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Literature summary extracted from

  • Chauleau, M.; Shuman, S.
    Kinetic mechanism and fidelity of nick sealing by Escherichia coli NAD+-dependent DNA ligase (LigA) (2016), Nucleic Acids Res., 44, 2298-2309 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.2 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.2 NH4+ stimulates enzyme activity at 10 mM Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.2 NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m Escherichia coli
-
 (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.2 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.2 Ni-agarose column chromatography, and Superdex 200 gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.2 additional information the enzyme is relatively tolerant of 5'-phosphate base mispairs and 5' N:abasic lesions Escherichia coli ?
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m
-
 Escherichia coli (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.2 LigA
-
 Escherichia coli
6.5.1.2 NAD+-dependent DNA ligase
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.5.1.2 NAD+
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
6.5.1.2 physiological function the enzyme repairs 3'-OH/5'-PO4 nicks in duplex DNA via reaction with NAD+ to form a covalent enzyme-(lysyl-Nzeta)-AMP intermediate, transfer of AMP to the nick 5'-PO4 to form an AppDNA intermediate (step 2), and attack of the nick 3'-OH on AppDNA to form a 3'-5' phosphodiester Escherichia coli