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Literature summary extracted from

  • Su, P.; Guan, H.; Zhao, Y.; Tong, Y.; Xu, M.; Zhang, Y.; Hu, T.; Yang, J.; Cheng, Q.; Gao, L.; Liu, Y.; Zhou, J.; Peters, R.J.; Huang, L.; Gao, W.
    Identification and functional characterization of diterpene synthases for triptolide biosynthesis from Tripterygium wilfordii (2018), Plant J., 93, 50-65 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.131 TwTPS27v2, phylogenetic analysis and tree, coexpression of TwTPS27v2 with either copalyl diphosphate synthases TwTPS7v2 or TwTPS9v2 in appropriately engineered Saccharomyces cerevisiae strain, or recombinant coexpression in Escherichia coli, the combination with TwTPS7v2 produces miltiradiene, recombinant expression oof GFP-tagged enzyme TwTPS27v2 in Nicotiana benthamiana chloroplasts, quantitative RT-PCR expression analysis Tripterygium wilfordii

Protein Variants

EC Number Protein Variants Comment Organism
4.2.3.131 additional information RNAi targeting of TwTPS27v2 is carried out reduces the transcript level of TwTPS27v2 to about 30%, with no reduction in the levels of the related TPS-b subfamily members TwTPS22v2, TwTPS23v2, TwTPS32 and TwTPS33, triptolide accumulation is decreased by about 40% Tripterygium wilfordii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.3.131 chloroplast
-
 Tripterygium wilfordii 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.131 (+)-copalyl diphosphate Tripterygium wilfordii
-
 miltiradiene + diphosphate
-
 ?
4.2.3.131 additional information Tripterygium wilfordii miltiradiene spontaneously forms abietatriene ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.131 Tripterygium wilfordii A0A1C7AAM8
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.131 cell suspension culture
-
 Tripterygium wilfordii
-
4.2.3.131 leaf transcriptome analysis Tripterygium wilfordii
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.131 (+)-copalyl diphosphate
-
 Tripterygium wilfordii miltiradiene + diphosphate
-
 ?
4.2.3.131 additional information miltiradiene spontaneously forms abietatriene Tripterygium wilfordii ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.131 TwTPS27v2
-
 Tripterygium wilfordii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.131 25
-
 assay at Tripterygium wilfordii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.131 7.2
-
 assay at Tripterygium wilfordii

General Information

EC Number General Information Comment Organism
4.2.3.131 evolution the enzyme TwTPS27v2 belongs to the terpene synthases superfamily and the TPS-b subfamily Tripterygium wilfordii
4.2.3.131 malfunction RNA interference targeting either both of the copalyl diphosphate synthases, TwTPS7v2 and TwTPS9v2, or the subsequently acting miltiradiene synthase, TwTPS27v2, leads to decreased production of triptolide Tripterygium wilfordii
4.2.3.131 metabolism miltiradiene is the relevant precursor in the biosynthesis of tripolide. Triptolide production is induced by methyl jasmonate. ENzyme TwTPS7v2, TwTPS9v2 and TwTPS27v2 are involved in triptolide biosynthesis Tripterygium wilfordii
4.2.3.131 physiological function miltiradiene is the relevant precursor in the biosynthesis of tripolide. Triptolide and celastrol are the main active diterpenoid and triterpenoid ingredients, respectively, that exhibit impressive and effective anti-inflammatory, immunosuppressive and anti-tumor activities and make Tripterygium wilfordii a medicinal plant Tripterygium wilfordii