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Literature summary extracted from

  • Gonzalez-Thuillier, I.; Venegas-Caleron, M.; Sanchez, R.; Garces, R.; von Wettstein-Knowles, P.; Martinez-Force, E.
    Sunflower (Helianthus annuus) fatty acid synthase complex beta-hydroxyacyl-[acyl carrier protein] dehydratase genes (2016), Planta, 243, 397-410 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.59 expression in Escherichia coli Helianthus annuus

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.59 F166V the HaHAD1 F166V mutant protein has a Km for crotonyl-CoA of 0.823 mM and the Vmax 0.02161 mM/min*mg, indicatating that the mutant protein is more active with crotonyl-CoA than wild-type HaHAD1 Helianthus annuus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.59 0.0685
-
 (3R)-3-hydroxybutyryl-[acyl-carrier protein] pH 6.8, temperature not specified in the publication, HaHAD1 wild-type protein Helianthus annuus
4.2.1.59 0.0823
-
 (3R)-3-hydroxybutyryl-[acyl-carrier protein] pH 6.8, temperature not specified in the publication, HaHAD1 F166V mutant Helianthus annuus
4.2.1.59 0.09773
-
 (3R)-3-hydroxybutyryl-[acyl-carrier protein] pH 6.8, temperature not specified in the publication, HaHAD2 wild-type protein Helianthus annuus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.59 Helianthus annuus F2VYC9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.59
-
 Helianthus annuus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.59 (3R)-3-hydroxybutyryl-[acyl-carrier protein]
-
 Helianthus annuus 2-butenoyl-[acyl-carrier protein] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.59 beta-hydroxyacyl-[ACP] dehydratase
-
 Helianthus annuus
4.2.1.59 HaHAD1
-
 Helianthus annuus
4.2.1.59 HaHAD2
-
 Helianthus annuus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.1.59 Helianthus annuus calculated from sequence
-
 5.67

Expression

EC Number Organism Comment Expression
4.2.1.59 Helianthus annuus higher levels of HaHAD2 in the initial stages of seed development infer its key role in seed storage fatty acid synthesis up

General Information

EC Number General Information Comment Organism
4.2.1.59 metabolism beta-hydroxyacyl-[acyl carrier protein (ACP)]-dehydratase is a component of the type II fatty acid synthase complex involved in de novo fatty acid biosynthesis in plants. Expression levels of HaHAD1 remains constant across most tissues suggest a housekeeping function Helianthus annuus
4.2.1.59 metabolism beta-hydroxyacyl-[acyl carrier protein (ACP)]-dehydratase is a component of the type II fatty acid synthase complex involved in de novo fatty acid biosynthesis in plants. Higher levels of HaHAD2 in the initial stages of seed development inferr its key role in seed storage fatty acid synthesis Helianthus annuus