BRENDA - Enzyme Database

Chemical differentiation of three DMSP lyases from the marine Roseobacter group

Burkhardt, I.; Lauterbach, L.; Brock, N.; Dickschat, J.; Org. Biomol. Chem. 15, 4432-4439 (2017)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.4.1.3
4.2
-
dimethylseleniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
5.7
-
3-(thiolan-1-ium-1-yl)propanoate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
8
-
methylpropylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
8.2
-
diethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
10.7
-
ethylmethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
12.8
-
dimethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
16.4
-
diethylsulfoniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
18.7
-
dimethylseleniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
21.4
-
dimethylseleniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
26.1
-
ethylmethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
27.8
-
methylpropylsulfoniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
28.6
-
dimethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
31.4
-
3-(thiolan-1-ium-1-yl)propanoate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
31.4
-
diethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
33.4
-
ethylmethylsulfoniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
34.8
-
dimethylsulfoniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
35.7
-
methylpropylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
102.6
-
3-(thiolan-1-ium-1-yl)propanoate
30°C, pH not specified in the publication
Phaeobacter inhibens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.4.1.3
Phaeobacter inhibens
-
-
-
4.4.1.3
Phaeobacter inhibens DSM 17395
-
-
-
4.4.1.3
Ruegeria pomeroyi
Q5LT18
-
-
4.4.1.3
Ruegeria pomeroyi
Q5LW89
-
-
4.4.1.3
Ruegeria pomeroyi DSM 15171
Q5LT18
-
-
4.4.1.3
Ruegeria pomeroyi DSM 15171
Q5LW89
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.4.1.3
3-(thiolan-1-ium-1-yl)propanoate
-
748814
Phaeobacter inhibens
thiolane + acrylate
-
-
-
?
4.4.1.3
3-(thiolan-1-ium-1-yl)propanoate
-
748814
Ruegeria pomeroyi
thiolane + acrylate
-
-
-
?
4.4.1.3
3-(thiolan-1-ium-1-yl)propanoate
-
748814
Ruegeria pomeroyi DSM 15171
thiolane + acrylate
-
-
-
?
4.4.1.3
3-(thiolan-1-ium-1-yl)propanoate
-
748814
Phaeobacter inhibens DSM 17395
thiolane + acrylate
-
-
-
?
4.4.1.3
diethylsulfoniopropionate
-
748814
Phaeobacter inhibens
diethylsulfide + acrylate
-
-
-
?
4.4.1.3
diethylsulfoniopropionate
-
748814
Ruegeria pomeroyi
diethylsulfide + acrylate
-
-
-
?
4.4.1.3
diethylsulfoniopropionate
-
748814
Ruegeria pomeroyi DSM 15171
diethylsulfide + acrylate
-
-
-
?
4.4.1.3
diethylsulfoniopropionate
-
748814
Phaeobacter inhibens DSM 17395
diethylsulfide + acrylate
-
-
-
?
4.4.1.3
dimethylseleniopropionate
-
748814
Phaeobacter inhibens
dimethylselenide + acrylate
-
-
-
?
4.4.1.3
dimethylseleniopropionate
-
748814
Ruegeria pomeroyi
dimethylselenide + acrylate
-
-
-
?
4.4.1.3
dimethylseleniopropionate
-
748814
Ruegeria pomeroyi DSM 15171
dimethylselenide + acrylate
-
-
-
?
4.4.1.3
dimethylseleniopropionate
-
748814
Phaeobacter inhibens DSM 17395
dimethylselenide + acrylate
-
-
-
?
4.4.1.3
dimethylsulfoniopropionate
-
748814
Phaeobacter inhibens
dimethylsulfide + acrylate
-
-
-
?
4.4.1.3
dimethylsulfoniopropionate
-
748814
Ruegeria pomeroyi
dimethylsulfide + acrylate
-
-
-
?
4.4.1.3
dimethylsulfoniopropionate
-
748814
Ruegeria pomeroyi DSM 15171
dimethylsulfide + acrylate
-
-
-
?
4.4.1.3
dimethylsulfoniopropionate
-
748814
Phaeobacter inhibens DSM 17395
dimethylsulfide + acrylate
-
-
-
?
4.4.1.3
ethylmethylsulfoniopropionate
-
748814
Phaeobacter inhibens
ethylmethylsulfide + acrylate
-
-
-
?
4.4.1.3
ethylmethylsulfoniopropionate
-
748814
Ruegeria pomeroyi
ethylmethylsulfide + acrylate
-
-
-
?
4.4.1.3
ethylmethylsulfoniopropionate
-
748814
Ruegeria pomeroyi DSM 15171
ethylmethylsulfide + acrylate
-
-
-
?
4.4.1.3
ethylmethylsulfoniopropionate
-
748814
Phaeobacter inhibens DSM 17395
ethylmethylsulfide + acrylate
-
-
-
?
4.4.1.3
methylpropylsulfoniopropionate
-
748814
Phaeobacter inhibens
methylpropylsulfide + acrylate
-
-
-
?
4.4.1.3
methylpropylsulfoniopropionate
-
748814
Ruegeria pomeroyi
methylpropylsulfide + acrylate
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.4.1.3
0.93
-
dimethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
1.47
-
ethylmethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
1.58
-
3-(thiolan-1-ium-1-yl)propanoate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
1.67
-
methylpropylsulfoniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
1.71
-
diethylsulfoniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
1.83
-
dimethylseleniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
2.04
-
3-(thiolan-1-ium-1-yl)propanoate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
2.07
-
ethylmethylsulfoniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
2.22
-
diethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
3.73
-
methylpropylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
3.85
-
dimethylsulfoniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
4.25
-
dimethylseleniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
10.9
-
methylpropylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
11.5
-
ethylmethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
11.9
-
diethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
16.8
-
dimethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
21.9
-
3-(thiolan-1-ium-1-yl)propanoate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
31.2
-
dimethylseleniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.4.1.3
4.2
-
dimethylseleniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
5.7
-
3-(thiolan-1-ium-1-yl)propanoate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
8
-
methylpropylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
8.2
-
diethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
10.7
-
ethylmethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
12.8
-
dimethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
16.4
-
diethylsulfoniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
18.7
-
dimethylseleniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
21.4
-
dimethylseleniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
26.1
-
ethylmethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
27.8
-
methylpropylsulfoniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
28.6
-
dimethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
31.4
-
3-(thiolan-1-ium-1-yl)propanoate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
31.4
-
diethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
33.4
-
ethylmethylsulfoniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
34.8
-
dimethylsulfoniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
35.7
-
methylpropylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
102.6
-
3-(thiolan-1-ium-1-yl)propanoate
30°C, pH not specified in the publication
Phaeobacter inhibens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.4.1.3
3-(thiolan-1-ium-1-yl)propanoate
-
748814
Phaeobacter inhibens
thiolane + acrylate
-
-
-
?
4.4.1.3
3-(thiolan-1-ium-1-yl)propanoate
-
748814
Ruegeria pomeroyi
thiolane + acrylate
-
-
-
?
4.4.1.3
3-(thiolan-1-ium-1-yl)propanoate
-
748814
Ruegeria pomeroyi DSM 15171
thiolane + acrylate
-
-
-
?
4.4.1.3
3-(thiolan-1-ium-1-yl)propanoate
-
748814
Phaeobacter inhibens DSM 17395
thiolane + acrylate
-
-
-
?
4.4.1.3
diethylsulfoniopropionate
-
748814
Phaeobacter inhibens
diethylsulfide + acrylate
-
-
-
?
4.4.1.3
diethylsulfoniopropionate
-
748814
Ruegeria pomeroyi
diethylsulfide + acrylate
-
-
-
?
4.4.1.3
diethylsulfoniopropionate
-
748814
Ruegeria pomeroyi DSM 15171
diethylsulfide + acrylate
-
-
-
?
4.4.1.3
diethylsulfoniopropionate
-
748814
Phaeobacter inhibens DSM 17395
diethylsulfide + acrylate
-
-
-
?
4.4.1.3
dimethylseleniopropionate
-
748814
Phaeobacter inhibens
dimethylselenide + acrylate
-
-
-
?
4.4.1.3
dimethylseleniopropionate
-
748814
Ruegeria pomeroyi
dimethylselenide + acrylate
-
-
-
?
4.4.1.3
dimethylseleniopropionate
-
748814
Ruegeria pomeroyi DSM 15171
dimethylselenide + acrylate
-
-
-
?
4.4.1.3
dimethylseleniopropionate
-
748814
Phaeobacter inhibens DSM 17395
dimethylselenide + acrylate
-
-
-
?
4.4.1.3
dimethylsulfoniopropionate
-
748814
Phaeobacter inhibens
dimethylsulfide + acrylate
-
-
-
?
4.4.1.3
dimethylsulfoniopropionate
-
748814
Ruegeria pomeroyi
dimethylsulfide + acrylate
-
-
-
?
4.4.1.3
dimethylsulfoniopropionate
-
748814
Ruegeria pomeroyi DSM 15171
dimethylsulfide + acrylate
-
-
-
?
4.4.1.3
dimethylsulfoniopropionate
-
748814
Phaeobacter inhibens DSM 17395
dimethylsulfide + acrylate
-
-
-
?
4.4.1.3
ethylmethylsulfoniopropionate
-
748814
Phaeobacter inhibens
ethylmethylsulfide + acrylate
-
-
-
?
4.4.1.3
ethylmethylsulfoniopropionate
-
748814
Ruegeria pomeroyi
ethylmethylsulfide + acrylate
-
-
-
?
4.4.1.3
ethylmethylsulfoniopropionate
-
748814
Ruegeria pomeroyi DSM 15171
ethylmethylsulfide + acrylate
-
-
-
?
4.4.1.3
ethylmethylsulfoniopropionate
-
748814
Phaeobacter inhibens DSM 17395
ethylmethylsulfide + acrylate
-
-
-
?
4.4.1.3
methylpropylsulfoniopropionate
-
748814
Phaeobacter inhibens
methylpropylsulfide + acrylate
-
-
-
?
4.4.1.3
methylpropylsulfoniopropionate
-
748814
Ruegeria pomeroyi
methylpropylsulfide + acrylate
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.4.1.3
0.93
-
dimethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
1.47
-
ethylmethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
1.58
-
3-(thiolan-1-ium-1-yl)propanoate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
1.67
-
methylpropylsulfoniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
1.71
-
diethylsulfoniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
1.83
-
dimethylseleniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
2.04
-
3-(thiolan-1-ium-1-yl)propanoate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
2.07
-
ethylmethylsulfoniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
2.22
-
diethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
3.73
-
methylpropylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
3.85
-
dimethylsulfoniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
4.25
-
dimethylseleniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
10.9
-
methylpropylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
11.5
-
ethylmethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
11.9
-
diethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
16.8
-
dimethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
21.9
-
3-(thiolan-1-ium-1-yl)propanoate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
31.2
-
dimethylseleniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.4.1.3
0.02
-
3-(thiolan-1-ium-1-yl)propanoate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
0.032
-
dimethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
0.05
-
3-(thiolan-1-ium-1-yl)propanoate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
0.056
-
ethylmethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
0.06
-
methylpropylsulfoniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
0.062
-
ethylmethylsulfoniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
0.071
-
diethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
0.098
-
dimethylseleniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
0.104
-
diethylsulfoniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
0.105
-
methylpropylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
0.111
-
dimethylsulfoniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
0.199
-
dimethylseleniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
1.08
-
ethylmethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
1.32
-
dimethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
1.35
-
methylpropylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
1.46
-
diethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
3.82
-
3-(thiolan-1-ium-1-yl)propanoate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
7.38
-
dimethylseleniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.4.1.3
0.02
-
3-(thiolan-1-ium-1-yl)propanoate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
0.032
-
dimethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
0.05
-
3-(thiolan-1-ium-1-yl)propanoate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
0.056
-
ethylmethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
0.06
-
methylpropylsulfoniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
0.062
-
ethylmethylsulfoniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
0.071
-
diethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
0.098
-
dimethylseleniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
0.104
-
diethylsulfoniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
0.105
-
methylpropylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
0.111
-
dimethylsulfoniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
0.199
-
dimethylseleniopropionate
30°C, pH not specified in the publication
Phaeobacter inhibens
4.4.1.3
1.08
-
ethylmethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
1.32
-
dimethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
1.35
-
methylpropylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
1.46
-
diethylsulfoniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
3.82
-
3-(thiolan-1-ium-1-yl)propanoate
30°C, pH not specified in the publication
Ruegeria pomeroyi
4.4.1.3
7.38
-
dimethylseleniopropionate
30°C, pH not specified in the publication
Ruegeria pomeroyi