temperature not specified in the publication
Brugia malayi
1,10-phenanthrolin-5-amine
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.12
additional information
the enzyme is independent from 2,3-bisphosphoglycerate
Brugia malayi
5.4.2.12
additional information
the enzyme is independent from 2,3-bisphosphoglycerate
Caenorhabditis elegans
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
5.4.2.12
0.0067
-
pH and temperature not specified in the publication
Caenorhabditis elegans
2-cyclohexyl-5-ethylthieno[3,2-d][1,2]thiazol-2-ium
5.4.2.12
0.0104
-
pH and temperature not specified in the publication
Brugia malayi
2-cyclohexyl-5-ethylthieno[3,2-d][1,2]thiazol-2-ium
5.4.2.12
0.0383
-
pH and temperature not specified in the publication
Brugia malayi
1,10-phenanthrolin-5-amine
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.2.12
1,10-phenanthrolin-5-amine
-
Brugia malayi
5.4.2.12
1,10-phenanthrolin-5-amine
-
Caenorhabditis elegans
5.4.2.12
2-cyclohexyl-5-ethylthieno[3,2-d][1,2]thiazol-2-ium
-
Brugia malayi
5.4.2.12
2-cyclohexyl-5-ethylthieno[3,2-d][1,2]thiazol-2-ium
-
Caenorhabditis elegans
5.4.2.12
Genzyme-2
-
Brugia malayi
5.4.2.12
Genzyme-2
-
Caenorhabditis elegans
5.4.2.12
NCDS-1
-
Brugia malayi
5.4.2.12
NCDS-1
-
Caenorhabditis elegans
5.4.2.12
Tannic acid
-
Brugia malayi
5.4.2.12
Tannic acid
-
Caenorhabditis elegans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.2.12
0.37
-
3-phospho-D-glycerate
at pH 7.9 and 20°C
Brugia malayi
5.4.2.12
0.38
-
3-phospho-D-glycerate
at pH 7.9 and 20°C
Caenorhabditis elegans
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.12
2-phospho-D-glycerate
Caenorhabditis elegans
-
3-phospho-D-glycerate
-
-
r
5.4.2.12
2-phospho-D-glycerate
Brugia malayi
-
3-phospho-D-glycerate
-
-
r
5.4.2.12
3-phospho-D-glycerate
Caenorhabditis elegans
-
2-phospho-D-glycerate
-
-
r
5.4.2.12
3-phospho-D-glycerate
Brugia malayi
-
2-phospho-D-glycerate
-
-
r
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.12
2-phospho-D-glycerate
-
749047
Caenorhabditis elegans
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
2-phospho-D-glycerate
-
749047
Brugia malayi
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
749047
Caenorhabditis elegans
2-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
749047
Brugia malayi
2-phospho-D-glycerate
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.4.2.12
?
x * 57000, SDS-PAGE
Brugia malayi
5.4.2.12
?
x * 59000, SDS-PAGE
Caenorhabditis elegans