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Literature summary extracted from

  • Zhou, Y.; Memelink, J.; Linthorst, H.J.M.
    An E. coli biosensor for screening of cDNA libraries for isochorismate pyruvate lyase-encoding cDNAs (2018), Mol. Genet. Genomics, 293, 1181-1190 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.99.21 gene prxr1, Arabidopsis thaliana cDNA library screening using XL1-LUX, recombinant expression of His-tagged PRXR1 protein in Escherichia coli Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
4.2.99.21 additional information development of an Escherichia coli salicylate (SA) biosensor to screen for IPL activity based on the SalR regulator/psalA promoter combination from Acinetobacter sp. ADP1, to control the expression of the reporter luxCDABE. The sensing components, including the SA-inducible promoter PsalA, the reporter operon luxCDABE and the salR gene encoding the LysR-type regulator of salA, are subcloned from Acinetobacter ADPWH_lux into a plasmid vector. The biosensor is responsive to micromolar concentrations of exogenous SA, and to endogenous SA produced after transformation with a plasmid permitting IPTG-inducible expression of bacterial IPL in this biosensor strain. After screening a cDNA library constructed from turnip crinkle virus (TCV)-infected Arabidopsis thaliana ecotype Di-17, an enzyme, PRXR1, is identified as a putative IPL that converts isochorismate into SA Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.99.21 Mg2+ required Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.99.21 isochorismate Arabidopsis thaliana
-
 salicylate + pyruvate
-
 ?
4.2.99.21 isochorismate Arabidopsis thaliana ecotype Di-17
-
 salicylate + pyruvate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.99.21 Arabidopsis thaliana
-
-
-
4.2.99.21 Arabidopsis thaliana ecotype Di-17
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.99.21 recombinant His-tagged PRXR1 protein from Escherichia coli by nickel affinity chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.99.21 isochorismate
-
 Arabidopsis thaliana salicylate + pyruvate
-
 ?
4.2.99.21 isochorismate
-
 Arabidopsis thaliana ecotype Di-17 salicylate + pyruvate
-
 ?
4.2.99.21 additional information incubation of chorismate with the combination of the recombinant proteins EntC (isochorismate synthase, EC 5.4.4.2) and His-PRXR1 results in enhanced levels of salicylate, in a His-PRXR1-dependent manner Arabidopsis thaliana ?
-
 ?
4.2.99.21 additional information incubation of chorismate with the combination of the recombinant proteins EntC (isochorismate synthase, EC 5.4.4.2) and His-PRXR1 results in enhanced levels of salicylate, in a His-PRXR1-dependent manner Arabidopsis thaliana ecotype Di-17 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.99.21 ? x * 38000, recombinant His-tagged PRXR1, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
4.2.99.21 IPL
-
 Arabidopsis thaliana
4.2.99.21 isochorismate pyruvate lyase
-
 Arabidopsis thaliana
4.2.99.21 PRXR1
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.99.21 30
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.99.21 7
-
 assay at Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
4.2.99.21 physiological function salicylic acid (SA) is an essential hormone for development and induced defense against biotrophic pathogens in plants. The formation of SA mainly derives from chorismate via isochorismate synthase (ICS, EC 5.4.4.2) and isochorismate pyruvate lyase (IPL, EC 4.2.99.21)-mediated steps in Arabidopsis thaliana Arabidopsis thaliana