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Literature summary extracted from

  • Kao, C.H.; Chen, Y.Y.; Wang, L.R.; Lee, Y.C.
    Production of N-acetyl-D-neuraminic acid by recombinant single whole cells co-expressing N-acetyl-D-glucosamine-2-epimerase and N-acetyl-D-neuraminic acid aldolase (2018), Mol. Biotechnol., 60, 427-434 .
    View publication on PubMed

Application

EC Number Application Comment Organism
5.1.3.8 synthesis recombinant Escherichia coli cells synchronously expressing N-acetyl-D-glucosamine-2-epimerase and N-acetyl-D-neuraminic acid aldolase as biocatalysts can potentially be used in the industrial mass production of Neu5Ac Anabaena sp. CH1

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.8 gene bage, recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3), coexpression with N-acetyl-D-neuraminic acid aldolase gene nanA from Escherichia coli Anabaena sp. CH1

Protein Variants

EC Number Protein Variants Comment Organism
5.1.3.8 additional information production of N-acetyl-D-neuraminic acid by recombinant single whole cells co-expressing N-acetyl-D-glucosamine-2-epimerase and N-acetyl-D-neuraminic acid aldolase in Escherichia coli. Method development and evaluation, overview Anabaena sp. CH1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.3.8 N-acetyl-D-glucosamine Anabaena sp. CH1
-
 N-acetyl-D-mannosamine
-
 r
5.1.3.8 N-Acyl-D-glucosamine Anabaena sp. CH1
-
 N-Acyl-D-mannosamine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.8 Anabaena sp. CH1 A4UA16
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.8 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, copurification with recombinant Escherichia coli N-acetyl-D-neuraminic acid aldolase Anabaena sp. CH1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.8 N-acetyl-D-glucosamine
-
 Anabaena sp. CH1 N-acetyl-D-mannosamine
-
 r
5.1.3.8 N-Acyl-D-glucosamine
-
 Anabaena sp. CH1 N-Acyl-D-mannosamine
-
 r

Subunits

EC Number Subunits Comment Organism
5.1.3.8 ? x * 42000, recombinant His-tagged enzyme, SDS-PAGE Anabaena sp. CH1

Synonyms

EC Number Synonyms Comment Organism
5.1.3.8 AGE
-
 Anabaena sp. CH1
5.1.3.8 bage
-
 Anabaena sp. CH1
5.1.3.8 GlcNAc-2-epimerase
-
 Anabaena sp. CH1
5.1.3.8 N-acetyl-D-glucosamine-2-epimerase
-
 Anabaena sp. CH1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.3.8 37
-
 assay at Anabaena sp. CH1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.8 8
-
 assay at Anabaena sp. CH1