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Literature summary extracted from

  • Palatinszky, M.; Herbold, C.; Jehmlich, N.; Pogoda, M.; Han, P.; von Bergen, M.; Lagkouvardos, I.; Karst, S.M.; Galushko, A.; Koch, H.; Berry, D.; Daims, H.; Wagner, M.
    Cyanate as an energy source for nitrifiers (2015), Nature, 524, 105-108 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.104 gene cynS, DNA and amino acid sequence determination and analysis Candidatus Nitrososphaera gargensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.104 additional information the culture of Nitrososphaera gargensis grows well in the presence of 2 mM ammonium and growth is not inhibited by addition of 0.5 mM cyanate Candidatus Nitrososphaera gargensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.104 cyanate + HCO3- + 2 H+ Candidatus Nitrososphaera gargensis
-
 NH3 + 2 CO2
-
 ?
4.2.1.104 cyanate + HCO3- + 2 H+ Nitrospira moscoviensis
-
 NH3 + 2 CO2
-
 ?
4.2.1.104 cyanate + HCO3- + 2 H+ Candidatus Nitrososphaera gargensis Ga9.2
-
 NH3 + 2 CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.104 Candidatus Nitrososphaera gargensis K0INW1
-
-
4.2.1.104 Candidatus Nitrososphaera gargensis Ga9.2 K0INW1
-
-
4.2.1.104 Nitrospira moscoviensis A0A0K2GAS4
-
-
4.2.1.104 no activity in Nitrosomonas nitrosa strain Nm90
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.104 additional information after 96 h of incubation in the presence of around 1 mM cyanate Nitrospira moscoviensis degrades significantly more cyanate leading to ammonium release from the cells Nitrospira moscoviensis
-
4.2.1.104 additional information the culture of Nitrososphaera gargensis grows well in the presence of 2 mM ammonium and growth is not inhibited by addition of 0.5 mM cyanate. The cells can grow on cyanate as the only source of energy, reductant and nitrogen. The cells stochiometrically convert cyanate via ammonium to nitrite, and cyanate degradation is the rate-limiting step of the overall process Candidatus Nitrososphaera gargensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.104 cyanate + HCO3- + 2 H+
-
 Candidatus Nitrososphaera gargensis NH3 + 2 CO2
-
 ?
4.2.1.104 cyanate + HCO3- + 2 H+
-
 Nitrospira moscoviensis NH3 + 2 CO2
-
 ?
4.2.1.104 cyanate + HCO3- + 2 H+
-
 Candidatus Nitrososphaera gargensis Ga9.2 NH3 + 2 CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.104 cyanate hydrolase UniProt Nitrospira moscoviensis
4.2.1.104 CynS
-
 Candidatus Nitrososphaera gargensis
4.2.1.104 CynS
-
 Nitrospira moscoviensis
4.2.1.104 Ngar_c31480
-
 Candidatus Nitrososphaera gargensis
4.2.1.104 NITMOv2_1274
-
 Nitrospira moscoviensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.104 37
-
 in vivo assay at Candidatus Nitrososphaera gargensis
4.2.1.104 37
-
 in vivo assay at Nitrospira moscoviensis

General Information

EC Number General Information Comment Organism
4.2.1.104 evolution phylogenetic relationship of Nitrososphaera gargensis cyanase with the Nitrospira cyanases, overview Nitrospira moscoviensis
4.2.1.104 evolution while Nitrososphaera gargensis is the only ammonia-oxidizing microbe with a sequenced genome in which a cyanase is present that is likely acquired from a Nitrospira strain via lateral gene transfer, all nitrite-oxidizers for which a genome sequence is available contain a gene annotated as cyanase. Phylogenetic relationship of Nitrososphaera gargensis cyanase with the Nitrospira cyanases, overview Candidatus Nitrososphaera gargensis
4.2.1.104 physiological function Nitrospira moscoviensis cells are capable of cyanate degradation in vivo Nitrospira moscoviensis