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Literature summary extracted from

  • Xu, W.; Fan, C.; Zhang, T.; Jiang, B.; Mu, W.
    Cloning, expression, and characterization of a novel L-arabinose isomerase from the psychrotolerant bacterium Pseudoalteromonas haloplanktis (2016), Mol. Biotechnol., 58, 695-706 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.4 expression in Escherichia coli Pseudoalteromonas haloplanktis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.1.4 homology modeling of structure Pseudoalteromonas haloplanktis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.4 111.7
-
 L-arabinose pH 7.0, 40°C Pseudoalteromonas haloplanktis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.4 Co2+ 1 mM, 4.2fold activation Pseudoalteromonas haloplanktis
5.3.1.4 Mn2+ 1 mM, 3.4fold activation Pseudoalteromonas haloplanktis

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.4 Pseudoalteromonas haloplanktis Q3IG38
-
-
5.3.1.4 Pseudoalteromonas haloplanktis ATCC 14393 Q3IG38
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.4 5.2
-
 substrate L-arabinose, pH 7.0, 40°C Pseudoalteromonas haloplanktis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.4 L-arabinose
-
 Pseudoalteromonas haloplanktis L-ribulose
-
 ?
5.3.1.4 L-arabinose
-
 Pseudoalteromonas haloplanktis ATCC 14393 L-ribulose
-
 ?
5.3.1.4 additional information no substrate: D-galactose Pseudoalteromonas haloplanktis ?
-
 ?
5.3.1.4 additional information no substrate: D-galactose Pseudoalteromonas haloplanktis ATCC 14393 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.4 ? x * 55000, SDS-PAGE, x * 55014, calculated from sequence Pseudoalteromonas haloplanktis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.4 40
-
-
 Pseudoalteromonas haloplanktis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.3.1.4 45
-
 94% of maximum activity Pseudoalteromonas haloplanktis
5.3.1.4 60
-
 15% of maximum activity Pseudoalteromonas haloplanktis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.4 30
-
 2 h, 80% residual activity Pseudoalteromonas haloplanktis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.4 12.9
-
 L-arabinose pH 7.0, 40°C Pseudoalteromonas haloplanktis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.4 8
-
-
 Pseudoalteromonas haloplanktis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.4 7.5 9 more than 75% of maximum activity Pseudoalteromonas haloplanktis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.1.4 0.11
-
 L-arabinose pH 7.0, 40°C Pseudoalteromonas haloplanktis