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Literature summary extracted from

  • Dhillon, A.; Fernandes, V.O.; Dias, F.M.; Prates, J.A.; Ferreira, L.M.; Fontes, C.M.; Centeno, M.S.; Goyal, A.
    A New member of family 11 polysaccharide lyase, rhamnogalacturonan lyase (CtRGLf) from Clostridium thermocellum (2016), Mol. Biotechnol., 58, 232-240 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.23 expression in Escherichia coli Acetivibrio thermocellus

Protein Variants

EC Number Protein Variants Comment Organism
4.2.2.23 additional information expression of a protein derivative without the N-terminal Dockerin I. Truncated protein has a molecular mass of 63900 Da Acetivibrio thermocellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.23 additional information
-
 pectic galactan Km value of wild-type 10 mg/ml, of truncated mutant 11.2 mg/ml, respectively, pH 8.5, 70°C Acetivibrio thermocellus
4.2.2.23 additional information
-
 potato galactan Km value of wild-type 4.7 mg/ml, of truncated mutant 6.4 mg/ml, respectively, pH 8.5, 70°C Acetivibrio thermocellus
4.2.2.23 additional information
-
 soybean rhamnogalacturonan I Km value of wild-type 4.8 mg/ml, of truncated mutant 5.1 mg/ml, respectively, pH 8.5, 70°C Acetivibrio thermocellus
4.2.2.23 additional information
-
 potato rhamnogalacturonan I Km value of wild-type 4.9 mg/ml, of truncated mutant 6.0 mg/ml, respectively, pH 8.5, 70°C Acetivibrio thermocellus
4.2.2.23 additional information
-
 Pectin Km value of wild-type 8.2 mg/ml, of truncated mutant 7.6 mg/ml, respectively, pH 8.5, 70°C Acetivibrio thermocellus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.23 Ca2+ 3 mM, 1.5fold activation of wild-type, 1.3fold activation of truncated mutant protein of 63900 Da Acetivibrio thermocellus

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.23 Acetivibrio thermocellus A3DC06
-
-
4.2.2.23 Acetivibrio thermocellus DSM 1237 A3DC06
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.23 4.5
-
 substrate soybean rhamnogalacturonan I, pH 8.5, temperature not specified in the publication Acetivibrio thermocellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.23 pectic galactan
-
 Acetivibrio thermocellus ?
-
 ?
4.2.2.23 pectic galactan
-
 Acetivibrio thermocellus DSM 1237 ?
-
 ?
4.2.2.23 pectin 25% methyl-esterified Acetivibrio thermocellus ?
-
 ?
4.2.2.23 pectin 25% methyl-esterified Acetivibrio thermocellus DSM 1237 ?
-
 ?
4.2.2.23 potato galactan
-
 Acetivibrio thermocellus ?
-
 ?
4.2.2.23 potato galactan
-
 Acetivibrio thermocellus DSM 1237 ?
-
 ?
4.2.2.23 potato rhamnogalacturonan I
-
 Acetivibrio thermocellus rhamnogalacturonan I oligosaccharides with alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end
-
 ?
4.2.2.23 potato rhamnogalacturonan I
-
 Acetivibrio thermocellus DSM 1237 rhamnogalacturonan I oligosaccharides with alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end
-
 ?
4.2.2.23 soybean rhamnogalacturonan I
-
 Acetivibrio thermocellus rhamnogalacturonan I oligosaccharides with alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end
-
 ?
4.2.2.23 soybean rhamnogalacturonan I
-
 Acetivibrio thermocellus DSM 1237 rhamnogalacturonan I oligosaccharides with alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.2.23 RglF
-
 Acetivibrio thermocellus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.23 60
-
 truncated mutant protein of 63900 Da Acetivibrio thermocellus
4.2.2.23 70
-
 wild-type Acetivibrio thermocellus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.2.23 2.1
-
 potato galactan truncated mutant, pH 8.5, 70°C Acetivibrio thermocellus
4.2.2.23 2.3
-
 Pectin truncated mutant, pH 8.5, 70°C Acetivibrio thermocellus
4.2.2.23 4.8
-
 pectic galactan truncated mutant, pH 8.5, 70°C Acetivibrio thermocellus
4.2.2.23 6
-
 potato rhamnogalacturonan I truncated mutant, pH 8.5, 70°C Acetivibrio thermocellus
4.2.2.23 6.4
-
 potato galactan wild-type, pH 8.5, 70°C Acetivibrio thermocellus
4.2.2.23 6.9
-
 soybean rhamnogalacturonan I truncated mutant, pH 8.5, 70°C Acetivibrio thermocellus
4.2.2.23 7.7
-
 pectic galactan wild-type, pH 8.5, 70°C Acetivibrio thermocellus
4.2.2.23 9.8
-
 Pectin wild-type, pH 8.5, 70°C Acetivibrio thermocellus
4.2.2.23 21
-
 potato rhamnogalacturonan I wild-type, pH 8.5, 70°C Acetivibrio thermocellus
4.2.2.23 21.2
-
 soybean rhamnogalacturonan I wild-type, pH 8.5, 70°C Acetivibrio thermocellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.23 8.5
-
-
 Acetivibrio thermocellus

General Information

EC Number General Information Comment Organism
4.2.2.23 physiological function both the full-length Rgl protein (80000 Da) and its truncated derivative (63900 Da) display maximum activity against rhamnogalacturonan I Acetivibrio thermocellus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.2.23 additional information
-
 pectic galactan kcat/Km value of wild-type 0.77 ml/mg/s, of truncated mutant 0.43 ml/mg/s, respectively, pH 8.5, 70°C Acetivibrio thermocellus
4.2.2.23 additional information
-
 Pectin kcat/Km value of wild-type 1.19 ml/mg/s, of truncated mutant 0.3 ml/mg/s, respectively, pH 8.5, 70°C Acetivibrio thermocellus
4.2.2.23 additional information
-
 potato galactan kcat/Km value of wild-type 1.36 ml/mg/s, of truncated mutant 0.32 ml/mg/s, respectively, pH 8.5, 70°C Acetivibrio thermocellus
4.2.2.23 additional information
-
 soybean rhamnogalacturonan I kcat/Km value of wild-type 4.1 ml/mg/s, of truncated mutant 1.35 ml/mg/s, respectively, pH 8.5, 70°C Acetivibrio thermocellus
4.2.2.23 additional information
-
 potato rhamnogalacturonan I kcat/Km value of wild-type 4.28 ml/mg/s, of truncated mutant 1.13 ml/mg/s, respectively, pH 8.5, 70°C Acetivibrio thermocellus