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Literature summary extracted from

  • Palmer, D.J.; Schroeder, S.; Lawrence, A.D.; Deery, E.; Lobo, S.A.; Saraiva, L.M.; McLean, K.J.; Munro, A.W.; Ferguson, S.J.; Pickersgill, R.W.; Brown, D.G.; Warren, M.J.
    The structure, function and properties of sirohaem decarboxylase - an enzyme with structural homology to a transcription factor family that is part of the alternative haem biosynthesis pathway (2014), Mol. Microbiol., 93, 247-261 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.111 expressed in Escherichia coli BL21(DE3) cells Methanosarcina barkeri
4.1.1.111 expressed in Escherichia coli BL21(DE3) cells Desulfovibrio desulfuricans
4.1.1.111 expressed in Escherichia coli BL21(DE3) cells Desulfovibrio vulgaris

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.111 hanging drop vapor diffusion method, using 0.2 M ammonium acetate, 0.1 M sodium acetate pH 4.5, 30% (w/v) PEG 4000, 0.01 M BaCl2 Desulfovibrio desulfuricans

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.111 siroheme
-
 Desulfovibrio desulfuricans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.111 0.01174
-
 siroheme pH and temperature not specified in the publication Desulfovibrio desulfuricans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.111 39000
-
 calculated from amino acid sequence Desulfovibrio desulfuricans
4.1.1.111 40000
-
 gel filtration Methanosarcina barkeri
4.1.1.111 40000
-
 gel filtration Desulfovibrio desulfuricans
4.1.1.111 40000
-
 gel filtration Desulfovibrio vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.111 siroheme Methanosarcina barkeri
-
 12,18-didecarboxysiroheme + CO2
-
 ?
4.1.1.111 siroheme Desulfovibrio desulfuricans
-
 12,18-didecarboxysiroheme + CO2
-
 ?
4.1.1.111 siroheme Desulfovibrio vulgaris
-
 12,18-didecarboxysiroheme + CO2
-
 ?
4.1.1.111 siroheme Desulfovibrio desulfuricans ATCC27774
-
 12,18-didecarboxysiroheme + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.111 Desulfovibrio desulfuricans
-
-
-
4.1.1.111 Desulfovibrio desulfuricans ATCC27774
-
-
-
4.1.1.111 Desulfovibrio vulgaris
-
-
-
4.1.1.111 Methanosarcina barkeri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.111 Ni-Sepharose column chromatography and Superdex 200 gel filtration Methanosarcina barkeri
4.1.1.111 Ni-Sepharose column chromatography and Superdex 200 gel filtration Desulfovibrio desulfuricans
4.1.1.111 Ni-Sepharose column chromatography and Superdex 200 gel filtration Desulfovibrio vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.111 siroheme
-
 Methanosarcina barkeri 12,18-didecarboxysiroheme + CO2
-
 ?
4.1.1.111 siroheme
-
 Desulfovibrio desulfuricans 12,18-didecarboxysiroheme + CO2
-
 ?
4.1.1.111 siroheme
-
 Desulfovibrio vulgaris 12,18-didecarboxysiroheme + CO2
-
 ?
4.1.1.111 siroheme
-
 Desulfovibrio desulfuricans ATCC27774 12,18-didecarboxysiroheme + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.111 heterodimer
-
 Methanosarcina barkeri
4.1.1.111 heterodimer
-
 Desulfovibrio desulfuricans
4.1.1.111 heterodimer
-
 Desulfovibrio vulgaris

Synonyms

EC Number Synonyms Comment Organism
4.1.1.111 AhbA subunit Methanosarcina barkeri
4.1.1.111 AhbA subunit Desulfovibrio desulfuricans
4.1.1.111 AhbA subunit Desulfovibrio vulgaris
4.1.1.111 AhbB subunit Methanosarcina barkeri
4.1.1.111 AhbB subunit Desulfovibrio desulfuricans
4.1.1.111 AhbB subunit Desulfovibrio vulgaris
4.1.1.111 sirohaem decarboxylase
-
 Methanosarcina barkeri
4.1.1.111 sirohaem decarboxylase
-
 Desulfovibrio desulfuricans
4.1.1.111 sirohaem decarboxylase
-
 Desulfovibrio vulgaris

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.111 0.00177
-
 siroheme pH and temperature not specified in the publication Desulfovibrio desulfuricans