BRENDA - Enzyme Database

Activity and functional properties of the isocitrate lyase in the cyanobacterium Cyanothece sp. PCC 7424

Gruendel, M.; Knoop, H.; Steuer, R.; Microbiology 163, 731-744 (2017)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.1.3.1
expressed in Escherichia coli BL21(DE3) cells
Cyanothece sp. PCC 7424
General Stability
EC Number
General Stability
Organism
4.1.3.1
freezing and thawing reduce the enzyme activity significantly
Cyanothece sp. PCC 7424
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
phosphoenolpyruvate
90% inhibition at 2 mM
Cyanothece sp. PCC 7424
4.1.3.1
succinate
90% inhibition at 5 mM
Cyanothece sp. PCC 7424
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.06
-
DL-isocitrate
at pH 7.4 and 25C
Cyanothece sp. PCC 7424
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Mg2+
required, in the absence of Mg2+, no enzymatic activity is measured
Cyanothece sp. PCC 7424
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Cyanothece sp. PCC 7424
-
succinate + glyoxylate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Cyanothece sp. PCC 7424
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
glutathione agarose column chromatography
Cyanothece sp. PCC 7424
Storage Stability
EC Number
Storage Stability
Organism
4.1.3.1
4C, 50 mM Tris/HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol, few days, the enzyme remains stable
Cyanothece sp. PCC 7424
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
DL-isocitrate
-
748615
Cyanothece sp. PCC 7424
succinate + glyoxylate
-
-
-
?
4.1.3.1
isocitrate
-
748615
Cyanothece sp. PCC 7424
succinate + glyoxylate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.1.3.1
?
x * 54000, SDS-PAGE; x * 54500, calculated from amino acid sequence
Cyanothece sp. PCC 7424
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
expressed in Escherichia coli BL21(DE3) cells
Cyanothece sp. PCC 7424
General Stability (protein specific)
EC Number
General Stability
Organism
4.1.3.1
freezing and thawing reduce the enzyme activity significantly
Cyanothece sp. PCC 7424
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
phosphoenolpyruvate
90% inhibition at 2 mM
Cyanothece sp. PCC 7424
4.1.3.1
succinate
90% inhibition at 5 mM
Cyanothece sp. PCC 7424
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.06
-
DL-isocitrate
at pH 7.4 and 25C
Cyanothece sp. PCC 7424
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Mg2+
required, in the absence of Mg2+, no enzymatic activity is measured
Cyanothece sp. PCC 7424
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Cyanothece sp. PCC 7424
-
succinate + glyoxylate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
glutathione agarose column chromatography
Cyanothece sp. PCC 7424
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.1.3.1
4C, 50 mM Tris/HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol, few days, the enzyme remains stable
Cyanothece sp. PCC 7424
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
DL-isocitrate
-
748615
Cyanothece sp. PCC 7424
succinate + glyoxylate
-
-
-
?
4.1.3.1
isocitrate
-
748615
Cyanothece sp. PCC 7424
succinate + glyoxylate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.3.1
?
x * 54000, SDS-PAGE; x * 54500, calculated from amino acid sequence
Cyanothece sp. PCC 7424
Expression
EC Number
Organism
Commentary
Expression
4.1.3.1
Cyanothece sp. PCC 7424
enzyme activity is induced under alternating day/night cycles
up
Expression (protein specific)
EC Number
Organism
Commentary
Expression
4.1.3.1
Cyanothece sp. PCC 7424
enzyme activity is induced under alternating day/night cycles
up