Literature summary extracted from

Chen, X.; Pu, H.; Fang, Y.; Wang, X.; Zhao, S.; Lin, Y.; Zhang, M.; Dai, H.E.; Gong, W.; Liu, L.
Crystal structure of the catalytic subunit of magnesium chelatase (2015), Nat. Plants, 1, 15125 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
6.6.1.1	expressed in Escherichia coli BL21(DE3) cells	Synechocystis sp. PCC 6803

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
6.6.1.1	vapor diffusion method, using 0.85 M sodium citrate, pH 7.0 and 2 mM dithiothreitol	Synechocystis sp. PCC 6803

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
6.6.1.1	ATP + protoporphyrin IX + Mg2+ + H2O	Synechocystis sp. PCC 6803	-	ADP + phosphate + Mg-protoporphyrin IX + 2 H+	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
6.6.1.1	Synechocystis sp. PCC 6803	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
6.6.1.1	Ni-NTA column chromatography and Superdex 200 gel filtration	Synechocystis sp. PCC 6803

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6.6.1.1	ATP + protoporphyrin IX + Mg2+ + H2O	-	Synechocystis sp. PCC 6803	ADP + phosphate + Mg-protoporphyrin IX + 2 H+	-	?

Subunits

EC Number	Subunits	Comment	Organism
6.6.1.1	homodimer	2 * 150000, subunit ChlH, SDS-PAGE	Synechocystis sp. PCC 6803

Synonyms

EC Number	Synonyms	Comment	Organism
6.6.1.1	ChlH	catalytic subunit	Synechocystis sp. PCC 6803
6.6.1.1	MGCH	-	Synechocystis sp. PCC 6803

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
6.6.1.1	ATP	-	Synechocystis sp. PCC 6803

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Release 2023.1 (January 2023)