BRENDA - Enzyme Database

Molecular, biochemical characterization and assessment of immunogenic potential of cofactor-independent phosphoglycerate mutase against Leishmania donovani A step towards exploring novel vaccine candidate

Tandon, R.; Chandra, S.; Baharia, R.; Misra, P.; Das, S.; Rawat, K.; Siddiqi, M.; Sundar, S.; Dube, A.; Parasitology 145, 508-526 (2018)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
5.4.2.12
medicine
the enzyme is a potential vaccine candidate against visceral leishmaniasis
Leishmania donovani
Cloned(Commentary)
EC Number
Commentary
Organism
5.4.2.12
expressed in Escherichia coli BL21-Codon Plus (DE3) cells
Leishmania donovani
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.2.12
SPB00184
i.e. 2-hydroxyiminoindane-1,3-dione
Leishmania donovani
5.4.2.12
TL00638
i.e. 1-methyl-1H-imidazole-4,5-dicarboxylic acid
Leishmania donovani
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.2.12
0.143
-
2-phospho-D-glycerate
at pH 7.6 and 37C
Leishmania donovani
5.4.2.12
0.302
-
3-phospho-D-glycerate
at pH 7.6 and 37C
Leishmania donovani
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.4.2.12
cytoplasm
-
Leishmania donovani
5737
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.2.12
67
-
gel filtration
Leishmania donovani
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.12
2-phospho-D-glycerate
Leishmania donovani
-
3-phospho-D-glycerate
-
-
r
5.4.2.12
3-phospho-D-glycerate
Leishmania donovani
-
2-phospho-D-glycerate
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.2.12
Leishmania donovani
D7R810
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.4.2.12
Ni-NTA agarose column chromatography
Leishmania donovani
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.12
2-phospho-D-glycerate
-
748834
Leishmania donovani
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
748834
Leishmania donovani
2-phospho-D-glycerate
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
5.4.2.12
monomer
1 * 60000, SDS-PAGE
Leishmania donovani
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4.2.12
7.5
8
-
Leishmania donovani
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.12
additional information
the enzyme is independent from 2,3-bisphosphoglycerate
Leishmania donovani
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
5.4.2.12
0.0373
-
at pH 7.6 and 37C
Leishmania donovani
TL00638
5.4.2.12
0.0609
-
at pH 7.6 and 37C
Leishmania donovani
SPB00184
Application (protein specific)
EC Number
Application
Commentary
Organism
5.4.2.12
medicine
the enzyme is a potential vaccine candidate against visceral leishmaniasis
Leishmania donovani
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.12
expressed in Escherichia coli BL21-Codon Plus (DE3) cells
Leishmania donovani
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.12
additional information
the enzyme is independent from 2,3-bisphosphoglycerate
Leishmania donovani
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
5.4.2.12
0.0373
-
at pH 7.6 and 37C
Leishmania donovani
TL00638
5.4.2.12
0.0609
-
at pH 7.6 and 37C
Leishmania donovani
SPB00184
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.2.12
SPB00184
i.e. 2-hydroxyiminoindane-1,3-dione
Leishmania donovani
5.4.2.12
TL00638
i.e. 1-methyl-1H-imidazole-4,5-dicarboxylic acid
Leishmania donovani
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.2.12
0.143
-
2-phospho-D-glycerate
at pH 7.6 and 37C
Leishmania donovani
5.4.2.12
0.302
-
3-phospho-D-glycerate
at pH 7.6 and 37C
Leishmania donovani
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.4.2.12
cytoplasm
-
Leishmania donovani
5737
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.2.12
67
-
gel filtration
Leishmania donovani
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.12
2-phospho-D-glycerate
Leishmania donovani
-
3-phospho-D-glycerate
-
-
r
5.4.2.12
3-phospho-D-glycerate
Leishmania donovani
-
2-phospho-D-glycerate
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.12
Ni-NTA agarose column chromatography
Leishmania donovani
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.12
2-phospho-D-glycerate
-
748834
Leishmania donovani
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
748834
Leishmania donovani
2-phospho-D-glycerate
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.4.2.12
monomer
1 * 60000, SDS-PAGE
Leishmania donovani
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4.2.12
7.5
8
-
Leishmania donovani