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Literature summary extracted from

  • de Sousa, M.; Manzo, R.M.; Garcia, J.L.; Mammarella, E.J.; Goncalves, L.R.B.; Pessela, B.C.
    Engineering the L-arabinose isomerase from Enterococcus faecium for D-tagatose synthesis (2017), Molecules, 22, 2164 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.4 expression in Escherichia coli Enterococcus faecium

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.4 additional information expression of N- and C-terminal His-tagged protein in Escherichia coli. The C-His-tagged enzyme is preferentially hexameric in solution, whereas the N-His-tagged protein is mainly monomeric. The N-His-tagged variant shows a maximum bioconversion yield of 26% at 50°C for D-tagatose biosynthesis, the C-His-tagged variant is more active and stable at alkaline pH than the N-His-tagged variant Enterococcus faecium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.4 252
-
 D-galactose N-His-tagged recombinant protein, Michaelis-Menten kinetics, pH 5.6, 50°C Enterococcus faecium
5.3.1.4 426
-
 D-galactose C-His-tagged recombinant protein, K0.5 value, cooperative interaction model, pH 5.6, 50°C Enterococcus faecium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.4 28400
-
 plus 129000 and 53000, C-His-tagged recombinant protein, gel filtration Enterococcus faecium
5.3.1.4 52000
-
 plus 181000 and 311000, N-His-tagged recombinant protein, gel filtration Enterococcus faecium
5.3.1.4 53000
-
 plus 129000 and 284000, C-His-tagged recombinant protein, gel filtration Enterococcus faecium
5.3.1.4 129000
-
 plus 284000 and 53000, C-His-tagged recombinant protein, gel filtration Enterococcus faecium
5.3.1.4 181000
-
 plus 311000 and 52000, N-His-tagged recombinant protein, gel filtration Enterococcus faecium
5.3.1.4 311000
-
 plus 181000 and 52000, N-His-tagged recombinant protein, gel filtration Enterococcus faecium

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.4 Enterococcus faecium A0A1L2F0V9
-
-
5.3.1.4 Enterococcus faecium DBFIQ E36 A0A1L2F0V9
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.4 1.86
-
 substrate D-galactose, C-His-tagged recombinant protein, pH 5.6, 50°C Enterococcus faecium
5.3.1.4 2.77
-
 substrate D-galactose, N-His-tagged recombinant protein, pH 5.6, 50°C Enterococcus faecium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.4 D-galactose
-
 Enterococcus faecium D-tagatose
-
 ?
5.3.1.4 D-galactose
-
 Enterococcus faecium DBFIQ E36 D-tagatose
-
 ?
5.3.1.4 L-arabinose
-
 Enterococcus faecium L-ribulose
-
 ?
5.3.1.4 L-arabinose
-
 Enterococcus faecium DBFIQ E36 L-ribulose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.4 50
-
 wild-type, presence of Mn2+ Enterococcus faecium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.4 5.6
-
 wild-type, presence of Mn2+ Enterococcus faecium