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Literature summary extracted from

  • Hirato, Y.; Tokuhisa, M.; Tanigawa, M.; Ashida, H.; Tanaka, H.; Nishimura, K.
    Cloning and characterization of D-threonine aldolase from the green alga Chlamydomonas reinhardtii (2017), Phytochemistry, 135, 18-23 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.2.42 8-hydroxyquinoline 1 mM, 5% activation Chlamydomonas reinhardtii

Application

EC Number Application Comment Organism
4.1.2.42 synthesis the enzyme is a powerful tool for the stereospecific synthesis of various beta-hydroxy amino acids in synthetic organic chemistry Chlamydomonas reinhardtii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.42 overexpressed in recombinant Escherichia coli BL21 (DE3) cells Chlamydomonas reinhardtii

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.42 CaCl2 0.1 mM, 87% inhibition Chlamydomonas reinhardtii
4.1.2.42 CoCl2 0.1 mM, 12% inhibition Chlamydomonas reinhardtii
4.1.2.42 CuCl2 0.1 mM, complete inhibition Chlamydomonas reinhardtii
4.1.2.42 EDTA 1 mM, complete inhibition Chlamydomonas reinhardtii
4.1.2.42 hydroxylamine 1 mM, 95% inhibition Chlamydomonas reinhardtii
4.1.2.42 iodoacetate 1 mM, 11% inhibition Chlamydomonas reinhardtii
4.1.2.42 MgCl2 0.1 mM, 24% inhibition Chlamydomonas reinhardtii
4.1.2.42 NiCl2 0.1 mM, 16 % inhibition Chlamydomonas reinhardtii
4.1.2.42 p-chloromercuribenzoate 1 mM, 7% inhibition Chlamydomonas reinhardtii
4.1.2.42 Sodium cyanide 1 mM, 87% inhibition Chlamydomonas reinhardtii
4.1.2.42 ZnCl2 0.1 mM, complete inhibition Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.42 0.31
-
 D-threonine pH 8.1, 50°C Chlamydomonas reinhardtii
4.1.2.42 0.81
-
 D-threo-phenylserine pH 8.1, 50°C Chlamydomonas reinhardtii
4.1.2.42 2.4
-
 D-allo-threonine pH 8.1, 50°C Chlamydomonas reinhardtii

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.42 Chlamydomonas reinhardtii A0A1C9ZZ39
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.42
-
 Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.42 7.05
-
 pH 8.1, 50°C Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.42 D-allo-threonine
-
 Chlamydomonas reinhardtii glycine + acetaldehyde
-
 r
4.1.2.42 D-threo-phenylserine
-
 Chlamydomonas reinhardtii benzaldehyde + glycine
-
 r
4.1.2.42 D-threonine
-
 Chlamydomonas reinhardtii glycine + acetaldehyde
-
 r
4.1.2.42 glycine + acetaldehyde the enzyme catalyzes the synthesis of D- and D-allothreonine from a mixture of glycine and acetaldehyde. The diastereomer excess of D-threonine is 18% Chlamydomonas reinhardtii D-allothreonine
-
 r
4.1.2.42 glycine + acetaldehyde the enzyme catalyzes the synthesis of D- and D-allothreonine from a mixture of glycine and acetaldehyde. The diastereomer excess of D-threonine is 18% Chlamydomonas reinhardtii D-threonine
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.42 70
-
 cleavage of D-threonine Chlamydomonas reinhardtii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.42 50
-
 1 h, 90% of residual activity Chlamydomonas reinhardtii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.42 4.2
-
 D-threonine pH 8.1, 50°C Chlamydomonas reinhardtii
4.1.2.42 25
-
 D-allo-threonine pH 8.1, 50°C Chlamydomonas reinhardtii
4.1.2.42 280
-
 D-threo-phenylserine pH 8.1, 50°C Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.42 8.4
-
 cleavage of D-threonine, in 0.1 M HEPES-NaOH buffer Chlamydomonas reinhardtii

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.42 pyridoxal 5'-phosphate pyridoxal 5'-phosphate-dependent enzyme Chlamydomonas reinhardtii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.2.42 11
-
 D-allo-threonine pH 8.1, 50°C Chlamydomonas reinhardtii
4.1.2.42 13
-
 D-threonine pH 8.1, 50°C Chlamydomonas reinhardtii
4.1.2.42 350
-
 D-threo-phenylserine pH 8.1, 50°C Chlamydomonas reinhardtii