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Literature summary extracted from

  • Pantazaki, A.; Ioannou, A.; Kyriakidis, D.
    A thermostable beta-ketothiolase of polyhydroxyalkanoates (PHAs) in Thermus thermophilus Purification and biochemical properties (2005), Mol. Cell. Biochem., 269, 27-36 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.9 Mg2+ dose-dependent stimulation of the thiolytic reaction Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.9 acetoacetyl CoA thiolysis reaction shows a substrate inhibition at high concentrations; when one of the substrates (acetoacetyl CoA or CoA) is varied, while the concentrations of the second substrates (CoA or acetoacetyl CoA respectively) remain constant Thermus thermophilus
2.3.1.9 CoA thiolysis reaction shows a substrate inhibition at high concentrations; when one of the substrates (acetoacetyl CoA or CoA) is varied, while the concentrations of the second substrates (CoA or acetoacetyl CoA respectively) remain constant Thermus thermophilus
2.3.1.9 N-ethylmaleimide 1 mM Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.9 0.011
-
 CoA pH 8.0, 65°C Thermus thermophilus
2.3.1.9 0.027
-
 acetoacetyl-CoA pH 8.0, 65°C Thermus thermophilus
2.3.1.9 0.25
-
 acetyl-CoA pH 8.0, 65°C Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.9 45500
-
 SDS-PAGE Thermus thermophilus
2.3.1.9 182000
-
 gel filtration Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.9 CoA + acetoacetyl-CoA Thermus thermophilus the enzyme of the biosynthetic pathway for polyhydroxyalkanoates 2 acetyl-CoA
-
 r

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.3.1.9 guanidine-HCl denaturation of the enzyme with 6 M guanidine hydrochloride at pH 8.0 and renaturation at pH 7.0 Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.9 Thermus thermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.9
-
 Thermus thermophilus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.1.9 denaturation of the enzyme with 6 M guanidine hydrochloride at pH 8.0 and renaturation at pH 7.0 Thermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.9 1082
-
 pH 8.0, 65°C, cleavage of acetoacetyl CoA Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.9 2 acetyl-CoA
-
 Thermus thermophilus CoA + acetoacetyl-CoA
-
 r
2.3.1.9 CoA + acetoacetyl-CoA
-
 Thermus thermophilus 2 acetyl-CoA
-
 r
2.3.1.9 CoA + acetoacetyl-CoA the enzyme of the biosynthetic pathway for polyhydroxyalkanoates Thermus thermophilus 2 acetyl-CoA
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.9 tetramer 4 * 45500, SDS-PAGE Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.9 beta-ketoacyl-CoA thiolase
-
 Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.9 65
-
 thiolysis reaction and condensation reaction Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.9 8
-
-
 Thermus thermophilus

General Information

EC Number General Information Comment Organism
2.3.1.9 metabolism the enzyme of the biosynthetic pathway for polyhydroxyalkanoates Thermus thermophilus