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Literature summary extracted from

  • Gruendel, M.; Knoop, H.; Steuer, R.
    Activity and functional properties of the isocitrate lyase in the cyanobacterium Cyanothece sp. PCC 7424 (2017), Microbiology, 163, 731-744 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.1 expressed in Escherichia coli BL21(DE3) cells Gloeothece citriformis PCC 7424

General Stability

EC Number General Stability Organism
4.1.3.1 freezing and thawing reduce the enzyme activity significantly Gloeothece citriformis PCC 7424

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.1 phosphoenolpyruvate 90% inhibition at 2 mM Gloeothece citriformis PCC 7424
4.1.3.1 succinate 90% inhibition at 5 mM Gloeothece citriformis PCC 7424

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.1 0.06
-
 DL-isocitrate at pH 7.4 and 25°C Gloeothece citriformis PCC 7424

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.1 Mg2+ required, in the absence of Mg2+, no enzymatic activity is measured Gloeothece citriformis PCC 7424

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.1 isocitrate Gloeothece citriformis PCC 7424
-
 succinate + glyoxylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.1 Gloeothece citriformis PCC 7424
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.1 glutathione agarose column chromatography Gloeothece citriformis PCC 7424

Storage Stability

EC Number Storage Stability Organism
4.1.3.1 4°C, 50 mM Tris/HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol, few days, the enzyme remains stable Gloeothece citriformis PCC 7424

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.1 DL-isocitrate
-
 Gloeothece citriformis PCC 7424 succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate
-
 Gloeothece citriformis PCC 7424 succinate + glyoxylate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.1 ? x * 54000, SDS-PAGE Gloeothece citriformis PCC 7424
4.1.3.1 ? x * 54500, calculated from amino acid sequence Gloeothece citriformis PCC 7424

Synonyms

EC Number Synonyms Comment Organism
4.1.3.1 ICL
-
 Gloeothece citriformis PCC 7424

Expression

EC Number Organism Comment Expression
4.1.3.1 Gloeothece citriformis PCC 7424 enzyme activity is induced under alternating day/night cycles up