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Literature summary extracted from

  • Peach, S.E.; York, K.; Hesselberth, J.R.
    Global analysis of RNA cleavage by 5-hydroxyl RNA sequencing (2015), Nucleic Acids Res., 43, e108 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.8 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.8 (ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O Escherichia coli
-
 (ribonucleotide)n+m + GMP + diphosphate
-
 ?
6.5.1.8 (ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + GTP Escherichia coli
-
 (ribonucleotide)n+m + GMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.8 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.8 Ni-NTA column chromatography, and HiTrap heparin column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.8 (ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O
-
 Escherichia coli (ribonucleotide)n+m + GMP + diphosphate
-
 ?
6.5.1.8 (ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + GTP
-
 Escherichia coli (ribonucleotide)n+m + GMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.5.1.8 ? x * 50000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
6.5.1.8 RtcB RNA ligase
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
6.5.1.8 physiological function the enzyme operates during the unfolded protein response Escherichia coli