EC Number | Cloned (Comment) | Organism |
---|---|---|
5.4.99.B40 | gene bra032185, DNA and amino acid sequence analysis, sequence comparisons, and phylogenetic analysis | Brassica rapa subsp. pekinensis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.B40 | (3S)-2,3-epoxy-2,3-dihydrosqualene | Brassica rapa subsp. pekinensis | - |
astertarone A | - |
? | |
5.4.99.B40 | additional information | Brassica rapa subsp. pekinensis | the Brassica rapa oxidosqualene cyclase Bra032185 produces 60% astertarone A and 20 minor triterpenes (0.1-11%), i.e. butyrospermol (11.4%), euphol (8.6%), dammar-21-ene-3beta,18(S)-diol (6.7%), boeticol (4.2%), dammar-21-ene-3beta,18(R)-diol (1.8%), isoeuphol (1.4%), dammar-18(28)-21-dien-3-beta-ol (1.2%), camelliol C (1.0%), and below 1% of isohelianol, 9alphaH-polypoda-7,13E,17E,21-tetraenol, isotirucallol, (20R)--dammara-12,21-dien-3beta-ol, 9alphaH-polypoda-8(26),13E,17E,21-tetraenol, 14-epiarabindiol, (18S)-dammara-12,21-dien-3beta-ol, dammara-18(19)Z-21-dien-3beta-ol, and dammara-18(19)E,21-dien-3-beta-ol | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.4.99.B40 | Brassica rapa subsp. pekinensis | M4ETQ3 | - |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
5.4.99.B40 | (3S)-2,3-epoxy-2,3-dihydrosqualene = astertarone A | reaction mechanism via intermediate forms dammarenyl cation, C-4 cation, and enol form, overview | Brassica rapa subsp. pekinensis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.B40 | (3S)-2,3-epoxy-2,3-dihydrosqualene | - |
Brassica rapa subsp. pekinensis | astertarone A | - |
? | |
5.4.99.B40 | additional information | the Brassica rapa oxidosqualene cyclase Bra032185 produces 60% astertarone A and 20 minor triterpenes (0.1-11%), i.e. butyrospermol (11.4%), euphol (8.6%), dammar-21-ene-3beta,18(S)-diol (6.7%), boeticol (4.2%), dammar-21-ene-3beta,18(R)-diol (1.8%), isoeuphol (1.4%), dammar-18(28)-21-dien-3-beta-ol (1.2%), camelliol C (1.0%), and below 1% of isohelianol, 9alphaH-polypoda-7,13E,17E,21-tetraenol, isotirucallol, (20R)--dammara-12,21-dien-3beta-ol, 9alphaH-polypoda-8(26),13E,17E,21-tetraenol, 14-epiarabindiol, (18S)-dammara-12,21-dien-3beta-ol, dammara-18(19)Z-21-dien-3beta-ol, and dammara-18(19)E,21-dien-3-beta-ol | Brassica rapa subsp. pekinensis | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.4.99.B40 | Bra032185 | - |
Brassica rapa subsp. pekinensis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
5.4.99.B40 | evolution | the enzyme is a terpene cyclase/mutase family member, phylogenetic tree | Brassica rapa subsp. pekinensis |
5.4.99.B40 | additional information | comparison of active-site residues | Brassica rapa subsp. pekinensis |