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Literature summary extracted from

  • Hammer, S.C.; Marjanovic, A.; Dominicus, J.M.; Nestl, B.M.; Hauer, B.
    Squalene hopene cyclases are protonases for stereoselective Bronsted acid catalysis (2015), Nat. Chem. Biol., 11, 121-126 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.17 A419F inactive Alicyclobacillus acidocaldarius
5.4.99.17 D376C inactive Alicyclobacillus acidocaldarius
5.4.99.17 F365A the mutant shows increased activity with geraniol and (S)-6,7-epoxygeraniol compared to the wild type enzyme Alicyclobacillus acidocaldarius
5.4.99.17 F365C the mutant exhibits 15% conversion of geraniol to gamma-cyclogeraniol Alicyclobacillus acidocaldarius
5.4.99.17 F365C the mutant shows increased activity with geraniol compared to the wild type enzyme Alicyclobacillus acidocaldarius
5.4.99.17 F365G the mutant shows increased activity with (S)-6,7-epoxygeraniol and no activity with geraniol compared to the wild type enzyme Alicyclobacillus acidocaldarius
5.4.99.17 F365W inactive Alicyclobacillus acidocaldarius
5.4.99.17 F601A the mutant shows about wild type activity Alicyclobacillus acidocaldarius
5.4.99.17 F601W the mutant shows about wild type activity Alicyclobacillus acidocaldarius
5.4.99.17 G600F the mutant exhibits 68% conversion of geraniol to cyclogeraniol hydrate Alicyclobacillus acidocaldarius
5.4.99.17 I261A the mutant exhibits 11% conversion of citronellal to (-)-iso-isopulegol. The mutant shows increased activity with geraniol and (S)-6,7-epoxygeraniol compared to the wild type enzyme Alicyclobacillus acidocaldarius
5.4.99.17 I261W the mutant shows increased activity with (S)-6,7-epoxygeraniol and no activity with geraniol compared to the wild type enzyme Alicyclobacillus acidocaldarius
5.4.99.17 L36A the mutant shows increased activity with (S)-6,7-epoxygeraniol compared to the wild type enzyme Alicyclobacillus acidocaldarius
5.4.99.17 L36W inactive Alicyclobacillus acidocaldarius
5.4.99.17 S307A the mutant shows increased activity with (S)-6,7-epoxygeraniol and no activity with geraniol compared to the wild type enzyme Alicyclobacillus acidocaldarius
5.4.99.17 S307F inactive Alicyclobacillus acidocaldarius
5.4.99.17 V448F inactive Alicyclobacillus acidocaldarius
5.4.99.17 W169A the mutant shows about wild type activity Alicyclobacillus acidocaldarius
5.4.99.17 W312A inactive Alicyclobacillus acidocaldarius
5.4.99.17 W489A inactive Alicyclobacillus acidocaldarius
5.4.99.17 Y420A inactive Alicyclobacillus acidocaldarius
5.4.99.17 Y420C the mutant shows about wild type activity Alicyclobacillus acidocaldarius
5.4.99.17 Y420W the mutant exhibits 54% conversion of (S)-6,7-epoxygeraniol to (1S,3R)-3-(hydroxymethyl)-2,2-dimethyl-4-methylidenecyclohexan-1-ol Alicyclobacillus acidocaldarius
5.4.99.17 Y420W/G600F the mutant exhibits 78% conversion of (S)-6,7-epoxygeraniol to (1S,3R)-3-(hydroxymethyl)-2,2-dimethyl-4-methylidenecyclohexan-1-ol Alicyclobacillus acidocaldarius
5.4.99.17 Y609W inactive Alicyclobacillus acidocaldarius

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.17 squalene Alicyclobacillus acidocaldarius
-
 hop-22(29)-ene
-
 ?
5.4.99.17 squalene + H2O Alicyclobacillus acidocaldarius
-
 hopan-22-ol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.17 Alicyclobacillus acidocaldarius P33247
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.17 (S)-6,7-epoxygeraniol + H2O 0.57% conversion with high enzyme concentrations Alicyclobacillus acidocaldarius (1S,3R)-3-(hydroxymethyl)-2,2-dimethyl-4-methylidenecyclohexan-1-ol
-
 ?
5.4.99.17 (S)-citronellal + H2O 0.59% conversion with high enzyme concentrations Alicyclobacillus acidocaldarius (-)-iso-isopulegol
-
 ?
5.4.99.17 geraniol + H2O 0.4% conversion with high enzyme concentrations Alicyclobacillus acidocaldarius gamma-cyclogeraniol + cyclogeraniol hydrate
-
 ?
5.4.99.17 squalene
-
 Alicyclobacillus acidocaldarius hop-22(29)-ene
-
 ?
5.4.99.17 squalene + H2O
-
 Alicyclobacillus acidocaldarius hopan-22-ol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.4.99.17 SHC
-
 Alicyclobacillus acidocaldarius
5.4.99.17 squalene hopene cyclase
-
 Alicyclobacillus acidocaldarius