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Literature summary extracted from

  • Wang, Y.; Percival, Y.
    Overexpression and simple purification of the Thermotoga maritima 6-phosphogluconate dehydrogenase in Escherichia coli and its application for NADPH regeneration (2009), Microb. Cell Fact., 8, 30 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.1.1.44 synthesis NADPH regeneration. When coupled with glucose-6-phosphate dehydrogenase the enzyme generates two moles of NADPH per mole of glucose-6-phosphate Thermotoga maritima

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.44 overexpression in Escherichia coli Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.44 0.01
-
 NADP+ 80°C, pH 7.5 Thermotoga maritima
1.1.1.44 0.011
-
 6-phospho-D-gluconate 80°C, pH 7.5 Thermotoga maritima
1.1.1.44 0.38
-
 NAD+ 80°C, pH 7.5 Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.44 Thermotoga maritima Q9WYR9
-
-
1.1.1.44 Thermotoga maritima DSM 3109 Q9WYR9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.44
-
 Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NAD+ the enzyme prefers NADP+ to NAD+ as an electron acceptor. Km-value for NAD+ is 38fold higher than the Km-value for NADP+ Thermotoga maritima D-ribulose 5-phosphate + CO2 + NADH + H+
-
 ?
1.1.1.44 6-phospho-D-gluconate + NAD+ the enzyme prefers NADP+ to NAD+ as an electron acceptor. Km-value for NAD+ is 38fold higher than the Km-value for NADP+ Thermotoga maritima DSM 3109 D-ribulose 5-phosphate + CO2 + NADH + H+
-
 ?
1.1.1.44 6-phospho-D-gluconate + NADP+ the enzyme preferrs NADP+ to NAD+ as an electron acceptor Thermotoga maritima D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?
1.1.1.44 6-phospho-D-gluconate + NADP+ the enzyme preferrs NADP+ to NAD+ as an electron acceptor Thermotoga maritima DSM 3109 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.44 6PGDH
-
 Thermotoga maritima
1.1.1.44 TM0438
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.44 90
-
-
 Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.44 70 95 70°C: about 55% of maximal activity, 95°C: about 65% of maximal activity Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.44 80
-
 half-life: 140 h Thermotoga maritima
1.1.1.44 90
-
 half-life: 48 h Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.44 325
-
 NADP+ 80°C, pH 7.5 Thermotoga maritima
1.1.1.44 325
-
 6-phospho-D-gluconate 80°C, pH 7.5 Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.44 7
-
-
 Thermotoga maritima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.44 6 9 about 70% of the activity remains at pH 6.0 and 9.0 Thermotoga maritima

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.44 NADP+ the enzyme preferrs NADP+ to NAD+ as an electron acceptor Thermotoga maritima

Expression

EC Number Organism Comment Expression
1.1.1.44 Thermotoga maritima through numerous approaches including expression vectors, hosts, cultivation conditions, inducers, and codon-optimization of the 6pgdh gene, the soluble 6PGDH expression levels are enhanced to about 250 mg per liter of culture by more than 500fold up