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Literature summary extracted from

  • Paul, R.; Lazarevand, D.; Altman, S.
    Characterization of RNase P from Thermotoga maritima (2001), Nucleic Acids Res., 29, 880-885 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.26.5 expression of the protein subunit in Escherichia coli. The cloned protein is reconstituted with the RNA subunit transcribed in vitro Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.26.5 0.000223
-
 tRNATyr precursor pH 7, 50°C Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.5 KCl optimal concentration: 200 mM Thermotoga maritima
3.1.26.5 MgCl2 optimal activity at 5 mM Thermotoga maritima
3.1.26.5 NaCl optimal concentration: 50 mM Thermotoga maritima
3.1.26.5 NH4Cl optimal concentration: 200-400 mM Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.5 Thermotoga maritima
-
-
-
3.1.26.5 Thermotoga maritima DSM 3109
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.26.5
-
 Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.5 tRNATyr precursor + H2O
-
 Thermotoga maritima mature tRNATyr + 5'-terminal RNA oligonucleotide
-
 ?
3.1.26.5 tRNATyr precursor + H2O
-
 Thermotoga maritima DSM 3109 mature tRNATyr + 5'-terminal RNA oligonucleotide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.26.5 RNase P
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.26.5 50
-
-
 Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.26.5 40 55 40°C: about 45% of maximal activity, 55°C: about 25% of maximal activity Thermotoga maritima
3.1.26.5 65
-
 no activity at 65°C or above Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.26.5 0.137
-
 tRNATyr precursor pH 7, 50°C Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.26.5 7.5
-
-
 Thermotoga maritima