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Literature summary extracted from

  • Ward, J.M.; Sze, H.
    Proton transport activity of the purified vacuolar H-ATPase from oats direct stimulation by Cl (1992), Plant Physiol., 99, 925-931 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.1.2.2 gramicidin D
-
 Avena sativa
7.1.2.2 soybean phospholipid
-
 Avena sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.2.2 bafilomycin A1 completely inhibited by less than 50 nM bafilomycin A1 Avena sativa
7.1.2.2 additional information not inhibited by azide or vanadate Avena sativa
7.1.2.2 N,N'-dicyclohexylcarbodiimide
-
 Avena sativa
7.1.2.2 NO3- 50 mM NO3- inhibits 90% of the hydrolytic activity, while H+ transport activity is decreased by 50% Avena sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.2.2 vacuolar membrane
-
 Avena sativa 5774
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.2.2 Cl- chloride stimulates both ATP hydrolysis by the purified ATPase and H+ pumping by the reconstituted ATPase in the presence of K+ and valinomycin Avena sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.2.2 650000
-
 gel filtration Avena sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.1.2.2 ATP + H2O + H+[side 1] Avena sativa
-
 ADP + phosphate + H+[side 2]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.1.2.2 Avena sativa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.2.2 Q-Sepharose column chromatography and Superdex 200 gel filtration Avena sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.1.2.2 root
-
 Avena sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.2.2 ATP + H2O + H+[side 1]
-
 Avena sativa ADP + phosphate + H+[side 2]
-
 ?

Subunits

EC Number Subunits Comment Organism
7.1.2.2 heterodecamer x * 70000 + x * 60000 + x * 44000 + x * 42000 + x * 36000 + x * 32000 + x * 29000 + x * 16000 + x * 13000 + x * 12000, SDS-PAGE Avena sativa

Synonyms

EC Number Synonyms Comment Organism
7.1.2.2 vacuolar H+-ATPase
-
 Avena sativa
7.1.2.2 vacuolar H+-pumping ATPase
-
 Avena sativa

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
7.1.2.2 0.000001
-
 at pH and °C Avena sativa bafilomycin A1