BRENDA - Enzyme Database

Co-factor-independent phosphoglycerate mutase of Leishmania donovani modulates macrophage signalling and promotes T-cell repertoires bearing epitopes for both MHC-I and MHC-II

Singh, M.; Jamal, F.; Dubey, A.; Shivam, P.; Kumari, S.; Pushpanjal, P.; Ahmed, G.; Dikhit, M.; Narayan, S.; Das, V.; Pandey, K.; Sinha, K.; Das, P.; Singh, S.; Parasitology 145, 292-306 (2018)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.4.2.12
expressed in Escherichia coli BLR(DE3) cells
Leishmania donovani
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.4.2.12
cytosol
-
Leishmania donovani
5829
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.12
2-phospho-D-glycerate
Leishmania donovani
-
3-phospho-D-glycerate
-
-
r
5.4.2.12
3-phospho-D-glycerate
Leishmania donovani
-
2-phospho-D-glycerate
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.2.12
Leishmania donovani
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.4.2.12
Ni-NTA bead chromatography
Leishmania donovani
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.12
2-phospho-D-glycerate
-
748833
Leishmania donovani
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
748833
Leishmania donovani
2-phospho-D-glycerate
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
5.4.2.12
monomer
1 * 61000, SDS-PAGE
Leishmania donovani
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.12
additional information
the enzyme is independent from 2,3-bisphosphoglycerate
Leishmania donovani
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.12
expressed in Escherichia coli BLR(DE3) cells
Leishmania donovani
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.12
additional information
the enzyme is independent from 2,3-bisphosphoglycerate
Leishmania donovani
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.4.2.12
cytosol
-
Leishmania donovani
5829
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.12
2-phospho-D-glycerate
Leishmania donovani
-
3-phospho-D-glycerate
-
-
r
5.4.2.12
3-phospho-D-glycerate
Leishmania donovani
-
2-phospho-D-glycerate
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.12
Ni-NTA bead chromatography
Leishmania donovani
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.12
2-phospho-D-glycerate
-
748833
Leishmania donovani
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
748833
Leishmania donovani
2-phospho-D-glycerate
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.4.2.12
monomer
1 * 61000, SDS-PAGE
Leishmania donovani
General Information
EC Number
General Information
Commentary
Organism
5.4.2.12
physiological function
the enzyme modulates macrophage signaling and promotes T-cell repertoires bearing epitopes for both major histocompatibility complex classes I and II. Enzyme stimulation induces higher expression of interleukin (IL)-1beta in the phagocytic cell, its receptor and CD69 on T-cell subsets. These cellular activations result in upregulation of host-protective cytokines IL-2, IL-12, IL-17, tumour necrosis factor-alpha and interferon-gamma and downregulation of IL-4, IL-10 and tumour growth factor-beta. Enzyme stimulation also promotes lymphocyte proliferation and boosted the leishmaniacidal activity of macrophages by upregulating reactive oxygen species. It also induces 1.8fold higher release of nitric oxide by promoting the transcription of inducible nitric oxide synthase gene
Leishmania donovani
General Information (protein specific)
EC Number
General Information
Commentary
Organism
5.4.2.12
physiological function
the enzyme modulates macrophage signaling and promotes T-cell repertoires bearing epitopes for both major histocompatibility complex classes I and II. Enzyme stimulation induces higher expression of interleukin (IL)-1beta in the phagocytic cell, its receptor and CD69 on T-cell subsets. These cellular activations result in upregulation of host-protective cytokines IL-2, IL-12, IL-17, tumour necrosis factor-alpha and interferon-gamma and downregulation of IL-4, IL-10 and tumour growth factor-beta. Enzyme stimulation also promotes lymphocyte proliferation and boosted the leishmaniacidal activity of macrophages by upregulating reactive oxygen species. It also induces 1.8fold higher release of nitric oxide by promoting the transcription of inducible nitric oxide synthase gene
Leishmania donovani